LOCUS       sequence-32545-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST8_FLAGHA_FUS_HIS
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    78..102
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             144..170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             578..589
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc
       61 atcgggcgcg gatcatcaca agtttgtaca aaaaagcagg ctccaccatg aatcccatgg
      121 actacaagga cgacgatgac aagtaccctt atgacgtgcc cgattacgct ggatccgaat
      181 tcgagctccg tcgacccatg gcctcaaacg attataccca acaagcaacc caaagctatg
      241 gggcctaccc cacccagccc gggcagggct attcccagca gagcagtcag ccctacggac
      301 agcagagtta cagtggttat agccagtcca cggacacttc aggatatggc cagagcagct
      361 attcttctta tggccagagc cagaacacag gctatggaac tcagtcaact ccccagggat
      421 atggctcgac tggcggctat ggcagtagcc agagctccca atcgtcttac gggcagcagt
      481 cctcctaccc tggctatggc cagcagccag ctcccagcag cacctcggga agttacggta
      541 gcagttctca gagcagcagc tatgggcagc cccagagtgg gagctacagc cagcagccta
      601 gctatggtgg acagcagcaa agctatggac agcagcaaag ctataatccc cctcagggct
      661 atggacagca gaaccagtac aacagcagca gtggtggtgg aggtggaggt ggaggtggag
      721 gtaactatgg ccaagatcaa tcctccatga gtagtggtgg tggcagtggt ggcggttatg
      781 gcaatcaaga ccagagtggt ggaggtggca gcggtggcta tggacagcag gaccgtggag
      841 gccgcggcag gggtggcagt ggtggcggcg gcggcggcgg cggtggtggt tacaccgcag
      901 cagtggtggc tatgaaccca gaggtcgtg
//
