LOCUS       sequence-325498-        9766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Smo-FLAG K434, 448R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: GP825
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..62)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        130..234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(235..303,304..1095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(453..472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1266..1854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2008..2025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2265..2286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2301..2306,2307..2324,2325..2331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2339..2355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2344..2366
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2363..2379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2363..2379
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2640..3273
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3320..3445
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3942..4175
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4360..4404
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4700..4817
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    4866..4914
                     /note="synthetic polyadenylation signal"
     misc_feature    4928..5019
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4968..4987
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    5034..5168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5071..5090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             5364..5759
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(5760..6333,6334..6336,6337..6345)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5926..5943)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6153..6172
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      8732..8741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8741..8806
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8847..9435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8900..8920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9318..9329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9583..9763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gggcgattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
       61 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      121 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      181 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      241 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      301 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      361 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      421 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
      481 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      541 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      601 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      661 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      721 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      781 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      841 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      901 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      961 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1021 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1081 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1141 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1201 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1321 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1381 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1441 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1501 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1561 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1621 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1681 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1741 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1801 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1861 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1921 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1981 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2041 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
     2101 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2161 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
     2221 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2281 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2341 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
     2401 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2461 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     2521 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     2581 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     2641 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     2701 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     2761 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     2821 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     2881 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     2941 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3001 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3061 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3121 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3181 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3241 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3301 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3361 aagaggcgag gggcggcgac tgcagagtac gccaaaaatt ttgactagcg gaggctagaa
     3421 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     3481 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     3541 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     3601 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     3661 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     3721 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     3781 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     3841 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     3901 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     3961 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4021 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4081 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4141 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4201 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4261 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4321 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4381 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     4441 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     4501 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     4561 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     4621 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tctcaacaat
     4681 aggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     4741 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     4801 caaaaattca aaattttcgg gtttattact cggacagcag agatccagtt tggactagtg
     4861 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     4921 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     4981 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgataggta ccgaacttgt
     5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5161 tctggatccc cgggaacaga ttcgcgacta gtggacacac aggatagggg tgggcagcat
     5221 gaggggggcc agagggagag ggggcagagc tgggctggac gaggggacac cccgccccaa
     5281 tggggcgtga cgagctgcca gcccaggctc cggggcacgt aaaagcgggg ctgtgagacc
     5341 ggagagcacg gagcgacatt aatatggcca agcctttgtc tcaagaagaa tccaccctca
     5401 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     5461 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     5521 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     5581 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     5641 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     5701 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc
     5761 ccctctccct ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5821 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5881 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     5941 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     6001 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     6061 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     6121 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     6181 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
     6241 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     6301 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccataga ccatggccgc
     6361 tggccgcccc gtgcgtgggc ccgagctggc gccccggagg ctgctgcagt tgctgctgct
     6421 ggtactgctg gggggcccgg gccggggggc ggccttgagc gggaacgtga ccgggcctgg
     6481 gcctcacagc gccagcggga gctcgaggag ggacgtgccg gtgaccagcc ctccgccgcc
     6541 gctgctgagc cactgcggcc gggccgccca ctgcgagcct ttgcgctaca acgtgtgcct
     6601 gggctcggcg ctgccctacg gagccaccac cacgctgctg gctggggact cggactcgca
     6661 ggaggaagcg cacggcaagc tcgtgctctg gtccggcctc cggaatgccc cccgctgctg
     6721 ggcagtgatc cagcccctgc tgtgtgctgt ctacatgccc aagtgtgaga atgaccgagt
     6781 ggagttgccc agccgtaccc tctgccaggc cacccgaggc ccctgtgcca ttgtggagcg
     6841 ggagcgaggg tggcctgact ttctgcgttg cacaccggac cacttccctg aaggctgccc
     6901 aaacgaggta caaaacatca agttcaacag ctcaggccaa tgtgaagcac ccttggtgcg
     6961 aacagacaac cccaagagct ggtatgagga cgtggagggc tgtgggattc agtgtcagaa
     7021 cccgctgttc accgaggccg agcaccagga catgcacagc tacatcgcag ccttcggggc
     7081 ggtcactggt ctctgcacgc tcttcacact ggccaccttt gtggctgact ggcggaactc
     7141 caatcgctac cctgcggtta ttctcttcta tgtcaatgcg tgtttcttcg tgggcagcat
     7201 tggctggctg gcccagttca tggatggtgc ccgccgagag attgtttgcc gagcagatgg
     7261 caccatgaga tttggggagc ccacctccag tgagaccctg tcctgtgtca tcatctttgt
     7321 cattgtgtac tatgccttga tggctggagt agtctggttc gtggtcctca cctatgcctg
     7381 gcacacctcc ttcaaagccc tgggcaccac ctaccagcct ctctcgggca agacatccta
     7441 tttccacctg ctcacgtggt cactcccctt tgtcctcacg gtggcaatcc tggctgtggc
     7501 tcaggtagat ggagactccg tgagtggcat ctgttttgta ggctacaaga actatcggta
     7561 ccgtgctggc tttgtcctgg ccccaattgg cctggtgctt attgtgggag gctacttcct
     7621 catcagaggg gtcatgactc tgttctccat caggagcaac caccctgggc ttctgagtga
     7681 gaaggcagcc agcaggatca acgagaccat gctgcgcctg ggcatttttg gcttcctggc
     7741 ctttggcttt gtgctcatca ccttcagctg ccacttctat gacttcttca accaggctga
     7801 gtgggagcgt agcttccggg actatgtgct atgccaagcc aacgtgacca tcgggctgcc
     7861 taccaagaag cccattcctg actgtgagat caagaatcgg cccagcctcc tggtggagaa
     7921 gatcaatcta tttgccatgt ttggcactgg cattgccatg agcacctggg tctggaccaa
     7981 ggccaccctg ctcatctgga ggcgcacctg gtgcaggttg actgggcaca gtgatgatga
     8041 gcccaagaga atcaagaaga gcaagatgat cgccaaggcc ttctctaagc ggcgtgagct
     8101 gctgcagaac ccgggccagg agctctcctt cagcatgcac actgtctccc atgatggacc
     8161 tgttgcgggt ttggcttttg acctcaatga accctcagct gacgtctcct ctgcctgggc
     8221 tcagcatgtc accaagatgg tggctcggag aggagccata ttgccccagg atgtgtccgt
     8281 tacccctgtg gcaactccag tgccaccaga agaacaagcc aacatgtggc tggttgaggc
     8341 agagatctcc ccagagttag agaagcgttt gggccggaag aaaaagcgga ggaagaggaa
     8401 gaaggaggtg tgccccttga ggccagcccc tgagcttcac cactctgccc ctgttcctgc
     8461 caccagtgca gttcctcggc tgcctcagct gcctcggcag aagtgcctgg tagctgcaaa
     8521 cgcctgggga acaggggagt cctgccgaca gggagcctgg actctagtct ccaacccctt
     8581 ctgcccagag cctagtcccc atcaagatcc atttctccct ggtgcctcag ccccccgggt
     8641 ctgggctcag ggccgcctcc aggggctggg atccattcat tcccgcacta acctaatgga
     8701 ggctgagatc ttggatgcag actcggactt cgccaccatg gactacaaag accatgacgg
     8761 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaaggcgg ccgcttagta
     8821 agtaagatcc gaactcgaga gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     8881 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8941 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     9001 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9061 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9121 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9181 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9241 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9301 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9361 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9421 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9481 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     9541 gatcacctgc aggacaggcg cgccctgctt tttgcttgta ctgggtctct ctggttagac
     9601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcaccc
//
