LOCUS       sequence-325471-        9342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Ren Q, Sretenovic S, Liu G, Zhong Z, Wang J, Huang L, Tang X, Guo Y,
            Liu L, Wu Y, Zhou J, Zhao Y, Yang H, He Y, Liu S, Yin D, Mayorga R, 
            Zheng X, Zhang T, Qi Y, Zhang Y
  TITLE     Improved plant cytosine base editors with high editing activity, 
            purity, and specificity.
  JOURNAL   Plant Biotechnol J. 2021 May 27. doi: 10.1111/pbi.13635.
  PUBMED    34042262
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2021 May 27. doi: 10.1111/pbi.13635."
COMMENT     Sequence Label: pYPQ269A2
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2576..3262
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             3317..3382
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3389..3409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3434..3457,3458..3460,3461..7534)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7447..7455
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7535..7582
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7604..7852
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7865..7885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7892..7945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7952..7972
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8000..8386
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8435..8683
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8696..8716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8723..8770
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8792..9044
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9089..9212
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9229..9248)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9230..9248)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9253..9269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9253..9269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgag
     2581 ttctgaaaca ggtccagtgg ctgtcgatcc gactctgagg aggcgcatcg aaccacacga
     2641 atttgaggtt ttttttgatc caagagagct tcgcaaggaa acttgtctcc tgtatgagat
     2701 aaattgggga ggaagacata gtatctggag acacacaagc cagaacacca ataagcatgt
     2761 cgaagttaac ttcatcgaaa aatttacgac cgaacgctac ttttgtccta atacacgctg
     2821 cagtatcact tggttccttt cgtggtcacc ttgcggtgaa tgttccagag caattacgga
     2881 gtttctctcg agatatccac acgttacact tttcatttac atagctcggc tttatcatca
     2941 cgcagatccg agaaatcgcc aggggctgcg cgatctgatt tcgtccggtg tcaccattca
     3001 aattatgact gagcaggaat ctggatactg ctggcgcaac ttcgtgaact acagtccgtc
     3061 gaatgaagcg cactggccga gatatcccca tctctgggtg cgcttgtatg ttttggagct
     3121 ttattgtatc atcttgggat tgccaccttg tcttaatata ttgcgccgga aacaaccgca
     3181 gctcactttt ttcaccattg ctctgcagtc ttgtcactac caacgcctcc ccccgcacat
     3241 cctgtgggcc acaggcctta aatcaggttc tgagacccct ggcacaagtg agtcagcaac
     3301 acccgagtcc accatggatt acaaggacca cgacggggat tacaaggacc acgacattga
     3361 ttacaaggat gatgatgaca agatggctcc gaagaagaag aggaaggttg gcatccacgg
     3421 ggtgccagct gctgacaaga agtactcgat cggcctcgct attgggacta actctgttgg
     3481 ctgggccgtg atcaccgacg agtacaaggt gccctcaaag aagttcaagg tcctgggcaa
     3541 caccgatcgg cattccatca agaagaatct cattggcgct ctcctgttcg acagcggcga
     3601 gacggctgag gctacgcggc tcaagcgcac cgcccgcagg cggtacacgc gcaggaagaa
     3661 tcgcatctgc tacctgcagg agattttctc caacgagatg gcgaaggttg acgattcttt
     3721 cttccacagg ctggaggagt cattcctcgt ggaggaggat aagaagcacg agcggcatcc
     3781 aatcttcggc aacattgtcg acgaggttgc ctaccacgag aagtacccta cgatctacca
     3841 tctgcggaag aagctcgtgg actccacaga taaggcggac ctccgcctga tctacctcgc
     3901 tctggcccac atgattaagt tcaggggcca tttcctgatc gagggggatc tcaacccgga
     3961 caatagcgat gttgacaagc tgttcatcca gctcgtgcag acgtacaacc agctcttcga
     4021 ggagaacccc attaatgcgt caggcgtcga cgcgaaggct atcctgtccg ctaggctctc
     4081 gaagtctcgg cgcctcgaga acctgatcgc ccagctgccg ggcgagaaga agaacggcct
     4141 gttcgggaat ctcattgcgc tcagcctggg gctcacgccc aacttcaagt cgaatttcga
     4201 tctcgctgag gacgccaagc tgcagctctc caaggacaca tacgacgatg acctggataa
     4261 cctcctggcc cagatcggcg atcagtacgc ggacctgttc ctcgctgcca agaatctgtc
     4321 ggacgccatc ctcctgtctg atattctcag ggtgaacacc gagattacga aggctccgct
     4381 ctcagcctcc atgatcaagc gctacgacga gcaccatcag gatctgaccc tcctgaaggc
     4441 gctggtcagg cagcagctcc ccgagaagta caaggagatc ttcttcgatc agtcgaagaa
     4501 cggctacgct gggtacattg acggcggggc ctctcaggag gagttctaca agttcatcaa
     4561 gccgattctg gagaagatgg acggcacgga ggagctgctg gtgaagctca atcgcgagga
     4621 cctcctgagg aagcagcgga cattcgataa cggcagcatc ccacaccaga ttcatctcgg
     4681 ggagctgcac gctatcctga ggaggcagga ggacttctac cctttcctca aggataaccg
     4741 cgagaagatc gagaagattc tgactttcag gatcccgtac tacgtcggcc cactcgctag
     4801 gggcaactcc cgcttcgctt ggatgacccg caagtcagag gagacgatca cgccgtggaa
     4861 cttcgaggag gtggtcgaca agggcgctag cgctcagtcg ttcatcgaga ggatgacgaa
     4921 tttcgacaag aacctgccaa atgagaaggt gctccctaag cactcgctcc tgtacgagta
     4981 cttcacagtc tacaacgagc tgactaaggt gaagtatgtg accgagggca tgaggaagcc
     5041 ggctttcctg tctggggagc agaagaaggc catcgtggac ctcctgttca agaccaaccg
     5101 gaaggtcacg gttaagcagc tcaaggagga ctacttcaag aagattgagt gcttcgattc
     5161 ggtcgagatc tctggcgttg aggaccgctt caacgcctcc ctggggacct accacgatct
     5221 cctgaagatc attaaggata aggacttcct ggacaacgag gagaatgagg atatcctcga
     5281 ggacattgtg ctgacactca ctctgttcga ggaccgggag atgatcgagg agcgcctgaa
     5341 gacttacgcc catctcttcg atgacaaggt catgaagcag ctcaagagga ggaggtacac
     5401 cggctggggg aggctgagca ggaagctcat caacggcatt cgggacaagc agtccgggaa
     5461 gacgatcctc gacttcctga agagcgatgg cttcgcgaac cgcaatttca tgcagctgat
     5521 tcacgatgac agcctcacat tcaaggagga tatccagaag gctcaggtga gcggccaggg
     5581 ggactcgctg cacgagcata tcgcgaacct cgctggctcg ccagctatca agaaggggat
     5641 tctgcagacc gtgaaggttg tggacgagct ggtgaaggtc atgggcaggc acaagcctga
     5701 gaacatcgtc attgagatgg cccgggagaa tcagaccacg cagaagggcc agaagaactc
     5761 acgcgagagg atgaagagga tcgaggaggg cattaaggag ctggggtccc agatcctcaa
     5821 ggagcacccg gtggagaaca cgcagctgca gaatgagaag ctctacctgt actacctcca
     5881 gaatggccgc gatatgtatg tggaccagga gctggatatt aacaggctca gcgattacga
     5941 cgtcgatcat atcgttccac agtcattcct gaaggatgac tccattgaca acaaggtcct
     6001 caccaggtcg gacaagaacc ggggcaagtc tgataatgtt ccttcagagg aggtcgttaa
     6061 gaagatgaag aactactggc gccagctcct gaatgccaag ctgatcacgc agcggaagtt
     6121 cgataacctc acaaaggctg agaggggcgg gctctctgag ctggacaagg cgggcttcat
     6181 caagaggcag ctggtcgaga cacggcagat cactaagcac gttgcgcaga ttctcgactc
     6241 acggatgaac actaagtacg atgagaatga caagctgatc cgcgaggtga aggtcatcac
     6301 cctgaagtca aagctcgtct ccgacttcag gaaggatttc cagttctaca aggttcggga
     6361 gatcaacaat taccaccatg cccatgacgc gtacctgaac gcggtggtcg gcacagctct
     6421 gatcaagaag tacccaaagc tcgagagcga gttcgtgtac ggggactaca aggtttacga
     6481 tgtgaggaag atgatcgcca agtcggagca ggagattggc aaggctaccg ccaagtactt
     6541 cttctactct aacattatga atttcttcaa gacagagatc actctggcca atggcgagat
     6601 ccggaagcgc cccctcatcg agacgaacgg cgagacgggg gagatcgtgt gggacaaggg
     6661 cagggatttc gcgaccgtca ggaaggttct ctccatgcca caagtgaata tcgtcaagaa
     6721 gacagaggtc cagactggcg ggttctctaa ggagtcaatt ctgcctaagc ggaacagcga
     6781 caagctcatc gcccgcaaga aggactggga tccgaagaag tacggcgggt tcgacagccc
     6841 cactgtggcc tactcggtcc tggttgtggc gaaggttgag aagggcaagt ccaagaagct
     6901 caagagcgtg aaggagctgc tggggatcac gattatggag cgctccagct tcgagaagaa
     6961 cccgatcgat ttcctggagg cgaagggcta caaggaggtg aagaaggacc tgatcattaa
     7021 gctccccaag tactcactct tcgagctgga gaacggcagg aagcggatgc tggcttccgc
     7081 tggcgagctg cagaagggga acgagctggc tctgccgtcc aagtatgtga acttcctcta
     7141 cctggcctcc cactacgaga agctcaaggg cagccccgag gacaacgagc agaagcagct
     7201 gttcgtcgag cagcacaagc attacctcga cgagatcatt gagcagattt ccgagttctc
     7261 caagcgcgtg atcctggccg acgcgaatct ggataaggtc ctctccgcgt acaacaagca
     7321 ccgcgacaag ccaatcaggg agcaggctga gaatatcatt catctcttca ccctgacgaa
     7381 cctcggcgcc cctgctgctt tcaagtactt cgacacaact atcgatcgca agaggtacac
     7441 aagcactaag gaggtcctgg acgcgaccct catccaccag tcgattaccg gcctctacga
     7501 gacgcgcatc gacctgtctc agctcggggg cgacaagcgg ccagcggcga cgaagaaggc
     7561 ggggcaggcg aagaagaaga agggagacgg atcaggcggg tcaacaaacc tcagtgacat
     7621 catagagaag gaaactggta agcaactggt tattcaagag agcattctga tgctccctga
     7681 ggaagtcgag gaagttatag gaaacaagcc tgagagcgat atactcgtgc atacggcgta
     7741 tgacgagagc acggatgaaa atgtgatgct cttgaccagc gatgcgccag aatataaacc
     7801 ctgggcactg gttattcagg actctaacgg ggaaaataaa atcaagatgc tctcaggtgg
     7861 ctccccaaag aagaaacgca aggtcaattg ggagggcaga ggaagtcttc taacatgcgg
     7921 tgacgtggag gagaatcccg gccctatggc tcctaagaag aagcggaagg ttggtattca
     7981 cggggtgcct gcggctatgg cgtcaaattt cacgcagttt gttttggttg ataacggcgg
     8041 gactggcgac gttacagtag ctccatcaaa ttttgcgaac ggagtcgctg agtggattag
     8101 ctcaaattca aggtcccagg cctacaaggt tacctgttct gttaggcaga gttctgcgca
     8161 aaaaagaaaa tataccatca aggttgaagt ccctaaagtt gcaacacaaa cagtcggtgg
     8221 tgttgagctc cctgtggcag cctggagatc ttacttaaac atggagctaa caattccaat
     8281 attcgctaca aactctgatt gtgaactgat tgttaaggcg atgcaaggtc tcttgaaaga
     8341 tggaaaccct ataccgtccg ctatcgcagc taacagcggt atctatggct ctggatcggg
     8401 gtcgggttct ggctcaacta gttcaggcgg gtcaacaaac ctcagtgaca tcatagagaa
     8461 ggaaactggt aagcaactgg ttattcaaga gagcattctg atgctccctg aggaagtcga
     8521 ggaagttata ggaaacaagc ctgagagcga tatactcgtg catacggcgt atgacgagag
     8581 cacggatgaa aatgtgatgc tcttgaccag cgatgcgcca gaatataaac cctgggcact
     8641 ggttattcag gactctaacg gggaaaataa aatcaagatg ctctcaggtg gctccccaaa
     8701 gaagaaacgc aaggttggta ccaagcgtcc tgctgccacc aaaaaggccg gacaggctaa
     8761 gaaaaagaag tagtggcggc cgccgacgtc cgatcgttca aacatttggc aataaagttt
     8821 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     8881 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     8941 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     9001 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tgatcccccc
     9061 tcgacagctt ccggaaaggg cgaattcgca actttgtata caaaagttga acgagaaacg
     9121 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     9181 tactgtaaaa cacaacatat ccagtcacta tgccatccag ctgatatccc ctatagtgag
     9241 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     9301 tgttacattg cacaagataa aaatatatca tcatgcctcc tc
//
