LOCUS       sequence-325497-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter 
            with TAG mutation at site 35, for unnatural amino acid incorporation
            into the GFP protein in Vibrio natriegens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ozer E, Alfonta L
  TITLE     Genetic Code Expansion of Vibrio natriegens.
  JOURNAL   Front Bioeng Biotechnol. 2021 Feb 26;9:594429. doi: 
            10.3389/fbioe.2021.594429. eCollection 2021.
  PUBMED    33718334
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2021-02-26"; volume: "9:594429. doi"; 
            pages: " 10.3389/fbioe.2021.594429. eCollection 2021"
COMMENT     Sequence Label: pRaGE Pyl TAG GFP Y35TAG
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttactgcnng gtaggtcagg tttgttatca atgaagttaa tttcgttgaa ccccgcatcg
       61 agaggttatt tgggtatatg aacacggaag gattgtagta ttgacttacg atggtaaaat
      121 gtcgccactt ttatccttga caatacaacg agaaccctct cgttgaacat taaccctaag
      181 gaactcaaat ggataaaaaa ccactaaaca ctctgatctc tgctactggt ctgtggatga
      241 gtcgtaccgg aaccattcat aaaatcaaac accacgaggt tagccgttcg aaaatctata
      301 ttgagatggc gtgtggcgat catctggttg tgaacaatag ccgctcttct cgtacagcac
      361 gtgcactgcg tcaccacaaa tatcgtaaaa cctgtaaacg ttgccgtgtg ctgatgaatc
      421 ccctaatgat tttggtaaaa atcattaagt taaggtggat acacatcttg tcatatgatc
      481 aaatggtttc gcgaaaaatc aataatcaga caacaagatg tgcgaactcg atattttaca
      541 cgactctctt taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt
      601 ggcttgccac gcattacttg actgtaaaac tctcactctt accgaacttg gccgtaacct
      661 gccaaccaaa gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg
      721 tcacctccac aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg
      781 caatacgatg cccattgtac ttgttgactg gtctgatatt cgtgagcaaa aacgacttat
      841 ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt
      901 cccgctttca nagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag
      961 cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg
     1021 gnataaatcc gttgagaa
//
