LOCUS       sequence-325505-       11209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of HSV-1 TK and firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Argaw T, Marino MP, Timmons A, Eldridge L, Takeda K, Li P, Kwilas A,
            Ou W, Reiser J
  TITLE     In vivo targeting of lentiviral vectors pseudotyped with the Tupaia 
            paramyxovirus H glycoprotein bearing a cell-specific ligand.
  JOURNAL   Mol Ther Methods Clin Dev. 2021 Apr 24;21:670-680. doi: 
            10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11.
  PUBMED    34141822
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2021-04-24"; volume: "21:670-680. doi"; 
            pages: " 10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11"
COMMENT     Sequence Label: pNL(CMV)TK2ALuc2/CMV/WPREdU3
FEATURES             Location/Qualifiers
     source          1..11209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(810..827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(981..1569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1061..1080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1740..2531,2532..2600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2363..2382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2601..2705)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2773..2791
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2829..2851)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2951..2970
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             4123..4303
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      4930..5065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    5343..5576
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5761..5805
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6075..6378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6379..6582
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6612..6621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7746..7811
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             7824..9476
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    9483..10071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9536..9556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9954..9965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10146..10379
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 tatcaatccg ggggtgccag ctgatccatg gagtgcaggg tctgcaaaat atctcaagca
       61 ctgattgatc ttaggtttta caatagtgat gttaccccag gaacaatttg gggaaggtca
      121 gaatcttgta gcctgtagct gcatgactcc taaaccataa tttctttttt gttttttttt
      181 ttttattttt gagacagggt ctcactctgt cacctaggct ggagtgcagt ggtgcaatca
      241 cagctcactg cagcctcaac gtcgtaagct caagcgatcc tcccacctca gcctgcctgg
      301 tagctgagac tacaagcgac gccccagtta atttttgtat ttttggtaga ggcagcgttt
      361 tgccgtgtgg ccctggctgg tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc
      421 caaagtgctg ggacaaccgg ggccagtcac tgcacctggc cctaaaccat aatttctaat
      481 cttttggcta atttgttagt cctacaaagg cagtctagtc cccaggcaaa aagggggttt
      541 gtttcgggaa agggctgtta ctgtctttgt ttcaaactat aaactaagtt cctcctaaac
      601 ttagttcggc ctacacccag gaatgaacaa ggagagcttg gaggttagaa gcacgatgga
      661 attggttagg tcagatctct ttcactgtct gagttataat tttgcaatgg tggttcaaag
      721 actgcccgct tctgacacca gtcgctgcat taatgaatcg gccaacgcgc ggggagaggc
      781 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      841 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      961 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1021 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1081 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1141 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1201 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1261 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1321 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1381 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1441 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1501 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1561 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1621 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1681 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1741 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1801 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1861 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1921 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1981 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2041 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2101 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2161 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2221 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2281 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2341 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2401 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2461 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2521 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2581 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2641 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2701 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     2761 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     2821 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     2881 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3001 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     3061 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccaggg gaggcagaga
     3121 ttgcagtaag ctgagatcgc agcactgcac tccagcctgg gcgacagagt aagactctgt
     3181 ctcaaaaata aaataaataa atcaatcaga tattccaatc ttttccttta tttatttatt
     3241 tattttctat tttggaaaca cagtccttcc ttattccaga attacacata tattctattt
     3301 ttctttatat gctccagttt tttttagacc ttcacctgaa atgtgtgtat cgagacctag
     3361 aaaaacatgg agcaatcaca agtagcaata cagcagctaa caatgctgct tgtgcctggc
     3421 tagaagcaca agaggaggaa gaggtgggtt ttccagtcac acctcaggta cctttaagac
     3481 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3541 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     3601 aaggctactt ccctgattgg cagaactaca caccagggcc aggggtcaga tatccactga
     3661 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     3721 aaggagagaa caccagcttg ttacaccctg tgagcctgca tggaatggat gaccctgaga
     3781 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     3841 tgcatccgga gtatctagat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3901 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3961 acgccaatag ggactttcca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4021 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
     4081 actctagatg ctacatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     4141 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4201 agcttgcctt gagtgctcaa agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4261 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     4321 acttgaaagc gaaagtaaag ccagaggaga tctctcgacg caggactcgg cttgctgaag
     4381 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     4441 gaggctagaa ggagagagat gggtgcgaga gcgtcggtat taagcggggg agaattagat
     4501 aaatgggaaa aaattcggtt aaggccaggg ggaaagaaac aatataaact aaaacatata
     4561 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctttt agagacatca
     4621 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4681 cttagatcat tatataatac aatagcagtc ctctattgtg tgcatcaaag gatagatgta
     4741 aaagacacca aggaagcctt agataagata gaggaagagc aaaacaaaag taagaaaaag
     4801 gcacagcaag cagcagctga cacaggaaac aacagccagg tcagccaaaa ttaccctata
     4861 gtgcagaacc tccaggggca aatggtacat caggccatat cacctagaac tttaaatatg
     4921 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4981 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5041 tagtgaggag gcttttttgg aggcctaggc ttttgcaaat gcataaaaga aaagggggga
     5101 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5161 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5221 gagatccaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     5281 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     5341 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     5401 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     5461 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     5521 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     5581 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     5641 agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg ggacagagaa
     5701 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     5761 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     5821 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     5881 ttaagaatag tttttgctgt actttctgta gtgaatagag ttaggcaggg atattcacca
     5941 ttatcgtttc agacccacct cccaatcccg aggggacccg acaggcccga aggaatagaa
     6001 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctagttaa
     6061 ctagatctag agtccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6361 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     6421 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     6481 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     6541 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     6601 atccgctagc cgccaccatg gccagctacc cctgtcacca gcacgccagc gccttcgatc
     6661 aggccgccag aagcagaggc cacaacaaca gacggaccgc cctgcggccc agacggcagc
     6721 agaaagccac cgaagtgcgg ctggaacaga agatgcccac cctgctgcgg gtgtacatcg
     6781 acggccctca cggcatgggc aagaccacca ccacccagct gctggtggcc ctgggcagca
     6841 gggacgacat cgtgtacgtg cccgagccca tgacctactg gcgggtgctg ggcgccagcg
     6901 agacaatcgc caacatctac accacacagc accggctgga ccagggcgag atctctgctg
     6961 gcgacgccgc cgtggtgatg accagcgccc agatcaccat gggaatgccc tacgccgtga
     7021 ccgatgccgt gctggcccct cacattggcg gcgaggccgg atctagccac gcccctcccc
     7081 ctgccctgac catcttcctg gaccggcacc ctatcgccgc cctgctgtgt taccctgccg
     7141 ccagatacct gatgggcagc atgacccccc aggctgtgct ggccttcgtg gccctgatcc
     7201 cccctaccct gcctggcacc aacatcgtgc tgggagccct gcccgaggac cggcacatcg
     7261 acagactggc caagcggcag cggcctggcg agagactgga cctggccatg ctggccgcca
     7321 tcagacgggt gtacggcctg ctggctaaca ccgtgcgcta cctccagggc ggaggctctt
     7381 ggagagagga ctggggccag ctgagcggag ctgccgtgcc accacagggc gccgagcctc
     7441 agtctaacgc cggacccaga ccccacatcg gcgacaccct gtttaccctg ttcagagccc
     7501 ccgagctgct ggcccccaac ggcgacctgt acaacgtgtt cgcctgggct ctggacgtgc
     7561 tcgccaagag actgcggccc atgcacgtgt tcatcctgga ctacgaccag agccctgccg
     7621 gctgtaggga cgctctgctc cagctgacca gcggcatggt gcagacccac gtgaccaccc
     7681 ccggcagcat ccccaccatc tgcgacctgg ccagaacctt cgccagagag atgggcgagg
     7741 ccaacgtgaa gcagaccctg aacttcgacc tgctgaagct ggccggcgac gtggaaagca
     7801 accctggccc cggcggaggc agcatggaag atgccaaaaa cattaagaag ggcccagcgc
     7861 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
     7921 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
     7981 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
     8041 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
     8101 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
     8161 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
     8221 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
     8281 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
     8341 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
     8401 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
     8461 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
     8521 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
     8581 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
     8641 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
     8701 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
     8761 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
     8821 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
     8881 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
     8941 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
     9001 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
     9061 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
     9121 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
     9181 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
     9241 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
     9301 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
     9361 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
     9421 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaactcg
     9481 agaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9841 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     9901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgggtacc
    10081 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10141 gggactggaa gggctaattc actcccaaag aagacaagat ctgctttttg cctgtactgg
    10201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac
    10381 ccaggaggta gaggttgcag tgagccaaga tcgcgccact gcattccagc ctgggcaaga
    10441 aaacaagact gtctaaaata ataataataa gttaagggta ttaaatatat ttatacatgg
    10501 aggtcataaa aatatatata tttgggctgg gcgcagtggc tcacacctgc gcccggccct
    10561 ttgggaggcc gaggcaggtg gatcacctga gtttgggagt tccagaccag cctgaccaac
    10621 atggagaaac cccttctctg tgtattttta gtagatttta ttttatgtgt attttattca
    10681 caggtatttc tggaaaactg aaactgtttt tcctctactc tgataccaca agaatcatca
    10741 gcacagagga agacttctgt gatcaaatgt ggtgggagag ggaggttttc accagcacat
    10801 gagcagtcag ttctgccgca gactcggcgg gtgtccttcg gttcagttcc aacaccgcct
    10861 gcctggagag aggtcagacc acagggtgag ggctcagtcc ccaagacata aacacccaag
    10921 acataaacac ccaacaggtc caccccgcct gctgcccagg cagagccgat tcaccaagac
    10981 gggaattagg atagagaaag agtaagtcac acagagccgg ctgtgcggga gaacggagtt
    11041 ctattatgac tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact
    11101 tagtgtgtag ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt
    11161 gcgccgagtc agttcctggg tgggggccac aagatcggat gagccagtt
//
