LOCUS       sequence-32546-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_TO_EWSR1
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             183..206
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             207..233
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    249..273
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tcctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacca
      181 tggactacaa ggacgacgat gacaagtacc cttatgacgt gcccgattac gctttgaacc
      241 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccaat tcagtcgaca
      301 tggcgtccac ggattacagt acctatagcc aagctgcagc gcagcagggc tacagtgctt
      361 acaccgccca gcccactcaa ggatatgcac agaccaccca ggcatatggg caacaaagct
      421 atggaaccta tggacagccc actgatgtca gctataccca ggctcagacc actgcaacct
      481 atgggcagac cgcctatgca acttcttatg gacagcctcc cactggttat actactccaa
      541 ctgcccccca ggcatacagc cagcctgtcc aggggtatgg cactggtgct tatgatacca
      601 ccactgctac agtcaccacc acccaggcct cctatgcagc tcagtctgca tatggcactc
      661 agcctgctta tccagcctat gggcagcagc cagcagccac tgcacctaca agaccgcagg
      721 atggaaacaa gcccactgag actagtcaac ctcaatctag cacagggggt tacaaccagc
      781 ccagcctagg atatggacag agtaactaca gttatcccca ggtacctggg agctacccca
      841 tgcagccagt cactgcacct ccatcctacc ctcctaccag ctattcctct acacagccga
      901 ctagttatga tcagagcagt tactctcagc agaca
//
