LOCUS       sequence-325402-        9766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Smo-FLAG K434R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: GP821
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(47..838,839..907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     670..689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(908..1012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1080..1098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             1145..1325
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1473..2061
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1579..1590
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1988..2008
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2102..2167)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      2167..2176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(4563..4571,4572..4574,4575..5148)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4736..4755)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4965..4982
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5149..5544)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5740..5874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5818..5837
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    5889..5980
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(5921..5940)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    5994..6042
                     /note="synthetic polyadenylation signal"
     misc_feature    6091..6208
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6504..6548)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6733..6966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7463..7588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7635..8268
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8529..8545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8529..8545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8542..8564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8553..8569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8577..8583,8584..8601,8602..8607))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8622..8643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8883..8900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9054..9642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
       61 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      661 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1021 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     1081 ataggcgtat cacgaggccc tttcgtcttc aagaatgatc tagccctttc cttaatcgcc
     1141 cgggtgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     1201 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     1261 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     1321 gacccagtac aagcaaaaag cagggcgcgc ctgtcctgca ggtgatcttg tcttcgttgg
     1381 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
     1441 gccttgtaag tcattggtct taaaggtacc aggcggggag gcggcccaaa gggagatccg
     1501 actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg
     1561 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag
     1621 aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac
     1681 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc
     1741 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca
     1801 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc
     1861 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa
     1921 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc
     1981 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat
     2041 tttgtaatcc agaggttgat taggatctct cgagttcgga tcttacttac taagcggccg
     2101 ccttgtcatc gtcatccttg taatcgatgt catgatcttt ataatcaccg tcatggtctt
     2161 tgtagtccat ggtggcgaag tccgagtctg catccaagat ctcagcctcc attaggttag
     2221 tgcgggaatg aatggatccc agcccctgga ggcggccctg agcccagacc cggggggctg
     2281 aggcaccagg gagaaatgga tcttgatggg gactaggctc tgggcagaag gggttggaga
     2341 ctagagtcca ggctccctgt cggcaggact cccctgttcc ccaggcgttt gcagctacca
     2401 ggcacttctg ccgaggcagc tgaggcagcc gaggaactgc actggtggca ggaacagggg
     2461 cagagtggtg aagctcaggg gctggcctca aggggcacac ctccttcttc ctcttcctcc
     2521 gctttttctt ccggcccaaa cgcttctcta actctgggga gatctctgcc tcaaccagcc
     2581 acatgttggc ttgttcttct ggtggcactg gagttgccac aggggtaacg gacacatcct
     2641 ggggcaatat ggctcctctc cgagccacca tcttggtgac atgctgagcc caggcagagg
     2701 agacgtcagc tgagggttca ttgaggtcaa aagccaaacc cgcaacaggt ccatcatggg
     2761 agacagtgtg catgctgaag gagagctcct ggcccgggtt ctgcagcagc tcacgccgct
     2821 tagagaaggc cttggcgatc atcttgctct tcttgattct cttgggctca tcatcactgt
     2881 gcccagtcaa cctgcaccag gtgcgcctcc agatgagcag ggtggccttg gtccagaccc
     2941 aggtgctcat ggcaatgcca gtgccaaaca tggcaaatag attgatcttc tccaccagga
     3001 ggctgggccg attcttgatc tcacagtcag gaatgggctt cttggtaggc agcccgatgg
     3061 tcacgttggc ttggcatagc acatagtccc ggaagctacg ctcccactca gcctggttga
     3121 agaagtcata gaagtggcag ctgaaggtga tgagcacaaa gccaaaggcc aggaagccaa
     3181 aaatgcccag gcgcagcatg gtctcgttga tcttgctggc tgccttctca ctcagaagcc
     3241 cagggtggtt gctcctgatg gagaacagag tcatgacccc tctgatgagg aagtagcctc
     3301 ccacaataag caccaggcca attggggcca ggacaaagcc agcacggtac cgatagttct
     3361 tgtagcctac aaaacagatg ccactcacgg agtctccatc tacctgagcc acagccagga
     3421 ttgccaccgt gaggacaaag gggagtgacc acgtgagcag gtggaaatag gatgtcttgc
     3481 ccgagagagg ctggtaggtg gtgcccaggg ctttgaagga ggtgtgccag gcataggtga
     3541 ggaccacgaa ccagactact ccagccatca aggcatagta cacaatgaca aagatgatga
     3601 cacaggacag ggtctcactg gaggtgggct ccccaaatct catggtgcca tctgctcggc
     3661 aaacaatctc tcggcgggca ccatccatga actgggccag ccagccaatg ctgcccacga
     3721 agaaacacgc attgacatag aagagaataa ccgcagggta gcgattggag ttccgccagt
     3781 cagccacaaa ggtggccagt gtgaagagcg tgcagagacc agtgaccgcc ccgaaggctg
     3841 cgatgtagct gtgcatgtcc tggtgctcgg cctcggtgaa cagcgggttc tgacactgaa
     3901 tcccacagcc ctccacgtcc tcataccagc tcttggggtt gtctgttcgc accaagggtg
     3961 cttcacattg gcctgagctg ttgaacttga tgttttgtac ctcgtttggg cagccttcag
     4021 ggaagtggtc cggtgtgcaa cgcagaaagt caggccaccc tcgctcccgc tccacaatgg
     4081 cacaggggcc tcgggtggcc tggcagaggg tacggctggg caactccact cggtcattct
     4141 cacacttggg catgtagaca gcacacagca ggggctggat cactgcccag cagcgggggg
     4201 cattccggag gccggaccag agcacgagct tgccgtgcgc ttcctcctgc gagtccgagt
     4261 ccccagccag cagcgtggtg gtggctccgt agggcagcgc cgagcccagg cacacgttgt
     4321 agcgcaaagg ctcgcagtgg gcggcccggc cgcagtggct cagcagcggc ggcggagggc
     4381 tggtcaccgg cacgtccctc ctcgagctcc cgctggcgct gtgaggccca ggcccggtca
     4441 cgttcccgct caaggccgcc ccccggcccg ggccccccag cagtaccagc agcagcaact
     4501 gcagcagcct ccggggcgcc agctcgggcc cacgcacggg gcggccagcg gccatggtct
     4561 atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg
     4621 ggggcctaga cgttttttta acctcgacta aacacatgta aagcatgtgt accgaggccc
     4681 cagatcagat cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac
     4741 gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg
     4801 ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc
     4861 gccaggtggg gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga
     4921 agcttccaga ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag
     4981 aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac
     5041 attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta
     5101 ttccaagcgg cttcggccag taacgttagg gggggggagg gagagggggc cctcccacac
     5161 ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt
     5221 cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg
     5281 gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc
     5341 cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata
     5401 tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta
     5461 gtcttcagag atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc
     5521 ttcttgagac aaaggcttgg ccatattaat gtcgctccgt gctctccggt ctcacagccc
     5581 cgcttttacg tgccccggag cctgggctgg cagctcgtca cgccccattg gggcggggtg
     5641 tcccctcgtc cagcccagct ctgccccctc tccctctggc ccccctcatg ctgcccaccc
     5701 ctatcctgtg tgtccactag tcgcgaatct gttcccgggg atccagacat gataagatac
     5761 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5821 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttcggtac
     5881 ctatcgatag agaaatgttc tggcacctgc acttgcactg gggacagcct attttgctag
     5941 tttgttttgt ttcgttttgt tttgatggag agcgtatgtt agtactatcg attcacacaa
     6001 aaaaccaaca cacagatgta atgaaaataa agatatttta ttgcggccac tagtccaaac
     6061 tggatctctg ctgtccgagt aataaacccg aaaattttga atttttgtaa tttgtttttg
     6121 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     6181 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgcctatt gttgagaatt
     6241 cggcgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
     6301 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
     6361 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     6421 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     6481 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     6541 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     6601 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     6661 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     6721 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     6781 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     6841 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     6901 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     6961 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     7021 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     7081 tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg
     7141 tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct
     7201 ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt
     7261 ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg
     7321 tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat
     7381 gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc
     7441 taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc
     7501 tagtcaaaat ttttggcgta ctctgcagtc gccgcccctc gcctcttgcc gtgcgcgctt
     7561 cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc
     7621 tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga gggatctcta
     7681 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     7741 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     7801 taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc
     7861 gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct
     7921 tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt
     7981 gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca
     8041 tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta
     8101 ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga
     8161 cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc
     8221 acagatcaag gatatcttgt cttctttggg agtgaattag cccttccaac tactaagttt
     8281 gtagtacata tttaacaaat acaatttctt taaaatgaaa ataattcaga ggaatcacag
     8341 gtttagagta aatgaaacca caggtaattg gcagtggtaa tagggtatgg ggtgggaagt
     8401 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
     8461 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattc
     8521 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8581 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccacggggag
     8701 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     8761 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttgctg
     8821 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9481 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9601 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9661 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9721 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaa
//
