LOCUS       sequence-32542-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_TO_FUS
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             184..207
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             208..234
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    250..274
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             681..692
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tagcagagct ctcctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
      181 atggactaca aggacgacga tgacaagtac ccttatgacg tgcccgatta cgctttgaac
      241 cagatatcaa caagtttgta caaaaaagca ggctccacca tgggaaccaa ttcagtcgac
      301 atggcctcaa acgattatac ccaacaagca acccaaagct atggggccta ccccacccag
      361 cccgggcagg gctattccca gcagagcagt cagccctacg gacagcagag ttacagtggt
      421 tatagccagt ccacggacac ttcaggatat ggccagagca gctattcttc ttatggccag
      481 agccagaaca caggctatgg aactcagtca actccccagg gatatggctc gactggcggc
      541 tatggcagta gccagagctc ccaatcgtct tacgggcagc agtcctccta ccctggctat
      601 ggccagcagc cagctcccag cagcacctcg ggaagttacg gtagcagttc tcagagcagc
      661 agctatgggc agccccagag tgggagctac agccagcagc ctagctatgg tggacagcag
      721 caaagctatg gacagcagca aagctataat ccccctcagg gctatggaca gcagaaccag
      781 tacaacagca gcagtggtgg tggaggtgga ggtggaggtg gaggtaacta tggccaagat
      841 caatcctcca tgagtagtgg tggtggcagt ggtggcggtt atggcaatca agaccagagt
      901 ggtggaggtg gcagcggtgg ctatggacag caggaccgt
//
