LOCUS       sequence-325440-       10984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Roy S, Torres-Jerez I, Zhang S, Liu W, Schiessl K, Jain D, Boschiero
            C, Lee HK, Krom N, Zhao PX, Murray JD, Oldroyd GED, Scheible WR, 
            Udvardi M
  TITLE     The peptide GOLVEN10 alters root development and noduletaxis in 
            Medicago truncatula.
  JOURNAL   Plant J. 2024 May;118(3):607-625. doi: 10.1111/tpj.16626. Epub 2024 
            Feb 15.
  PUBMED    38361340
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16626";
            journalName: "Plant J"; date: "2024-05"; volume: "118"; issue: "3"; 
            pages: "607-625"
COMMENT     Sequence Label: LjUBQ:MtGLV1:T35s
FEATURES             Location/Qualifiers
     source          1..10984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      216..468
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    2551..2725
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2825..3004
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3026..3820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3080..3099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3690..3709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      3833..4085
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4180..4204
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4468..5097
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5526..6599
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6665..6859
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        7142..7246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7247..8041
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8212..8800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8701..8720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8923..8947
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagagattca taaggctctg aagctgaaag acggtggtca ttacctagtt gaattcaaaa
       61 gtatttacat ggcaaagaag cctgtgcagc taccagggta ctactatgtt gactccaaac
      121 tggatataac aagccacaac gaagattata caatcgttga gcagtatgaa agaaccgagg
      181 gacgccacca tctgttcctt taagcttgtc aagcagatcg ttcaaacatt tggcaataaa
      241 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
      301 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
      361 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
      421 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga cgctgagctc
      481 gaattctagt ggagggagag aggattttga ggaaataatt aattgaatta cttgtattat
      541 tgataaagta atttagataa gttgttagta caacttattg caactatgga tagacaaaaa
      601 tcacttattt taaggggaga agtatataac aacttattat aaaatttctg agcagccggc
      661 ctcctccaat caatcataga gagtggagcc cattctggaa aaccaaggaa cccccaactt
      721 gcactggtgc ggtggcccaa ttcaaaaaaa acggggccca gcaaagtaac gccgttgtaa
      781 cgatttatca atccaaactc aaaaggcggc gagatgcgtt taactccgtg aaattaacaa
      841 accgccaaca acttgcaatt tgcaactacc gtttccaaga agaactcaac cacacaacgt
      901 atcctatccc aaaccacacg caactagtga cgcgtcataa ggacacgtgt cacaatttga
      961 ctggttaata atttcaccgc ttttgctata aattacctcc aatcccctta gcttcttcac
     1021 aattcagttc ccaaccctaa caattcttgt tcatatcgct tctctctact ttcaaggtat
     1081 gatccaattt ctctcttctt tctctgtaat cctttcgttg agttttgttt ccgatcaatc
     1141 ataggtagtt ttcttgtttc gaagcatgag atctaggaat tttttgtgat tttccaaaat
     1201 tgagatcggt ttgaaattga attttacagc ttgaatctca gatcttgttt tatcaatgtt
     1261 ttcgatggct tgcgatgtag atctatgata attgtggttc agttttgtta ggaatcgatt
     1321 tcggtttagc aattgcagat taattagggt ttccaattga attcttcaga tccgttatgg
     1381 aattatgtca aataatttat tcaaattgga aattattgtt agatccactc ttaatctgtt
     1441 tgatccaagc ttcaattagg gttttcactt gtttcaattt cttgttatgg attctgattt
     1501 atctgttgat gttagatcca ctcttaatct gtttgatcca agcgttaatt agggttttca
     1561 cttgtttcaa tttcgtgtgt tggattctga tttatctgtt gttgatgtga ttacagaatg
     1621 aagcaaggac aatcaagaaa aatagtactt ctaatagcat tcttagttct atgctgcttt
     1681 tcatcatttc ttctgggagg tatacttatt tttctttaat tttattctta ttttatgaag
     1741 ttttgtatat tagtttgtca tctttacaaa aacagtttgt aaggtatgca agttcactat
     1801 tacattattt ttgtataatt ctattttaaa taatttttac acacacatat atattcagtg
     1861 acggagtcag aaattttata gagcttgggc aaaaaaatta tcgcaaaatg ttactttctt
     1921 cctgggaata atttcacatt tcctgcagat aatttcacat ttcctacaga atctgcagga
     1981 aatcctaagc aaaatctgca ggaaatgtgt ttcctgcgga atttacataa aaatcagcat
     2041 aaaaaactaa aagtcctagt aatggctagg tttgctacaa agaaaattgg agctgagcct
     2101 gagctagtgt ccaggctagc tggggggtgg ctccgccctt gtatatattg tgtttgttgg
     2161 tttcttcatt ttatataaaa aaaacaaaca tttgtctaga agtcctaact caactggcaa
     2221 atgccgaaat tgcaagacct cgcattgtgt gtgagtttat ttttaaaaaa agaaaaaaca
     2281 acattttggc agttttgtaa ttaacgttca aatattgcag aagtggaaat tatttttaag
     2341 cttgtgtaac ctagttttat ttatttttat ttgtagcata tgcacgaaat ttgaggatag
     2401 actcatttga atttcatgca agacatacgg gtaatggagg agttaagcat gaagattatg
     2461 ttgtctccac tatggattat gcagcagtta agagaaaacc acctattcat aactaggctt
     2521 ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     2581 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     2641 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     2701 aaaatccagt actaaaatcc agatcgctaa gcttgaattc ttgcggagag cggagaatta
     2761 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact
     2821 gacagaaccg caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta
     2881 agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct
     2941 agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca
     3001 attagagtct catattcact ctcaaatgat tgaacaagat ggattgcacg caggttctcc
     3061 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3121 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3181 cctgtccggt gccctgaatg aactccaaga cgaggcagcg cggctatcgt ggctggccac
     3241 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3301 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3361 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3421 attcgaccac caagcgaaac atcgcatcga gcgaggacgt actcggatgg aagccggtct
     3481 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3541 caggctcaag gcgcggatgc ccgacggcga ggatctcgtc gtgacccagg gcgatgcctg
     3601 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3661 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3721 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3781 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcttgtcaag cagatcgttc
     3841 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     3901 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     3961 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     4021 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     4081 agatcgacgc tggatccgaa ttcggcaggg cccactgcat ccaccccagt acattaaaaa
     4141 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     4201 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     4261 aggcagccca tcagccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     4321 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     4381 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     4441 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     4501 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     4561 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     4621 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     4681 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     4741 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     4801 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     4861 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     4921 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     4981 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     5041 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     5101 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     5161 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     5221 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     5281 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     5341 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     5401 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     5461 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     5521 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     5581 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     5641 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     5701 cgccggcagc cggtgcgtcg tcgattagga agccgcccaa gggcgacgag caaccagatt
     5761 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     5821 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     5881 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     5941 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     6001 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     6061 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     6121 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     6181 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     6241 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     6301 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     6361 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     6421 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     6481 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     6541 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     6601 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaagcttct
     6661 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     6721 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     6781 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     6841 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     6901 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     6961 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     7021 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cgagctccca tccccccagg
     7081 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gccaattcgt
     7141 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7201 acaataacct tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag
     7261 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgcaaaag atacggaagg
     7321 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     7381 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     7441 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     7501 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     7561 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     7621 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     7681 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt
     7741 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     7801 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     7861 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     7921 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     7981 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     8041 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8101 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8161 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8221 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8281 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8341 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8401 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8461 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8521 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8581 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8641 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8701 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8761 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8821 tttacggttc ctgtggttgg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     8881 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
     8941 cctgtcaaac actgatagtt taaactccct cggtcacatg tgcatcctcg taaggaggac
     9001 gtcgttgtgg ttggtgcttt ccttacattc tgagcctctt tccttctaat ccactcatct
     9061 gcatcttctt gtgtccttac taatacctca ttggttccaa attccctccc tttaagcacc
     9121 agctcgtttc tgttcttcca cagcctccca agtatccaag ggactaaagc ctccacattc
     9181 ttcagatcag gatattcttg tttaagatgt tgaactctat ggaggtttgt atgaactgat
     9241 gatctaggac cggataagtt cccttcttca tagcgaactt attcaaagaa tgttttgtgt
     9301 atcattcttg ttacattgtt attaatgaaa aaatattatt ggtcattgga ctgaacacga
     9361 gtgttaaata tggaccaggc cccaaataag atccattgat atatgaatta aataacaaga
     9421 ataaatcgag tcaccaaacc acttgccttt tttaacgaga cttgttcacc aacttgatac
     9481 aaaagtcatt atcctatgca aatcaataat catacaaaaa tatccaataa cactaaaaaa
     9541 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     9601 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     9661 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     9721 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     9781 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     9841 gttaaggaaa ttgtggttgt cggacgaagt ccagtaataa acggcgtcaa agtggttgca
     9901 gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa
     9961 aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt
    10021 ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt
    10081 tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca attcagattt
    10141 caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt
    10201 tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta
    10261 tatgttcttt ggtttagatt ctgttaatct tagatcgtag acgattttct gggtttgatc
    10321 gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat
    10381 aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt
    10441 agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga ttaacagatg
    10501 cagatcttaa tggggtcatc caagaatgtt atcaaggagt tcatgaggtt taaggttcgc
    10561 atggaaggaa cggtcaatgg gcacgagttt gaaatagaag gcgaaggaga ggggaggcca
    10621 tacgaaggcc acaataccgt aaagcttaag gtaaccaagg ggggaccttt gccatttgct
    10681 tgggatattt tgtcaccaca atttcagtat ggaagcaagg tatatgtcaa gcaccctgcc
    10741 gacataccag actataaaaa gctgtcattt cctgaaggat ttaaatggga aagggtcatg
    10801 aactttgaag atggtggcgt cgttactgta acccaggatt ccagtttgca ggatggctgt
    10861 ttcatctaca aggtcaagtt cattggcgtg aactttcctt ccgatggacc tgttatgcaa
    10921 aagaaaacaa tgggctggga agccagcact gagcgtttgt atcctcgtga tggcgtgttg
    10981 aaag
//
