LOCUS       sequence-325469-       10893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector expressing plant-codon-optimized Cas9 under UBQ10
            promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat 
            specific red fluorescence for screening trangene free plants;.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, 
            Schwab R, Weigel D
  TITLE     An efficient CRISPR vector toolbox for engineering large deletions 
            in Arabidopsis thaliana.
  JOURNAL   Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. 
            eCollection 2018.
  PUBMED    30083222
REFERENCE   2  (bases 1 to 10893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-08-2"; volume: "14:65. doi"; pages: " 
            10.1186/s13007-018-0330-7. eCollection 2018"
COMMENT     Sequence Label: pRWJB004
FEATURES             Location/Qualifiers
     source          1..10893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..436
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    563..585
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             1267..1314
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1321..1341
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1366..2802,2992..5655)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2803..2991
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5656..5703
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5709..6350
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(6573..6896)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     6806..6822
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6892..6908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6892..6908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6905..6927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6916..6932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6940..6946,6947..6964,6965..6970))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6985..7006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7123..7140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7519..7771)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(8055..8074)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8324..8342
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8941..8965
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9056..9644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9136..9155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9815..10603)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt ggatagaggg
       61 tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg gctttccggc
      121 ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga
      181 gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg
      241 ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa gtagttacag
      301 caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta ttggtcgccc
      361 ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt
      421 gcccaccgtc gagcgccttt gcccacaacc cggcggccgg ccgcaacaga tcgttttata
      481 aatttttttt tttgaaaaag aaaaagcccg aaaggcggca acctctcggg cttctggatt
      541 tccgatcccc ggaattagat cttggcagga tatattgtgg tgtaacgttc tcgaactagg
      601 ctcggacgaa gtaagcggcc gccgacgagt cagtaataaa cggcgtcaaa gtggttgcag
      661 ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa
      721 ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt
      781 tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt
      841 cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc
      901 aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt
      961 ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat
     1021 atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg
     1081 ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata
     1141 atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta
     1201 gtttctagtt tgtgcgatcg aatttgtaga ttaatctgag tttttctgat taacagcgga
     1261 tccatggatt acaaggatga tgatgataag gattacaagg atgatgatga taagatggct
     1321 ccaaagaaga agagaaaggt tggaatccac ggagttccag ctgctgataa gaagtactct
     1381 atcggacttg acatcggaac caactctgtt ggatgggctg ttatcaccga tgagtacaag
     1441 gttccatcta agaagttcaa ggttcttgga aacaccgata gacactctat caagaagaac
     1501 cttatcggtg ctcttctttt cgattctgga gaaaccgctg aggctaccag attgaagaga
     1561 accgctagaa gaagatacac cagaagaaag aacagaatct gctaccttca ggaaatcttc
     1621 tctaacgaga tggctaaggt tgatgattct ttcttccaca gacttgagga gtctttcctt
     1681 gttgaggagg ataagaagca cgagagacac ccaatcttcg gaaacatcgt tgatgaggtt
     1741 gcttaccacg agaagtaccc aaccatctac caccttagaa agaagttggt tgattctacc
     1801 gataaggctg atcttagact tatctacctt gctcttgctc acatgatcaa gttcagagga
     1861 cacttcctta tcgagggtga ccttaaccca gataactctg atgttgataa gttgttcatc
     1921 cagcttgttc agacctacaa ccagcttttc gaggagaacc caatcaacgc ttctggagtt
     1981 gatgctaagg ctatcctttc tgctagactt tctaagtctc gtagacttga gaaccttatc
     2041 gctcagcttc caggagagaa gaagaacgga cttttcggaa accttatcgc tctttctctt
     2101 ggacttaccc caaacttcaa gtctaacttc gatcttgctg aggatgctaa gttgcagctt
     2161 tctaaggata cctacgatga tgatcttgat aaccttcttg ctcagatcgg agatcagtac
     2221 gctgatcttt tccttgctgc taagaacctt tctgatgcta tccttctttc tgacatcctt
     2281 agagttaaca ccgagatcac caaggctcca ctttctgctt ctatgatcaa gagatacgat
     2341 gagcaccacc aggatcttac ccttttgaag gctcttgtta gacagcagct tccagagaag
     2401 tacaaggaaa tcttcttcga tcagtctaag aacggatacg ctggatacat cgatggagga
     2461 gcttctcagg aggagttcta caagttcatc aagccaatcc ttgagaagat ggatggaacc
     2521 gaggagcttc ttgttaagtt gaacagagag gatcttctta gaaagcagag aaccttcgat
     2581 aacggatcta tcccacacca gatccacctt ggagagcttc acgctatcct tcgtagacag
     2641 gaggatttct acccattctt gaaggataac agagagaaga tcgagaagat ccttaccttc
     2701 agaatcccat actacgttgg accacttgct agaggaaact ctcgtttcgc ttggatgacc
     2761 agaaagtctg aggaaaccat caccccttgg aacttcgagg aggtaagttt ctgcttctac
     2821 ctttgatata tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt
     2881 tttcaaaata aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat
     2941 attttaattt ataacttttc taatatatga ccaaaatttg ttgatgtgca ggttgttgat
     3001 aagggagctt ctgctcagtc tttcatcgag agaatgacca acttcgataa gaaccttcca
     3061 aacgagaagg ttcttccaaa gcactctctt ctttacgagt acttcaccgt ttacaacgag
     3121 cttaccaagg ttaagtacgt taccgaggga atgagaaagc cagctttcct ttctggagag
     3181 cagaagaagg ctatcgttga tcttcttttc aagaccaaca gaaaggttac cgttaagcag
     3241 ttgaaggagg attacttcaa gaagatcgag tgcttcgatt ctgttgaaat ctctggagtt
     3301 gaggatagat tcaacgcttc tcttggaacc taccacgatc ttttgaagat catcaaggat
     3361 aaggatttcc ttgataacga ggagaacgag gacatccttg aggacatcgt tcttaccctt
     3421 acccttttcg aggatagaga gatgatcgag gagagactca agacctacgc tcaccttttc
     3481 gatgataagg ttatgaagca gttgaagaga agaagataca ccggatgggg tagactttct
     3541 cgtaagttga tcaacggaat cagagataag cagtctggaa agaccatcct tgatttcttg
     3601 aagtctgatg gattcgctaa cagaaacttc atgcagctta tccacgatga ttctcttacc
     3661 ttcaaggagg acatccagaa ggctcaggtt tctggacagg gagattctct tcacgagcac
     3721 atcgctaacc ttgctggatc tccagctatc aagaagggaa tccttcagac cgttaaggtt
     3781 gttgatgagc ttgttaaggt tatgggtaga cacaagccag agaacatcgt tatcgagatg
     3841 gctagagaga accagaccac ccagaaggga cagaagaact ctcgtgagag aatgaagaga
     3901 atcgaggagg gaatcaagga gcttggatct caaatcttga aggagcaccc agttgagaac
     3961 acccagcttc agaacgagaa gttgtacctt tactaccttc agaacggaag agatatgtac
     4021 gttgatcagg agcttgacat caacagactt tctgattacg atgttgatca catcgttcca
     4081 cagtctttct tgaaggatga ttctatcgat aacaaggttc ttacccgttc tgataagaac
     4141 agaggaaagt ctgataacgt tccatctgag gaggttgtta agaagatgaa gaactactgg
     4201 agacagcttc ttaacgctaa gttgatcacc cagagaaagt tcgataacct taccaaggct
     4261 gagagaggag gactttctga gcttgataag gctggattca tcaagagaca gcttgttgaa
     4321 accagacaga tcaccaagca cgttgctcag atccttgatt ctcgtatgaa caccaagtac
     4381 gatgagaacg ataagttgat cagagaggtt aaggttatca ccttgaagtc taagttggtt
     4441 tctgatttca gaaaggattt ccagttctac aaggttagag agatcaacaa ctaccaccac
     4501 gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc ttatcaagaa gtacccaaag
     4561 ttggagtctg agttcgttta cggagattac aaggtttacg atgttagaaa gatgatcgct
     4621 aagtctgagc aggagatcgg aaaggctacc gctaagtact tcttctactc taacatcatg
     4681 aacttcttca agaccgagat cacccttgct aacggagaga tcagaaagag gccacttatc
     4741 gaaaccaacg gagaaaccgg agagatcgtt tgggataagg gaagagattt cgctaccgtt
     4801 agaaaggttc tttctatgcc acaggttaac atcgttaaga aaaccgaggt tcagaccgga
     4861 ggattctcta aggagtctat ccttccaaag agaaactctg ataagttgat cgctagaaag
     4921 aaggattggg acccaaagaa gtacggagga ttcgattctc caaccgttgc ttactctgtt
     4981 cttgttgttg ctaaggttga gaagggaaag tctaagaagt tgaagtctgt taaggagctt
     5041 cttggaatca ccatcatgga gcgttcttct ttcgagaaga acccaatcga tttccttgag
     5101 gctaagggat acaaggaggt taagaaggat cttatcatca agttgccaaa gtactctctt
     5161 ttcgagcttg agaacggaag aaagagaatg cttgcttctg ctggagagct tcagaaggga
     5221 aacgagcttg ctcttccatc taagtacgtt aacttccttt accttgcttc tcactacgag
     5281 aagttgaagg gatctccaga ggataacgag cagaagcagc ttttcgttga gcagcacaag
     5341 cactaccttg atgagatcat cgagcaaatc tctgagttct ctaagagagt tatccttgct
     5401 gatgctaacc ttgataaggt tctttctgct tacaacaagc acagagataa gccaatcaga
     5461 gagcaggctg agaacatcat ccaccttttc acccttacca accttggtgc tccagctgct
     5521 ttcaagtact tcgataccac catcgataga aaaagataca cctctaccaa ggaggttctt
     5581 gatgctaccc ttatccacca gtctatcacc ggactttacg agaccagaat cgatctttct
     5641 cagcttggag gagataagag accagctgct accaagaagg ctggacaggc taagaagaag
     5701 aagtgaaaag ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc
     5761 agtttcattg cgcacacacc agaatcctac tgagtttgag tattatggca ttgggaaaac
     5821 tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc
     5881 tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt
     5941 tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat
     6001 tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat
     6061 gaccgaagtt aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag
     6121 gcaacaaata tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc
     6181 tcttgtgttt tagacattta tgaactttcc tttatgtaat tttccagaat ccttgtcaga
     6241 ttctaatcat tgctttataa ttatagttat actcatggat ttgtagttga gtatgaaaat
     6301 attttttaat gcattttatg acttgccaat tgattgacaa catgcatcaa tcggtaccac
     6361 ctagtagaag agctcagtga gaccgaattc gcttactaaa agccagataa cagtatgcgt
     6421 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     6481 caaaaagagg tgtgctaagc ttcggatgcc gggagcagac aagcccgtca gggcgcgtca
     6541 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     6601 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     6661 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6721 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6781 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtaca
     6841 cagggatctt caagagtcga cctgcaggca tgcaggcttg gcgtaatcat ggtcatagct
     6901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7141 gcgctcggtc gttcggctgc ggcgagcggt atcactagta ggaggtaatc aatgctggcc
     7201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7321 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     7381 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     7441 tgatcatctg gcggacctct tcggaggtcc gctttttttt ctcgagggtc tcaactagct
     7501 cttcatgggt attctagaga taatttattt gaaaattcat aagaaaagca aacgttacat
     7561 gaattgatga aacaatacaa agacagataa agccacgcac atttaggata ttggccgaga
     7621 ttactgaata ttgagtaaga tcacggaatt tctgacagga gcatgtcttc aattcagccc
     7681 aaatggcagt tgaaatactc aaaccgcccc atatgcagga gcggatcatt cattgtttgt
     7741 ttggttgcct ttgccaacat gggagtccaa gagctctcac ttgtacagct cgtccatgcc
     7801 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
     7861 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
     7921 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
     7981 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
     8041 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
     8101 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
     8161 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
     8221 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
     8281 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
     8341 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
     8401 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
     8461 gttatccatt gttcgtaccg ttttgctatt tgtgtatgtt ttcttgtttt ggtttgagtg
     8521 aagagtgtag tgaagggttg gtttgtattt ataggtgaga aaggagattt gcatggcaat
     8581 cacgtgtaag aatgcatgca tgcatgagtg tgtgtggaga gagagagatg ctcttacgtt
     8641 gttgtcggga ttatgtgttg gaaaagagtg acaagtgacg aacaaaacaa taccactcat
     8701 catagctccg cttatgcatg ctatgtgtga tttgtagctc ttgttctgac atgacactat
     8761 gaacaatttt ttaaaaagaa ggttaaactt ttatttaaat aaaaacccta tgttaatttg
     8821 gtttcaatcg agtaagatga gaagtcaatc ttttgatggt agctagtgat gttggaaata
     8881 tagttgtgcc agcttatcta gaccaagcat gcacagctgg cgttctcagc cagatcccct
     8941 tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt
     9001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9541 gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9781 tctaaagtat atatgtgtaa cattggtcta gtgattattt gccgactacc ttggtgatct
     9841 cgcctttcac gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
     9901 cttgtccaag ataagcctgc ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     9961 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
    10021 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
    10081 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
    10141 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
    10201 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
    10261 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
    10321 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
    10381 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
    10441 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
    10501 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
    10561 ctgatagttg agtcgatact tcggcgatca ccgcttccct cataacaccc cttgtattac
    10621 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
    10681 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
    10741 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
    10801 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
    10861 tctggctgga tgatggggcg attcaggcga tcc
//
