LOCUS       sequence-32547-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_EWSR1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      136..145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             145..168
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             169..195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    211..235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gagctcggat cgatccatcg atagtgtggt
      121 ggaattctga tcgccgccgc catggactac aaggacgacg atgacaagta cccttatgac
      181 gtgcccgatt acgctttgaa ccagatatca acaagtttgt acaaaaaagc aggctccacc
      241 atgggaacca attcagtcga catggcgtcc acggattaca gtacctatag ccaagctgca
      301 gcgcagcagg gctacagtgc ttacaccgcc cagcccactc aaggatatgc acagaccacc
      361 caggcatatg ggcaacaaag ctatggaacc tatggacagc ccactgatgt cagctatacc
      421 caggctcaga ccactgcaac ctatgggcag accgcctatg caacttctta tggacagcct
      481 cccactggtt atactactcc aactgccccc caggcataca gccagcctgt ccaggggtat
      541 ggcactggtg cttatgatac caccactgct acagtcacca ccacccaggc ctcctatgca
      601 gctcagtctg catatggcac tcagcctgct tatccagcct atgggcagca gccagcagcc
      661 actgcaccta caagaccgca ggatggaaac aagcccactg agactagtca acctcaatct
      721 agcacagggg gttacaacca gcccagccta ggatatggac agagtaacta cagttatccc
      781 caggtacctg ggagctaccc catgcagcca gtcactgcac ctccatccta ccctcctacc
      841 agctattcct ctacacagcc gactagttat gatcagagca gttactctca gcagaaca
//
