LOCUS       sequence-32549-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_TAF15
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             170..196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    212..236
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgagctcgga tcgatccatc gatagtgtgg
      121 tggaattctg atcgccgccg ccatggacta caaggacgac gatgacaagt acccttatga
      181 cgtgcccgat tacgctttga accagatatc aacaagtttg tacaaaaaag caggctccac
      241 catgggaacc aattcagtcg acatgtcgga ttctggaagt tacggtcagt ctgggggtga
      301 gcagcaaagt tattctacct atggaaatcc aggcagccaa ggctatggac aagcatcaca
      361 aagctattct ggctatgggc aaacgactga ttcctcttat ggacagaact acagcggtta
      421 ctccagttat ggacaaagtc agtcaggtta ttcacagtcc tatggtggtt atgagaatca
      481 aaagcagagc tcatatagcc agcaaccata taataaccag ggacagcagc aaaacatgga
      541 atcatcagga agccaaggtg gaagagcacc ttcctatgac cagccagact atggtcaaca
      601 agattcatat gaccagcagt caggctatga tcaacatcaa ggctcatatg atgagcagtc
      661 aaattatgat cagcagcatg attcctatag tcaaaaccag cagtcctatc attcacaaag
      721 ggaaaactac agccaccaca cacaagatga ccgtcgtgat gtgagtaggt atggagaaga
      781 taatagagga tatggcgggt cacagggagg aggtagaggg cgtgggggat atgacaagga
      841 tggaagaggt cctatgacag gatcaagtgg tggtgaccgc ggtggcttc
//
