LOCUS       sequence-325449-       12691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Roy S, Torres-Jerez I, Zhang S, Liu W, Schiessl K, Jain D, Boschiero
            C, Lee HK, Krom N, Zhao PX, Murray JD, Oldroyd GED, Scheible WR, 
            Udvardi M
  TITLE     The peptide GOLVEN10 alters root development and noduletaxis in 
            Medicago truncatula.
  JOURNAL   Plant J. 2024 May;118(3):607-625. doi: 10.1111/tpj.16626. Epub 2024 
            Feb 15.
  PUBMED    38361340
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16626";
            journalName: "Plant J"; date: "2024-05"; volume: "118"; issue: "3"; 
            pages: "607-625"
COMMENT     Sequence Label: LjUBQ:MtGLV6:T35s
FEATURES             Location/Qualifiers
     source          1..12691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1333..1962
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2391..3464
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3530..3724
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3960..3982)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4068..4208
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4123..4142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4223..4240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4394..4982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4474..4493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5228..6019)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6540..6564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6657..6909)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7107..8132)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      complement(8448..8700)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(8713..9387,9388..9390,9391..9393))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MARSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(8775..8798)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(8973..8992)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9190..9210
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     misc_feature    join(12669..12691,1..2)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
       61 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
      121 ctttaaagta ctttaaagta ctttaaagta ctttgatcca acccctccgc tgctatagtg
      181 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
      241 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
      301 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
      361 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
      421 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
      481 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
      541 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
      601 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
      661 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
      721 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
      781 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
      841 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
      901 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
      961 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     1021 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     1081 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     1141 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     1201 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     1261 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     1321 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     1381 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     1441 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     1501 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     1561 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     1621 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     1681 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     1741 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     1801 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     1861 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     1921 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     1981 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     2041 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     2101 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     2161 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     2221 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     2281 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     2341 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     2401 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     2461 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     2521 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     2581 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     2641 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     2701 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     2761 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     2821 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     2881 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     2941 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     3001 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     3061 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     3121 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     3181 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     3241 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     3301 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     3361 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     3421 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     3481 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     3541 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     3601 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     3661 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     3721 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     3781 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     3841 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     3901 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3961 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4021 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     4081 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     4141 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4201 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4801 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5041 aagggatttt ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct
     5101 taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt
     5161 gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaatttctag
     5221 ctagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     5281 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     5341 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     5401 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     5461 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     5521 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     5581 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     5641 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     5701 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     5761 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     5821 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     5881 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     5941 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     6001 cgatcaccgc ttcccccatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
     6061 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     6121 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
     6181 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     6241 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgcccgaa
     6301 ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
     6361 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
     6421 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     6481 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     6541 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
     6601 acgtttttaa tgtactgaat taacgccgaa ttgaattatc agcttgcatg ccggtcgatc
     6661 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     6721 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     6781 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     6841 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     6901 tgaacgatct gcttgactct agggaattaa ttcctgaatc actgcgaccg gccctcccgc
     6961 gacccagccg agcgagctta gcgaactgtg gacgagaact gtgccaccaa gcgtaaggcc
     7021 gttctctcgc atttgccttg ctaggctcgc gcgagttgct ggctgaggcg ttctcgaaat
     7081 cagctcttgt tcggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt
     7141 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     7201 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     7261 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     7321 agaccaatgc ggagcatata cgcccggagc cttggcgatc ctgcaagctc cggatgcctc
     7381 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     7441 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     7501 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     7561 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     7621 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     7681 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     7741 ctcgtctggc taagatcggc cgcagcgata gcatccatag cctccgcgac cggctgaaga
     7801 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     7861 atgcaatagg tcaggctctc gctgaactcc ccaatgtcaa gcacttccgg aatcgggagc
     7921 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     7981 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     8041 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     8101 acagacgtcg cggtgagttc aggctttttc atagggggga ttcgagttga gagtgaatat
     8161 gagactctaa ttggataccg aatttatgga acgtcagtgg agcatttttg acaagaaata
     8221 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
     8281 tgtgcttagc tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt
     8341 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
     8401 ctcccttaat tctccgctca tgatcgataa ttcgtcgacg gcgcgccgat ctagtaacat
     8461 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     8521 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     8581 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     8641 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     8701 gggaattgga tcctacagga acaggtggtg gcggccctcg gtgcgctcgt actgctccac
     8761 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gagtccacgt agtagtagcc
     8821 gggcagctgc acgggcttct tggccatgta gatggacttg aactccacca ggtagtggcc
     8881 gccgtccttc agcttcaggg ccttgtggat ctcgcccttc agcacgccgt cgcgggggta
     8941 caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga
     9001 ggggaagttc acgccgatga acttcacctt gtagatgaag cagccgtcct gcagggagga
     9061 gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
     9121 gccctcgggg aaggacagct tcttgtagtc ggggatgtcg gcggggtgct tcacgtacac
     9181 cttggagccg tactggaact ggggggacag gatgtcccag gcgaagggca gggggccgcc
     9241 cttggtcacc ttcagcttca cggtgttgtg gccctcgtag gggcggccct cgccctcgcc
     9301 ctcgatctcg aactcgtggc cgttcacggt gccctccatg cgcaccttga agcgcatgaa
     9361 ctccttgatg acgttcttgg aggagcgcgc catggcaaag atctgcatct gttaatcaga
     9421 aaaactcaga ttaatcgaca aattcgatcg cacaaactag aaactaacac cagatctaga
     9481 tagaaatcac aaatcgaaga gtaattattc gacaaaactc aaattatttg aacaaatcgg
     9541 atgatatcta tgaaacccta atcgagaatt aagatgatat ctaacgatca aacccagaaa
     9601 atcgtcttcg atctaagatt aacagaatct aaaccaaaga acatatacga aattgggatc
     9661 gaacgaaaac aaaatcgaag attttgagag aataaggaac acagaaattt accttgatca
     9721 cggtagagag aattgagaga aagtttttaa gattttgaga aattgaaatc tgaattgtga
     9781 agaagaagag ctctttgggt attgttttat agaagaagaa gaagaaaaga cgaggacgac
     9841 taggtcacga gaaagctaag gcggtgaagc aatagctaat aataaaatga cacgtgtatt
     9901 gagcgttgtt tacacgcaaa gttgtttttg gctaattgcc ttatttttag gttgaggaaa
     9961 agtatttgtg ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa ccactttgac
    10021 gccgtttatt actgactcgt cgacaaccac aatttctaac ggtcgtcata agatccagcc
    10081 gttgagattt aacgatcgtt acgatttata tttttttagc attatcgttt tattttttaa
    10141 atatacggtg gagctgaaaa ttggcaataa ttgaaccgtg ggtcccactg cattgaagcg
    10201 tatttcgtat cttctagaat tcttcgtgct ttatttcttt tcctttttgt ttttttttgc
    10261 catttatcta atgcaagtgg gcttataaaa tcagtgaatt tcttggaaaa gtaacttctt
    10321 tatcgtataa catattgtga aattatccat ttcttttaat tttttagtgt tattggatat
    10381 ttttgtatga ttattgattt gcataggata atgacttttg tatcaagttg gtgaacaagt
    10441 ctcgttaaaa aaggcaagtg gtttggtgac tcgatttatt cttgttattt aattcatata
    10501 tcaatggatc ttatttgggg cctggtccat atttaacact cgtgttcagt ccaatgacca
    10561 ataatatttt ttcattaata acaatgtaac aagaatgata cacaaaacat tctttgaata
    10621 agttcgctat gaagaaggga acttatccgg tcctagatca tcagttcata caaacctcca
    10681 tagagttcaa catcttaaac aagaatatcc tgatctgaag aatgtggagg ctttagtccc
    10741 ttggatactt gggaggctgt ggaagaacag aaacgagctg gtgcttaaag ggagggaatt
    10801 tggaaccaat gaggtattag taaggacaca agaagatgca gatgagtgga ttagaaggaa
    10861 agaggctcag aatgtaagga aagcaccaac cacaacggcg cgcccgggag agaggatttt
    10921 gaggaaataa ttaattgaat tacttgtatt attgataaag taatttagat aagttgttag
    10981 tacaacttat tgcaactatg gatagacaaa aatcacttat tttaagggga gaagtatata
    11041 acaacttatt ataaaatttc tgagcagccg gcctcctcca atcaatcata gagagtggag
    11101 cccattctgg aaaaccaagg aacccccaac ttgcactggt gcggtggccc aattcaaaaa
    11161 aaacggggcc cagcaaagta acgccgttgt aacgatttat caatccaaac tcaaaaggcg
    11221 gcgagatgcg tttaactccg tgaaattaac aaaccgccaa caacttgcaa tttgcaacta
    11281 ccgtttccaa gaagaactca accacacaac gtatcctatc ccaaaccaca cgcaactagt
    11341 gacgcgtcat aaggacacgt gtcacaattt gactggttaa taatttcacc gcttttgcta
    11401 taaattacct ccaatcccct tagcttcttc acaattcagt tcccaaccct aacaattctt
    11461 gttcatatcg cttctctcta ctttcaaggt atgatccaat ttctctcttc tttctctgta
    11521 atcctttcgt tgagttttgt ttccgatcaa tcataggtag ttttcttgtt tcgaagcatg
    11581 agatctagga attttttgtg attttccaaa attgagatcg gtttgaaatt gaattttaca
    11641 gcttgaatct cagatcttgt tttatcaatg ttttcgatgg cttgcgatgt agatctatga
    11701 taattgtggt tcagttttgt taggaatcga tttcggttta gcaattgcag attaattagg
    11761 gtttccaatt gaattcttca gatccgttat ggaattatgt caaataattt attcaaattg
    11821 gaaattattg ttagatccac tcttaatctg tttgatccaa gcttcaatta gggttttcac
    11881 ttgtttcaat ttcttgttat ggattctgat ttatctgttg atgttagatc cactcttaat
    11941 ctgtttgatc caagcgttaa ttagggtttt cacttgtttc aatttcgtgt gttggattct
    12001 gatttatctg ttgttgatgt gattacagat ggttgcttca caaaacaaac gcattctaca
    12061 agtgattttt gttctccttt gcttcttctc catccatatt caagttaaag gtaccttttg
    12121 cttcaaatat tacaacattg gattaagaat attcccattt tttttatgtt taattaactt
    12181 ttcttacatt ttttttgtta tgcgcagcaa gagtattgaa agaaataaac aaccaaagta
    12241 gtgagggtgt caacaaagct caacaacata aggaagtaca tgctgacatg tttaagctaa
    12301 aagaaggagg agataacgtt ggttctgatg ttttcaccat ggattatact ccagcatcaa
    12361 ggaaaccacc aattcacaat taacggccat gctagagtcc gcaaaaatca ccagtctctc
    12421 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
    12481 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
    12541 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
    12601 cagtgaaagc ttagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
    12661 tcagtgtttg acaggatata ttggcgggta a
//
