LOCUS       sequence-325479-        6289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human Pou4f3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Menendez L, Trecek T, Gopalakrishnan S, Tao L, Markowitz AL, Yu HV, 
            Wang X, Llamas J, Huang C, Lee J, Kalluri R, Ichida J, Segil N
  TITLE     Generation of inner ear hair cells by direct lineage conversion of 
            primary somatic cells.
  JOURNAL   Elife. 2020 Jun 30;9. pii: 55249. doi: 10.7554/eLife.55249.
  PUBMED    32602462
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-30"; volume: "9. pii: 55249. doi"; pages: " 
            10.7554/eLife.55249"
COMMENT     Sequence Label: pMXs-Pou4f3
FEATURES             Location/Qualifiers
     source          1..6289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..358
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             417..833
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     763..785
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     801..817
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    843..1217
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1155..1171
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1260..1284
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2308..2332)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2349..2937
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2402..2422)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2820..2831)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3117..3586
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3716..3733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3887..4475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3967..3986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4639..5430,5431..5499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5262..5281
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5500..5604)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5634..6227
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
       61 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
      121 tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg
      181 tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc
      241 cctaatagga gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
      301 cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca
      361 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
      421 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      481 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
      541 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
      601 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
      661 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
      721 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
      781 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
      841 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
      901 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
      961 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1021 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1081 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1141 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1201 gaccatcctc tagactgccg gatctagcta gttaatcgac ggtatcgata agctatcaaa
     1261 caagtttgta caaaaaagca ggctgccacc atgatggcca tgaactccaa gcagcctttc
     1321 ggcatgcacc cggtgctgca agaacccaaa ttctccagtc tgcactctgg ctccgaggct
     1381 atgcgccgag tctgtctccc agccccgcag ctgcagggta atatatttgg aagctttgat
     1441 gagagcctgc tggcacgcgc cgaagctctg gcggcggtgg atatcgtctc ccacggcaag
     1501 aaccatccgt tcaagcccga cgccacctac cataccatga gcagcgtgcc ctgcacgtcc
     1561 acttcgtcca ccgtgcccat ctcccaccca gctgcgctca cctcacaccc tcaccacgcc
     1621 gtgcaccagg gcctcgaagg cgacctgctg gagcacatct cgcccacgct gagtgtgagc
     1681 ggcctgggcg ctccggaaca ctcggtgatg cccgcacaga tccatccaca ccacctgggc
     1741 gccatgggcc acctgcacca ggccatgggc atgagtcacc cgcacaccgt ggcccctcat
     1801 agcgccatgc ctgcatgcct cagcgacgtg gagtcagacc cgcgcgagct ggaagccttc
     1861 gccgagcgct tcaagcagcg gcgcatcaag ctgggggtga cccaggcgga cgtgggcgcg
     1921 gctctggcta atctcaagat ccccggcgtg ggctcgctga gccaaagcac catctgcagg
     1981 ttcgagtctc tcactctctc gcacaacaac atgatcgctc tcaagccggt gctccaggcc
     2041 tggttggagg aggccgaggc cgcctaccga gagaagaaca gcaagccaga gctcttcaac
     2101 ggcagcgaac ggaagcgcaa acgcacgtcc atcgcggcgc cggagaagcg ttcactcgag
     2161 gcctatttcg ctatccagcc acgtccttca tctgagaaga tcgcggccat cgctgagaaa
     2221 ctggacctta aaaagaacgt ggtgagagtc tggttctgca accagagaca gaaacagaaa
     2281 cgaatgaagt attcggctgt ccactgaacc cagctttctt gtacaaagtg gttcgatagc
     2341 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2401 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2461 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2521 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2581 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2641 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2701 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2761 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2821 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2881 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2941 gataccgtcg acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     3001 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     3061 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
     3121 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     3181 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3241 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     3301 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     3361 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     3421 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     3481 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     3541 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
     3601 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
     3661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3841 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3901 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3961 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4021 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4081 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4141 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4201 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4261 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4321 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4381 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4441 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4501 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4561 aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga
     4621 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4681 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4741 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4801 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4861 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4921 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4981 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5041 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5101 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5161 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5221 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5281 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5341 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5401 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5461 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5521 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5581 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cataatgaaa
     5641 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     5701 aatacataac tgagaataga aaagttcaga tcaaggtcag gaacagatgg aacagctgaa
     5761 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     5821 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     5881 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     5941 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     6001 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     6061 agcccacaac ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc
     6121 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     6181 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     6241 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggt
//
