LOCUS       sequence-325424-        9622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration of 2 LoxN sites in the genome of P. berghei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9622)
  TITLE     Silvie lab unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUpstream2Lox
FEATURES             Location/Qualifiers
     source          1..9622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(22..55)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             2086..2796
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2086..2796
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2113..2141)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2736..2756
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2806..2859
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(4087..4120)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     complement(6782..6802)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6782..6800)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6821..6837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6821..6837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6834..6856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6845..6861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6869..6875,6876..6893,6894..6899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6914..6935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7052..7069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7223..7811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7303..7322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7982..8773,8774..8842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8605..8624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8843..8947)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8973..9428)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9110..9131)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9322..9341
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9555..9577
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9569..9586
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9570..9586
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9596..9615
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9596..9614
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtaccactc tcgaggaatt cataacttcg tatagtatac cttatacgaa gttatgatat
       61 caattctata aaaatagcat aatataaaat atattattta aattgctata cccaataata
      121 tacttattct ataatagcta tattattaat tttttttttt ttttttttca tcctctttat
      181 atatacatgt gtatattttc ctcagctttc aaataacaat tcgttattaa agagtcaata
      241 ttattttaaa aaaccaaaaa aaagagtgat gaaatataat aataaacaaa ttaactaact
      301 gagcttaaat tgtaaggaag atgatgtatg tgtccgtctt attacatttt agtccttttt
      361 taatttaatt taatgtcctt taaattttca aaacatattt taatataatt atcatattca
      421 aagaattgcc ataatattcg ataaaatgac attattgttg ttttcayttt tttttttttt
      481 tttttttttt tttttcattt ataattaaaa aggtgtattt taatgtatat aaaaaaggaa
      541 gtatattata tatataagaa taagatagga ataatacatg agatggttta tttatttgtt
      601 tgtttaacca atttgaggga aaatatacgt atgtacatac tttgctagtg agcttaaaaa
      661 aaacttttaa tattgtttgt gaggttaaag aaagcattta atattgtttg tgaggttaaa
      721 gaaaactttt aatattgttt gtgaaaattt tttactaaga acataatttt taagggatat
      781 tataaattta ttgaatttgt tattataaag taaattttat tttctgccta ttttatttat
      841 gtacaaaaaa ttgcggaatt gtacataata taatatgaaa aatatatgac tttaaattgc
      901 aattggcaaa taaggagggg gccccacgcc ctcaaagggt gccatacact gttgggtaat
      961 atggcagcaa tccatatcat taacactatc attatcttaa cattatcatt aacattatct
     1021 taacattatc attaacatat tgctttattc gcattaacgt taacatattg cgctctttca
     1081 gcccatgcct tgcaaataat tgcactttcg gaaatttgaa aaaatataaa gcccataaat
     1141 aaaggtggaa tataaaacat ttattgcatt ttcgaagcat ataaataaat atgtatataa
     1201 ttatgcatac tatatatatg tatatatttg actatctttt ccaataaaaa aaaaaaaaaa
     1261 aaaattatat aagggggccc atatatatag gcatattaat agctgcttta actaaagggg
     1321 ctaataaatg gccccgtatt ttgttttttt ttatagctga caataaaaat aaaaagtacg
     1381 aactttagtc ccataaatta agaactatat atgcttataa aaagcatata aaataaatta
     1441 atattaattt aaataaatta attatatgca aaaaaatatt taaaaaagaa tatatataaa
     1501 gaaaaataat tataaatatt atcattatta ttatacatat atatatacac cccttttgat
     1561 tatatatttt ttagagaaaa aaataaatag tgtattattt cctataatcg tagttaatta
     1621 attttaataa tttacaatac actatatatt atttttatta tatataaaat attatttata
     1681 attaattttt tatatttata tgtacatatt ttaattgcat cctttttaat aaaaatttat
     1741 ttacccaatt gttatagagc ttatttatta tataacttaa aaaaaattaa gatatttttt
     1801 attttttttt aaacatttat ttccaattta tttttaacgg tcaaattaaa gaaatatata
     1861 tatttattgc ttaaatttat aagtatatat ttaaaaaaaa aaattaaagc taaaaatata
     1921 tttcattgta ttttgcattt acgcaattcg ctactatttt gccataagta cattataata
     1981 tatgcttgta gcaaattaat taaataaaaa ataattacaa aaatttatta tcccaataaa
     2041 ttataattgc aattaaaaaa aaaacctagg aaaatgctta aggctagtaa aggagaagaa
     2101 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     2161 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     2221 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcgcgtat
     2281 ggtcttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     2341 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     2401 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     2461 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     2521 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     2581 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     2641 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     2701 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2761 gctgggatta cacatggcat ggatgaacta tacaaaggtt cgggagaggg cagaggatcc
     2821 ctgctaacat gcggtgatgt cgaggagaat cctggcccaa tggttggttc gctaaactgc
     2881 atcgtcgctg tgtcccagaa catgggcatc ggcaagaacg gggacctgcc ctggccaccg
     2941 ctcaggaacg aatttagata tttccagaga atgaccacaa cctcttcagt agaaggtaaa
     3001 cagaatctgg tgattatggg taagaagacc tggttctcca ttcctgagaa gaatcgacct
     3061 ttaaagggta gaattaattt agttctcagc agagaactca aggaacctcc acaaggagct
     3121 cattttcttt ccagaagtct agatgatgcc ttaaaactta ctgaacaacc agaattagca
     3181 aataaagtag acatggtctg gatagttggt ggcagttctg tttataagga agccatgaat
     3241 cacccaggcc atcttaaact atttgtgaca aggatcatgc aagactttga aagtgacacg
     3301 ttttttccag aaattgattt ggagaaatat aaacttctgc cagaataccc aggtgttctc
     3361 tctgatgtcc aggaggagaa aggcattaag tacaaatttg aagtatatga gaagaatgat
     3421 taaactagta ttattaatat atatgaatat atatacatcg ttgtatgcgt aatatacgtg
     3481 taactgtttt ttttaatttt tatattaact tttccttata cattttaatg ccattaacta
     3541 ataatatttt acttgaaaaa attctaaatt tttataactc taatataaaa tctgtgaata
     3601 ataaaaaatt agcataaagt acgaattaaa catcgaatat ttgttttaaa ggggcacaca
     3661 ttatgtgcat ataattttat tctttgaatt taattttttg cttataatta gattaaatta
     3721 atataatttt tttattgaaa ataaaaactt attttaaaaa attaacaaat ggaagttaat
     3781 gaaaaatacg aaaaaagtaa agggttaatt cttatatggt catataaaaa aacaaatgaa
     3841 ataatgtaaa cagttattca ttttttaagg gtataaaata agcttataaa taatgttagc
     3901 atattaaaaa cagtgatata agtatgtgta aatatgaaag aatataataa tggatagtta
     3961 aaaaaatata ttttaaaggg aatattgaca aattatatac tgaagcacta aagaaaatat
     4021 catctttgta tattatagct tcacatttcc aagcaaatgg tgccacaaat acattcttct
     4081 ctctccataa cttcgtatag tataccttat acgaagttat gctagcacta gttaataaag
     4141 ggcactttaa tttttatgca taattagctt atatttagtg catatttatt tttatacata
     4201 tttttatata ttttttgatt tacatggcgt tttatgttta tatgtataac attatatgca
     4261 tagtattttt ttgaaaattt aatggtattt cagtggatga aaatattact ggtgctttga
     4321 ggggtgagca tttaaagcac aatatctagg atactacaaa aaatacttaa agtatgaata
     4381 ttaaattgta aacttaagca taaagagctc gaaaagaatt aagcttaact catctacaag
     4441 catcaaatta aatgttttat gcagtacctt tttttaaata atccatttta tttttttata
     4501 ctccgaggat tttacacctg cactgacact acaaatatag taatatttta tccctacatt
     4561 atatgcattt tataatattt ttatttattt taataattta tacacataag tacatagtat
     4621 agagttgaaa aaaaaatacg tgtatttcta aaataaaatg aaataaatat tatgttatta
     4681 tattgaatac aataaacatt ttctccaaat taaaatttaa tattatataa caaaaaaaaa
     4741 tacaaaacac caattaaaaa taataataat tttatcctta ttttttaata actgccattt
     4801 ttaaagtata taataaaaaa taaattaaat tttaaaaata tttcaaaata taaaataaat
     4861 atataagaat atatatttgc ttataaataa ataaaaattt tatagtcgac aaaatggcaa
     4921 gcaagtggga tcagaagggt atggacattg cctatgagga ggcggcctta ggttacaagg
     4981 agggtggtgt tcctattggc ggatgtctta tcaataacaa agacggaagt gttctcggtc
     5041 gtggtcacaa catgagattt caaaagggat ccgccacact acatggtgag atctccactt
     5101 tggaaaactg tgggagatta gagggcaaag tgtacaaaga taccactttg tatacgacgc
     5161 tgtctccatg cgacatgtgt acaggtgcca tcatcatgta tggtattcca cgctgtgttg
     5221 tcggtgagaa cgttaatttc aaaagtaagg gcgagaaata tttacaaact agaggtcacg
     5281 aggttgttgt tgttgacgat gagaggtgta aaaagatcat gaaacaattt atcgatgaaa
     5341 gacctcagga ttggtttgaa gatattggtg aggcttcgga accatttaag aacgtctact
     5401 tgctacctca aacaaaccaa ttgctgggtt tgtacaccat catcagaaat aagaatacaa
     5461 ctagacctga tttcattttc tactccgata gaatcatcag attgttggtt gaagaaggtt
     5521 tgaaccatct acctgtgcaa aagcaaattg tggaaactga caccaacgaa aacttcgaag
     5581 gtgtctcatt catgggtaaa atctgtggtg tttccattgt cagagctggt gaatcgatgg
     5641 agcaaggatt aagagactgt tgtaggtctg tgcgtatcgg taaaatttta attcaaaggg
     5701 acgaggagac tgctttacca aagttattct acgaaaaatt accagaggat atatctgaaa
     5761 ggtatgtctt cctattagac ccaatgctgg ccaccggtgg tagtgctatc atggctacag
     5821 aagtcttgat taagagaggt gttaagccag agagaattta cttcttaaac ctaatctgta
     5881 gtaaggaagg gattgaaaaa taccatgccg ccttcccaga ggtcagaatt gttactggtg
     5941 ccctcgacag aggtctagat gaaaacaagt atctagttcc agggttgggt gactttggtg
     6001 acagatacta ctgtgtttaa gaattgatta ttgttctgta cttcttttgt gaatagtatt
     6061 tttacatatg tactgttaat tttaaaatgt ggaatgccac gaacatattt tttcgaacaa
     6121 aatatatatt ttttgtgtcg aaatacataa atttgtgtac ctcctttttg ttgtatatat
     6181 gaaaaaggaa taaagtgaat ggagacgaat tactctagac cctacaactt gtatatcact
     6241 actttataat actataatat tattattaac attaaactat agaagaaata tcaacaatac
     6301 ttatgtattt gttcaaactt tatcagtata gtttggtaat cgcatatcat atatttgtta
     6361 tgtgtaatat tttatgaata tgcgtctttt tttttaacta taaataattc aaaataaaat
     6421 aacataatta acatttgcaa gcataattaa gatgattact ttcacatttc atccaaagtg
     6481 gaaaatgacc accacatttg atcgaagctt ttaccacttt tgatgcatta gctctgaaat
     6541 gtatacaagc tttcatttgt taatgttaca aagggaagtt tttatttggg ggcacattaa
     6601 aagtgaaaaa attaaaagtt aaaaaagtta aaaaatgatc ggtgtagttg gtcatataga
     6661 catgttgtta tgcaagtctt tcaaaacgtt cctcataaaa taaaaggagg gatctagaga
     6721 gctttcaatt ctccaaacgg tattattggt atttgccgcg gtggagctcc agcttttgtt
     6781 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7621 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8941 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     9001 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     9061 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     9121 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     9181 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     9241 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     9301 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     9361 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     9421 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     9481 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     9541 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
     9601 cgactcacta tagggcgaat tg
//
