LOCUS       sequence-325452-       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtPIP7:T35s
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      554..806
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    2209..2383
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2483..2662
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2684..3478
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2738..2757)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3348..3367
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      3491..3743
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3838..3862
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4126..4755
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5184..6257
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6323..6517
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        6800..6904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6905..7699
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7870..8458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8359..8378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8581..8605
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taaggtaacc aaggggggac ctttgccatt tgcttgggat attttgtcac cacaatttca
       61 gtatggaagc aaggtatatg tcaagcaccc tgccgacata ccagactata aaaagctgtc
      121 atttcctgaa ggatttaaat gggaaagggt catgaacttt gaagatggtg gcgtcgttac
      181 tgtaacccag gattccagtt tgcaggatgg ctgtttcatc tacaaggtca agttcattgg
      241 cgtgaacttt ccttccgatg gacctgttat gcaaaagaaa acaatgggct gggaagccag
      301 cactgagcgt ttgtatcctc gtgatggcgt gttgaaagga gagattcata aggctctgaa
      361 gctgaaagac ggtggtcatt acctagttga attcaaaagt atttacatgg caaagaagcc
      421 tgtgcagcta ccagggtact actatgttga ctccaaactg gatataacaa gccacaacga
      481 agattataca atcgttgagc agtatgaaag aaccgaggga cgccaccatc tgttccttta
      541 agcttgtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
      601 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
      661 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
      721 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
      781 cgcggtgtca tctatgttac tagatcgacg ctgagctcga attctagtgg agggagagag
      841 gattttgagg aaataattaa ttgaattact tgtattattg ataaagtaat ttagataagt
      901 tgttagtaca acttattgca actatggata gacaaaaatc acttatttta aggggagaag
      961 tatataacaa cttattataa aatttctgag cagccggcct cctccaatca atcatagaga
     1021 gtggagccca ttctggaaaa ccaaggaacc cccaacttgc actggtgcgg tggcccaatt
     1081 caaaaaaaac ggggcccagc aaagtaacgc cgttgtaacg atttatcaat ccaaactcaa
     1141 aaggcggcga gatgcgttta actccgtgaa attaacaaac cgccaacaac ttgcaatttg
     1201 caactaccgt ttccaagaag aactcaacca cacaacgtat cctatcccaa accacacgca
     1261 actagtgacg cgtcataagg acacgtgtca caatttgact ggttaataat ttcaccgctt
     1321 ttgctataaa ttacctccaa tccccttagc ttcttcacaa ttcagttccc aaccctaaca
     1381 attcttgttc atatcgcttc tctctacttt caaggtatga tccaatttct ctcttctttc
     1441 tctgtaatcc tttcgttgag ttttgtttcc gatcaatcat aggtagtttt cttgtttcga
     1501 agcatgagat ctaggaattt tttgtgattt tccaaaattg agatcggttt gaaattgaat
     1561 tttacagctt gaatctcaga tcttgtttta tcaatgtttt cgatggcttg cgatgtagat
     1621 ctatgataat tgtggttcag ttttgttagg aatcgatttc ggtttagcaa ttgcagatta
     1681 attagggttt ccaattgaat tcttcagatc cgttatggaa ttatgtcaaa taatttattc
     1741 aaattggaaa ttattgttag atccactctt aatctgtttg atccaagctt caattagggt
     1801 tttcacttgt ttcaatttct tgttatggat tctgatttat ctgttgatgt tagatccact
     1861 cttaatctgt ttgatccaag cgttaattag ggttttcact tgtttcaatt tcgtgtgttg
     1921 gattctgatt tatctgttgt tgatgtgatt acagaatgaa gaaaagtata ttgattgtgt
     1981 tttttatcat tatggttgtg atgtcatcat cacagttatg ttttgttcaa agcagagttc
     2041 ttcaatccaa ggagtttaga aaagatggag tgacatcttt tgtttcttct aacaattcaa
     2101 gcaatcgtgg ttcaaaggaa agcttttcat tcatactagc ttctggacca agcaaaaaag
     2161 gccctggaca ttgagcttct ctagctagag tcgatcgaca agctcgagtt tctccataat
     2221 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
     2281 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     2341 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcgctaagc ttgaattctt
     2401 gcggagagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc
     2461 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag ccgcgggttt
     2521 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
     2581 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
     2641 aattcccctc ggtatccaat tagagtctca tattcactct caaatgattg aacaagatgg
     2701 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     2761 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     2821 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg
     2881 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     2941 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3001 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3061 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gaggacgtac
     3121 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3181 gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt
     3241 gacccagggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3301 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3361 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3421 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     3481 ttgtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     3541 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     3601 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     3661 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     3721 ggtgtcatct atgttactag atcgacgctg gatccgaatt cggcagggcc cactgcatcc
     3781 accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     3841 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     3901 aaaatcacca ctcgatacag gcagcccatc agccgtgcgg ctgcatgaaa tcctggccgg
     3961 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4021 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4081 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4141 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     4201 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4261 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     4321 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     4381 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     4441 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     4501 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     4561 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     4621 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     4681 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     4741 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     4801 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     4861 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     4921 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     4981 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5041 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5101 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     5161 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     5221 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     5281 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     5341 gcaaagaatc ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag ccgcccaagg
     5401 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     5461 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     5521 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     5581 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     5641 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     5701 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     5761 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     5821 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     5881 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     5941 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6001 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6061 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6121 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6181 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6241 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     6301 aaggtcgaaa aagcttcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     6361 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     6421 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     6481 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     6541 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     6601 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     6661 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg
     6721 agctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg
     6781 cagcgctggc caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6841 tatgtatccg ctcatgagac aataaccttg ataaatgctt caataatatt gaaaaaggaa
     6901 gagtatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     6961 cgcaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     7021 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     7081 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     7141 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     7201 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     7261 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     7321 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     7381 ggaagaggac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     7441 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     7501 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     7561 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     7621 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     7681 actggatgaa ttgttttagc tgtcagacca agtttactca tatatacttt agattgattt
     7741 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7801 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7861 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7921 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7981 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     8041 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8101 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8161 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8221 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8281 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8341 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8401 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8461 cgccagcaac gcggcctttt tacggttcct gtggttggca catacaaatg gacgaacgga
     8521 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     8581 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactccctcg gtcacatgtg
     8641 catcctcgta aggaggacgt cgttgtggtt ggtgctttcc ttacattctg agcctctttc
     8701 cttctaatcc actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat
     8761 tccctccctt taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg
     8821 actaaagcct ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg
     8881 aggtttgtat gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat
     8941 tcaaagaatg ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg
     9001 tcattggact gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat
     9061 atgaattaaa taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact
     9121 tgttcaccaa cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata
     9181 tccaataaca ctaaaaaaat taaaagaaat ggataatttc acaatatgtt atacgataaa
     9241 gaagttactt ttccaagaaa ttcactgatt ttataagccc acttgcatta gataaatggc
     9301 aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat tctagaaaat acgaaatacg
     9361 cttcaatgca gtgggaccca cggttcaatt attgccaatt ttcagctcca ccgtatattt
     9421 aaaaaataaa acgataatgc taaaaaaata taaatcgtaa cgatcgttaa atctcaacgg
     9481 ctggatctta tgacgaccgt taaggaaatt gtggttgtcg gacgaagtcc agtaataaac
     9541 ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat
     9601 acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg
     9661 ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac
     9721 ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct
     9781 tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta
     9841 ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg
     9901 ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta gatcgtagac
     9961 gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat
    10021 catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt
    10081 ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt
    10141 ttttctgatt aacagatgca gatcttaatg gggtcatcca agaatgttat caaggagttc
    10201 atgaggttta aggttcgcat ggaaggaacg gtcaatgggc acgagtttga aatagaaggc
    10261 gaaggagagg ggaggccata cgaaggccac aataccgtaa agct
//
