LOCUS       sequence-325447-       10358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtPSY6:T35s
FEATURES             Location/Qualifiers
     source          1..10358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    86..260
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        360..539
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             561..1355
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(615..634)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1225..1244
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1368..1620
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1715..1739
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2003..2632
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3061..4134
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4200..4394
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        4677..4781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4782..5576
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5747..6335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6236..6255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6458..6482
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      8735..8987
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gattatgcac catctggggc aaatggtcgt cacactccaa gagcaccgta agcttctcta
       61 gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
      121 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
      181 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
      241 ccagtactaa aatccagatc gctaagcttg aattcttgcg gagagcggag aattaaggga
      301 gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag
      361 aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac
      421 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa
      481 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
      541 agtctcatat tcactctcaa atgattgaac aagatggatt gcacgcaggt tctccggccg
      601 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      661 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      721 ccggtgccct gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg
      781 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      841 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      901 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      961 accaccaagc gaaacatcgc atcgagcgag gacgtactcg gatggaagcc ggtcttgtcg
     1021 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     1081 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccagggcgat gcctgcttgc
     1141 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     1201 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     1261 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     1321 tcgccttcta tcgccttctt gacgagttct tctgagcttg tcaagcagat cgttcaaaca
     1381 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     1441 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     1501 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     1561 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     1621 gacgctggat ccgaattcgg cagggcccac tgcatccacc ccagtacatt aaaaacgtcc
     1681 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     1741 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     1801 gcccatcagc cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     1861 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     1921 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     1981 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     2041 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     2101 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     2161 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     2221 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     2281 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     2341 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     2401 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     2461 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     2521 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     2581 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     2641 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     2701 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     2761 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     2821 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     2881 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     2941 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     3001 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     3061 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     3121 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     3181 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     3241 gcagccggtg cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     3301 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     3361 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     3421 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     3481 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     3541 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     3601 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     3661 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     3721 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     3781 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     3841 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     3901 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     3961 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     4021 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     4081 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     4141 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct
     4201 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     4261 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     4321 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     4381 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     4441 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     4501 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     4561 tcgccactcg accgccggcg cccacatcaa ggcaccgagc tcccatcccc ccaggggctg
     4621 cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggccaa ttcgtgcgcg
     4681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4741 aaccttgata aatgcttcaa taatattgaa aaaggaagag tatggctaaa atgagaatat
     4801 caccggaatt gaaaaaactg atcgaaaaat accgctgcgc aaaagatacg gaaggaatgt
     4861 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
     4921 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
     4981 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
     5041 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
     5101 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
     5161 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
     5221 tgaataacga tctggccgat gtggattgcg aaaactggga agaggacact ccatttaaag
     5281 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
     5341 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
     5401 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
     5461 cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
     5521 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagctgt
     5581 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5641 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5881 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6121 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6361 ggttcctgtg gttggcacat acaaatggac gaacggataa accttttcac gcccttttaa
     6421 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
     6481 caaacactga tagtttaaac tccctcggtc acatgtgcat cctcgtaagg aggacgtcgt
     6541 tgtggttggt gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
     6601 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
     6661 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
     6721 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
     6781 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
     6841 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
     6901 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
     6961 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
     7021 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaaattaa
     7081 aagaaatgga taatttcaca atatgttata cgataaagaa gttacttttc caagaaattc
     7141 actgatttta taagcccact tgcattagat aaatggcaaa aaaaaacaaa aaggaaaaga
     7201 aataaagcac gaagaattct agaaaatacg aaatacgctt caatgcagtg ggacccacgg
     7261 ttcaattatt gccaattttc agctccaccg tatatttaaa aaataaaacg ataatgctaa
     7321 aaaaatataa atcgtaacga tcgttaaatc tcaacggctg gatcttatga cgaccgttaa
     7381 ggaaattgtg gttgtcggac gaagtccagt aataaacggc gtcaaagtgg ttgcagccgg
     7441 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     7501 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     7561 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     7621 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     7681 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     7741 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     7801 tctttggttt agattctgtt aatcttagat cgtagacgat tttctgggtt tgatcgttag
     7861 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     7921 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     7981 ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agatgcagat
     8041 cttaatgggg tcatccaaga atgttatcaa ggagttcatg aggtttaagg ttcgcatgga
     8101 aggaacggtc aatgggcacg agtttgaaat agaaggcgaa ggagagggga ggccatacga
     8161 aggccacaat accgtaaagc ttaaggtaac caagggggga cctttgccat ttgcttggga
     8221 tattttgtca ccacaatttc agtatggaag caaggtatat gtcaagcacc ctgccgacat
     8281 accagactat aaaaagctgt catttcctga aggatttaaa tgggaaaggg tcatgaactt
     8341 tgaagatggt ggcgtcgtta ctgtaaccca ggattccagt ttgcaggatg gctgtttcat
     8401 ctacaaggtc aagttcattg gcgtgaactt tccttccgat ggacctgtta tgcaaaagaa
     8461 aacaatgggc tgggaagcca gcactgagcg tttgtatcct cgtgatggcg tgttgaaagg
     8521 agagattcat aaggctctga agctgaaaga cggtggtcat tacctagttg aattcaaaag
     8581 tatttacatg gcaaagaagc ctgtgcagct accagggtac tactatgttg actccaaact
     8641 ggatataaca agccacaacg aagattatac aatcgttgag cagtatgaaa gaaccgaggg
     8701 acgccaccat ctgttccttt aagcttgtca agcagatcgt tcaaacattt ggcaataaag
     8761 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     8821 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     8881 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     8941 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac gctgagctcg
     9001 aattctagtg gagggagaga ggattttgag gaaataatta attgaattac ttgtattatt
     9061 gataaagtaa tttagataag ttgttagtac aacttattgc aactatggat agacaaaaat
     9121 cacttatttt aaggggagaa gtatataaca acttattata aaatttctga gcagccggcc
     9181 tcctccaatc aatcatagag agtggagccc attctggaaa accaaggaac ccccaacttg
     9241 cactggtgcg gtggcccaat tcaaaaaaaa cggggcccag caaagtaacg ccgttgtaac
     9301 gatttatcaa tccaaactca aaaggcggcg agatgcgttt aactccgtga aattaacaaa
     9361 ccgccaacaa cttgcaattt gcaactaccg tttccaagaa gaactcaacc acacaacgta
     9421 tcctatccca aaccacacgc aactagtgac gcgtcataag gacacgtgtc acaatttgac
     9481 tggttaataa tttcaccgct tttgctataa attacctcca atccccttag cttcttcaca
     9541 attcagttcc caaccctaac aattcttgtt catatcgctt ctctctactt tcaaggtatg
     9601 atccaatttc tctcttcttt ctctgtaatc ctttcgttga gttttgtttc cgatcaatca
     9661 taggtagttt tcttgtttcg aagcatgaga tctaggaatt ttttgtgatt ttccaaaatt
     9721 gagatcggtt tgaaattgaa ttttacagct tgaatctcag atcttgtttt atcaatgttt
     9781 tcgatggctt gcgatgtaga tctatgataa ttgtggttca gttttgttag gaatcgattt
     9841 cggtttagca attgcagatt aattagggtt tccaattgaa ttcttcagat ccgttatgga
     9901 attatgtcaa ataatttatt caaattggaa attattgtta gatccactct taatctgttt
     9961 gatccaagct tcaattaggg ttttcacttg tttcaatttc ttgttatgga ttctgattta
    10021 tctgttgatg ttagatccac tcttaatctg tttgatccaa gcgttaatta gggttttcac
    10081 ttgtttcaat ttcgtgtgtt ggattctgat ttatctgttg ttgatgtgat tacagaatgg
    10141 caggatcttt ctttagttta ttcgtgattc ttcttcttgt tctttctcat cttttatgct
    10201 tgacagcagt cccatttaca agaactgaaa accttatgca agatgtccaa gttcacctaa
    10261 ttactctgga aaatacccct aaggttatta ctgagaaaaa catgcatttg gaagagccaa
    10321 agccaagaat taatgagagg atggatttag aactcaat
//
