LOCUS       sequence-325468-        5927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct-expressing oNigri AAV Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5927)
  TITLE     Enhancer AAVs from the Allen Institute
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA
FEATURES             Location/Qualifiers
     source          1..5927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1484..2072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1537..1557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1955..1966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2104..2580
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2303..2322)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2620..2760
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2620..2749
                     /label=AAV2 ITR
     rep_origin      2835..3290
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2922..2941)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3132..3153
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3307..3326)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3426..3448
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3486..3504)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3572..3676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3677..3745,3746..4537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3895..3914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4708..5296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5197..5216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5358..5487
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        join(5523..5927,1..43)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagggtaccg ccgccaccat
       61 gaagaagaag aggaaggtgg acaagcacca cctgctgtct acccggacca cagctcctac
      121 agacggcagc gatttcaact ccgataacgt gaccaagaac caccacgcca tcctgatcga
      181 gagggccctg gccagcgccc tggccagacc tggcctgctg ctggagacac actttcagcc
      241 aatctaccag agggctgaca ggctgggagt ggacctgtct gattgcccca gcttcttcgt
      301 ggctacccag aggctgctgg cccagcacca ccagcacgga gctggactgt ctccacgcag
      361 cctggagcag ttccacagcg ccgtgagggt gtttacacag tggtgtcacg tgaacaggag
      421 aaccgctctg ccagcctccg ctgacacatt cgtgctgttt gctagaagca acgcccccca
      481 catcaccctg tccacactgc acatctacgt gtgggccatc cggaagctgc acctgatcac
      541 cggcctggtg gacccaacag attctcaggc tgtgaagcag cacctgagca gaatcaagaa
      601 gcagaagatc gcccagttcg acaccgctca ggatcaggcc gtgcctctgt ccgacgagga
      661 ttaccgcacc gctatgacac tgctgatggc cgacgatcac ccaatctctt ggagggacgc
      721 taccctgctg ggactggcct accacacaat gctgcggcag agcgagctgg tgcgcatcca
      781 gctgacccac atccagccac gctccgacgg cgattggaca ctggagatcc cttacaccaa
      841 gacaaacaag accggaaggt ctgagtacgt gacactgccc cactacctga tgcctatcct
      901 gcagagatac ctgagcctgt gcggcgaccg caccctgaca gatccaggat acctgttcgt
      961 gccactgacc aggagcggcg tgccccggcg ccagagaatg cacgtgacct ccacacccag
     1021 ggccccttct ggagtgaccc ctaggagaaa gacaagaggc ggaatccagt ttgctccagt
     1081 ggctccagac gctgtgaccg attccatcgc tgctccacct gaccctgctc caatctctga
     1141 ggaggtgcag cctgtggctc caaagctggt ggcccgcaca ctgaagaaga tcggcgagag
     1201 gctggccacc cacacaagct ccccacaggc tgatagggct tactccggac actctgctag
     1261 ggtgggaaga gccatccacc tgctggagat cggcgctaga aaggaggaca tcgtgaaggc
     1321 cggacggtgg aagtccgaca tcatgtttga gcggtacacc cgccagtacg acgtgaacga
     1381 tggctacctg gctcagatca ggcaggctga ggacagacac tggcacgctc agaccgagcc
     1441 accagattct acaggatgag aattcgatat caagcttatc gataatcaac ctctggatta
     1501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     1561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     1621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     1681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     1741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     1801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1861 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     1921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     1981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2041 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     2101 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2161 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     2221 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     2281 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     2341 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     2401 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     2461 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     2521 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     2581 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     2641 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     2701 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     2761 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     2821 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2881 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     2941 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3001 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     3061 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3121 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     3181 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     3241 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     3301 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3361 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3421 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3481 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3541 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3601 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3661 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3721 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3781 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3841 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3901 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3961 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4021 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4081 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4141 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4201 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4261 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4321 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4381 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4441 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4501 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4561 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4621 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4681 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4741 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4801 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4861 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4921 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4981 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5041 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5101 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5161 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5221 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5281 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5341 gccttttgct cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     5401 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     5461 tggccaactc catcactagg ggttcctgcg gccgcacgcg tctgcagagg gccctgcgta
     5521 tgagtgcaag tgggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc
     5581 gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca
     5641 gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg
     5701 cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc
     5761 gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc
     5821 cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg
     5881 cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtc
//
