LOCUS       sequence-325512-       13570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtCEP12:T35s
FEATURES             Location/Qualifiers
     source          1..13570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      222..474
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    585..609
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      701..1332
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1419..1523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1524..1592,1593..2384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1742..1761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2555..3143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3479..4627
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4783..4807
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4929..5108
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5130..5924
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      5937..6189
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6284..6308
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6572..7201
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7630..8703
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8769..8963
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9246..9350
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9351..10145
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10316..10904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    12853..13027
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        13127..13306
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
       61 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
      121 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
      181 tctatcgcct tcttgacgag ttcttctgag cttgtcaagc agatcgttca aacatttggc
      241 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
      301 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
      361 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
      421 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct
      481 ggatccgaat tcggcattgt cttcacagag tggggcccac tgcatccacc ccagtacatt
      541 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
      601 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
      661 gatacaggca gcccatcagt cagatcagga tctcctttgc gacgctcacc gggctggttg
      721 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
      781 agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc
      841 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
      901 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
      961 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
     1021 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
     1081 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
     1141 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
     1201 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
     1261 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
     1321 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
     1381 ggcctcaccc caaaaatggc agcgctggcc aattcgtgcg cggaacccct atttgtttat
     1441 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccttga taaatgcttc
     1501 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1561 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1621 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1681 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1741 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1801 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1861 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1921 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1981 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2041 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2101 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2161 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2221 ctggagccgg tgagcgtggt tctcgcggta tcattgcagc actggggcca gatggtaagc
     2281 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2341 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2401 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2461 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2521 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2581 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2641 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2701 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2761 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2821 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2881 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2941 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3001 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3061 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3121 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcag
     3181 atcctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact tcttccgcat
     3241 caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt
     3301 attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg
     3361 gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa
     3421 tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat
     3481 gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc
     3541 cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca
     3601 gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact
     3661 ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtcttgaaca
     3721 ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa
     3781 gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc
     3841 gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc
     3901 gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac
     3961 cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa
     4021 caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt
     4081 gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac
     4141 gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc
     4201 cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg
     4261 gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac
     4321 gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta
     4381 cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga
     4441 ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg
     4501 cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga
     4561 gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa
     4621 acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg cctgatctgg
     4681 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     4741 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
     4801 cctgtcaaac actgatagtt taaaccactt cgtgcagaag acaattgcgg agagcggaga
     4861 attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg
     4921 aactgacaga accgcaacgt tgaaggagcc actcagccgc gggtttctgg agtttaatga
     4981 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
     5041 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
     5101 tccaattaga gtctcatatt cactctcaaa tgattgaaca agatggattg cacgcaggtt
     5161 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5221 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5281 ccgacctgtc cggtgccctg aatgaactcc aagacgaggc agcgcggcta tcgtggctgg
     5341 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5401 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5461 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5521 gcccattcga ccaccaagcg aaacatcgca tcgagcgagg acgtactcgg atggaagccg
     5581 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5641 tcgccaggct caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc cagggcgatg
     5701 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5761 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5821 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5881 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcttgt caagcagatc
     5941 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     6001 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     6061 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     6121 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     6181 tactagatcg acgctggatc cgaattcggc agggcccact gcatccaccc cagtacatta
     6241 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     6301 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     6361 atacaggcag cccatcagcc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     6421 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     6481 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     6541 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     6601 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     6661 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     6721 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     6781 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     6841 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     6901 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     6961 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     7021 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     7081 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     7141 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     7201 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     7261 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     7321 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     7381 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     7441 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     7501 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     7561 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     7621 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     7681 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
     7741 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     7801 caaccgccgg cagccggtgc gtcgtcgatt aggaagccgc ccaagggcga cgagcaacca
     7861 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     7921 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     7981 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
     8041 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     8101 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     8161 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     8221 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     8281 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     8341 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     8401 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     8461 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     8521 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     8581 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     8641 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     8701 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaagc
     8761 ttctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     8821 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     8881 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     8941 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
     9001 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
     9061 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
     9121 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccgagct cccatccccc
     9181 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggccaat
     9241 tcgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9301 tgagacaata accttgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
     9361 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgca aaagatacgg
     9421 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     9481 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     9541 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     9601 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     9661 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     9721 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     9781 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
     9841 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     9901 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     9961 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
    10021 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
    10081 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
    10141 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10201 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10261 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10321 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10381 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10441 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
    10501 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10561 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10621 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10681 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10741 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10801 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10861 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10921 cctttttacg gttcctgtgg ttggcacata caaatggacg aacggataaa ccttttcacg
    10981 cccttttaaa tatcskwttm wyytrwtwma mkytcktkts tykkattctg atttatctgt
    11041 tgttgatgtg attacagaat ggcaaattct aagcttgtgt tcagttctat ttttctagtc
    11101 ttaattattt tcaatttatg tttctcaacc cttggcagac ccctaatgaa aagagagagc
    11161 aaattggagt acgtatcagc atacgaaaac atagtcattt ggcggcgtaa cattttagag
    11221 aatgaagccg cgacaacatt agattcccag aatccacatg ttggcgtcga ggctgaaaaa
    11281 ttggttgacg attttcgacc aacggatcca ggtcatagtc ctggtgctgg ccattcaacc
    11341 ccaacaatcc ccatggacac caacatgcct caaggcgtta attaagcttc ycyrccaata
    11401 tatcctgtca aacactgata gtttaaactc cctcggtcac atgtgcatcc tctagtggag
    11461 ggagagagga ttttgaggaa ataattaatt gaattacttg tattattgat aaagtaattt
    11521 agataagttg ttagtacaac ttattgcaac tatggataga caaaaatcac ttattttaag
    11581 gggagaagta tataacaact tattataaaa tttctgagca gccggcctcc tccaatcaat
    11641 catagagagt ggagcccatt ctggaaaacc aaggaacccc caacttgcac tggtgcggtg
    11701 gcccaattca aaaaaaacgg ggcccagcaa agtaacgccg ttgtaacgat ttatcaatcc
    11761 aaactcaaaa ggcggcgaga tgcgtttaac tccgtgaaat taacaaaccg ccaacaactt
    11821 gcaatttgca actaccgttt ccaagaagaa ctcaaccaca caacgtatcc tatcccaaac
    11881 cacacgcaac tagtgacgcg tcataaggac acgtgtcaca atttgactgg ttaataattt
    11941 caccgctttt gctataaatt acctccaatc cccttagctt cttcacaatt cagttcccaa
    12001 ccctaacaat tcttgttcat atcgcttctc tctactttca aggtatgatc caatttctct
    12061 cttctttctc tgtaatcctt tcgttgagtt ttgtttccga tcaatcatag gtagttttct
    12121 tgtttcgaag catgagatct aggaattttt tgtgattttc caaaattgag atcggtttga
    12181 aattgaattt tacagcttga atctcagatc ttgttttatc aatgttttcg atggcttgcg
    12241 atgtagatct atgataattg tggttcagtt ttgttaggaa tcgatttcgg tttagcaatt
    12301 gcagattaat tagggtttcc aattgaattc ttcagatccg ttatggaatt atgtcaaata
    12361 atttattcaa attggaaatt attgttagat ccactcttaa tctgtttgat ccaagcttca
    12421 attagggttt tcacttgttt caatttcttg ttatggattc tgatttatct gttgatgtta
    12481 gatccactct taatctgttt gatccaagcg ttaattaggg ttttcacttg tttcaatttc
    12541 gtgtgttgga ttctgattta tctgttgttg atgtgattac agaatggcaa attctaagct
    12601 tgtgttcagt tctatttttc tagtcttaat tattttcaat ttatgtttct caacccttgg
    12661 cmgmsmcmwm atkagattcc cagaatccac atgttggcgt cgaggctgaa aaattggttg
    12721 acgattttcg accaacggat ccaggtcata gtcctggtgc tggccattca accccaacaa
    12781 tccccatgga caccaacatg cctcaaggcg ttaattaagc ttctctagct agagtcgatc
    12841 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
    12901 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
    12961 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
    13021 ccagatcgct aagcttgaat tcttgcggag agcggagaat taagggagtc acgttatgac
    13081 ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg
    13141 aaggagccac tcagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac
    13201 cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca
    13261 aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca
    13321 ctctcaaatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    13381 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    13441 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    13501 tgaactccaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    13561 agctgtgctc
//
