LOCUS       sequence-32548-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_TO_TAF15
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             183..206
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             207..233
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    249..273
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacca
      181 tggactacaa ggacgacgat gacaagtacc cttatgacgt gcccgattac gctttgaacc
      241 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccaat tcagtcgaca
      301 tgtcggattc tggaagttac ggtcagtctg ggggtgagca gcaaagttat tctacctatg
      361 gaaatccagg cagccaaggc tatggacaag catcacaaag ctattctggc tatgggcaaa
      421 cgactgattc ctcttatgga cagaactaca gcggttactc cagttatgga caaagtcagt
      481 caggttattc acagtcctat ggtggttatg agaatcaaaa gcagagctca tatagccagc
      541 aaccatataa taaccaggga cagcagcaaa acatggaatc atcaggaagc caaggtggaa
      601 gagcaccttc ctatgaccag ccagactatg gtcaacaaga ttcatatgac cagcagtcag
      661 gctatgatca acatcaaggc tcatatgatg agcagtcaaa ttatgatcag cagcatgatt
      721 cctatagtca aaaccagcag tcctatcatt cacaaaggga aaactacagc caccacacac
      781 aagatgaccg tcgtgatgtg agtaggtatg gagaagataa tagaggatat ggcgggtcac
      841 agggaggagg tagagggcgt gggggatatg acaaggatgg aagaggtcct atgacag
//
