LOCUS       sequence-325508-        6844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Argaw T, Marino MP, Timmons A, Eldridge L, Takeda K, Li P, Kwilas A,
            Ou W, Reiser J
  TITLE     In vivo targeting of lentiviral vectors pseudotyped with the Tupaia 
            paramyxovirus H glycoprotein bearing a cell-specific ligand.
  JOURNAL   Mol Ther Methods Clin Dev. 2021 Apr 24;21:670-680. doi: 
            10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11.
  PUBMED    34141822
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2021-04-24"; volume: "21:670-680. doi"; 
            pages: " 10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11"
COMMENT     Sequence Label: pCG-TPMV Hd32-IL-13
FEATURES             Location/Qualifiers
     source          1..6844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          325..898
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     906..925
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        953..971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      991..1000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3295..3312
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3505..3524)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3570..3625
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3624..3643)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3979..4114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4220..4237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4391..4979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4471..4490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5150..5941,5942..6010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5773..5792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6011..6115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6183..6201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6239..6261)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6361..6380
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6499..6517
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6541..6844
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg ggggatcgat
      301 ccccccgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
      361 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
      421 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
      481 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
      541 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
      601 aagtactttc tctaatcact ttttttttca aggcaatcag ggtatattat attgtacttc
      661 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
      721 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat
      781 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
      841 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
      901 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgtaatacga
      961 ctcactatag ggcgaattcg gatccctgcc gccaccatgg gacctccctc aactctgcag
     1021 cactactcaa atccccctcc atatagcgag gaagaccagg gaatcgatgg accacagcgg
     1081 agccagccac tgtctacccc tcaccagtac gatcgctact atggcgtgaa cattcagcac
     1141 acacgagtct ataatcatct ggggactatc tactccggac tgaagctggc tttccagatt
     1201 ctgggctggg tgtccgtcat cattacaatg atcattactg tgaccacact gaagaaaatg
     1261 agtgatggga acagccagga ctccgcaatg ctgaagtcac tggacgagaa tttcgatgct
     1321 atccaggaag tggcaaacct gctggacaat gaggtgcgcc ctaaactggg cgtcactatg
     1381 acccagacta cctttcagct gccaaaggag ctgtccgaga tcaagagata cctgctgcga
     1441 ctggagcgaa actgccccgt gtgcggaaca gaagcaactc cacagggatc taagggcaat
     1501 gccagtggag ataccgcttt ctgcccccct tgtctgacac gccagtgctc tgaggacagt
     1561 actcatgatc agggacccgg cgtggaagga acctctcgaa accacaaggg caaaatcaat
     1621 tttcctcata ttctgcagtc cgacgattgt gggcggtctg acaacctgat cgtgtatagt
     1681 attaatctgg tccctggact gagtttcatc cagctgccat caggcacaaa gcactgcatc
     1741 attgatgtga gttatacctt ttcagacaca ctggcaggct acctgattgt gggcggggtc
     1801 gatggatgtc agctgcataa caaagccatc atctacctgt ccctgggcta ctataagacc
     1861 aaaatgatct atccacccga ctacatcgca attgccacat acacttatga tctggtgcct
     1921 aacctgagag actgcagcat cgccgtcaat cagacatccc tggccgctat ttgtacttct
     1981 aagaaaacca aggagaacca ggatttctcc acttctggag tgcacccatt ctatatcttt
     2041 accctgaata cagatggcat ctttaccgtg acagtcattg aacagtctca gctgaagctg
     2101 gactaccagt atgcagccct gtacccagct accggaccag gaatcttcat tggcgaccat
     2161 ctggtgtttc tgatgtgggg aggcctgatg accaaagctg agggcgatgc atattgccag
     2221 gcaagcggat gtaacgacgc acaccgaaca tcatgcaata tcgcacagat gccaagcgcc
     2281 tacgggcatc ggcagctggt gaacggactg ctgatgctgc ccatcaagga gctgggaagc
     2341 cacctgattc agccctcact ggaaacaatc agccctaaga tcaactgggc cgggggacat
     2401 ggcaggctgt actataactg ggagatcaat acaacttaca tctatatcga aggaaagacc
     2461 tggaggtccc gccccaacct gggcatcatt agttggtcaa aacctctgag tatccggtgg
     2521 attgaccact cagtggctcg gagacctggc gcaagaccat gtgactctgc caatgattgc
     2581 cctgaggact gtctggtggg cgggtactat gatatgttcc caatgagctc cgactacaag
     2641 actgcaatca ccatcattcc cactcaccat cagtggcctt ctagtccagc cctgaaactg
     2701 tttaacacca atagagaagt gagggtggtc atgatcctga ggcctccaaa caatgtcaag
     2761 aaaaccacaa tttcctgcat ccgcattatg cagacaaact ggtgtctggg cttcatcatc
     2821 ttcaaggagg gcaacaatgc ttgggggcag atctactcct atatctacca ggtggaatcc
     2881 acttgcccaa atactaaaac ggccggaaga gctgcacagc cagctatgtc tccaggacca
     2941 gtgccaccta gtaccgccct gagggagctg atcgaggaac tggtcaacat tacacagaat
     3001 cagaaggctc ccctgtgcaa cggcagcatg gtgtggtcca tcaatctgac tgcagggatg
     3061 tactgcgccg ctctggagag cctgatcaac gtctcaggct gtagcgccat tgaaaagacc
     3121 cagagaatgc tgtccggctt ctgtcctcac aaagtgtctg ccgggcagtt ttcaagcctg
     3181 catgtcaggg ataccaagat cgaggtggct cagttcgtca aagacctgct gctgcacctg
     3241 aagaaactgt ttcgggaggg acagttcaac gcggccgcac ggggtaccgg gagccatcac
     3301 catcatcatc actgacgact agtgtgaaat agacatcaga attaagaaaa acgtagggtc
     3361 caagtggttc cccgttatgg actcgctatc tgtcaaccag atcttatacc ctgaagttca
     3421 cctagatagc ccgatagtta ccaataagat agtagccatc ctggagtatg ctcgactgtt
     3481 taaacctgca ggcatgcaag ctgatctttt tccctctgcc aaaaattatg gggacatcat
     3541 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     3601 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     3661 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     3721 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3781 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3841 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3901 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgagga
     3961 gctttttgca aaagcctagg cctccaaaaa agcctcttca ctacttctgg aatagctcag
     4021 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     4081 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     4141 ttgctgacta attgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4201 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4261 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4321 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4381 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4441 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4501 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4561 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4621 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4741 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4801 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4861 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4921 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5041 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5101 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5161 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5221 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5281 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5341 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5401 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5461 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5521 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5581 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5641 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5701 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5761 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5821 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5881 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5941 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6001 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6061 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6121 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     6181 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     6241 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6301 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6361 cggcatcaga gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc
     6421 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     6481 ggtctggctt atcgaaatta atacgactca ctatagggag accggaattc gagctcgccc
     6541 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     6601 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     6661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     6721 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     6781 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     6841 catg
//
