LOCUS       sequence-325477-        6327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Atoh1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Menendez L, Trecek T, Gopalakrishnan S, Tao L, Markowitz AL, Yu HV, 
            Wang X, Llamas J, Huang C, Lee J, Kalluri R, Ichida J, Segil N
  TITLE     Generation of inner ear hair cells by direct lineage conversion of 
            primary somatic cells.
  JOURNAL   Elife. 2020 Jun 30;9. pii: 55249. doi: 10.7554/eLife.55249.
  PUBMED    32602462
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-30"; volume: "9. pii: 55249. doi"; pages: " 
            10.7554/eLife.55249"
COMMENT     Sequence Label: pMXs-Atoh1
FEATURES             Location/Qualifiers
     source          1..6327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..358
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             417..833
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     763..785
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     801..817
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    843..1217
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1155..1171
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1260..1284
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2346..2370)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2387..2975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2440..2460)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2858..2869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3155..3624
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3754..3771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3925..4513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4005..4024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4677..5468,5469..5537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5300..5319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5538..5642)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5672..6265
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
       61 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
      121 tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg
      181 tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc
      241 cctaatagga gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
      301 cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca
      361 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
      421 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      481 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
      541 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
      601 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
      661 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
      721 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
      781 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
      841 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
      901 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
      961 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1021 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1081 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1141 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1201 gaccatcctc tagactgccg gatctagcta gttaatcgac ggtatcgata agctatcaaa
     1261 caagtttgta caaaaaagca ggctgccacc atgtcccgcc tgctgcatgc agaagagtgg
     1321 gctgaggtaa aagagttggg ggaccaccat cgccatcccc agccgcacca cgtcccgccg
     1381 ctgacgccac agccacctgc taccctgcag gcgagagacc ttcccgtcta cccggcagaa
     1441 ctgtccctcc tggatagcac cgacccacgc gcctggctga ctcccacttt gcagggcctc
     1501 tgcacggcac gcgccgccca gtatctgctg cattctcccg agctgggtgc ctccgaggcc
     1561 gcggcgcccc gggacgaggc tgacagccag ggtgagctgg taaggagaag cggctgtggc
     1621 ggcctcagca agagccccgg gcccgtcaaa gtacgggaac agctgtgcaa gctgaagggt
     1681 ggggttgtag tggacgagct tggctgcagc cgccagcgag ccccttccag caaacaggtg
     1741 aatggggtac agaagcaaag gaggctggca gcaaacgcaa gggaacggcg caggatgcac
     1801 gggctgaacc acgccttcga ccagctgcgc aacgttatcc cgtccttcaa caacgacaag
     1861 aagctgtcca aatatgagac cctacagatg gcccagatct acatcaacgc tctgtcggag
     1921 ttgctgcaga ctcccaatgt cggagagcaa ccgccgccgc ccacagcttc ctgcaaaaat
     1981 gaccaccatc accttcgcac cgcctcctcc tatgaaggag gtgcgggcgc ctctgcggta
     2041 gctggggctc agccagcccc gggagggggc ccgagaccta ccccgcccgg gccttgccgg
     2101 actcgcttct caggcccagc ttcctctggg ggttactcgg tgcagctgga cgctttgcac
     2161 ttcccagcct tcgaggacag ggccctaaca gcgatgatgg cacagaagga cctgtcgcct
     2221 tcgctgcccg ggggcatcct gcagcctgta caggaggaca acagcaaaac atctcccaga
     2281 tcccacagaa gtgacggaga gttttccccc cactctcatt acagtgactc tgatgaggcc
     2341 agttagacca gctttcttgt acaaagtggt tcgatagctt atcgataatc aacctctgga
     2401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2761 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     2821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2941 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac gataaaataa
     3001 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     3061 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
     3121 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     3181 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     3241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     3301 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     3361 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     3421 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     3481 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     3541 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     3601 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
     3661 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
     3721 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3781 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3841 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3901 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3961 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4021 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4081 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4141 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4201 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4261 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4321 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4381 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4441 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4501 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4561 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4621 tttgcggccg gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5641 cgcacatttc cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca
     5701 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa
     5761 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     5821 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     5881 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     5941 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     6001 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     6061 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
     6121 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     6181 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     6241 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     6301 gggaccaccg acccaccacc gggaggt
//
