LOCUS       sequence-325513-       17243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17243)
  AUTHORS   Roy S, Torres-Jerez I, Zhang S, Liu W, Schiessl K, Jain D, Boschiero
            C, Lee HK, Krom N, Zhao PX, Murray JD, Oldroyd GED, Scheible WR, 
            Udvardi M
  TITLE     The peptide GOLVEN10 alters root development and noduletaxis in 
            Medicago truncatula.
  JOURNAL   Plant J. 2024 May;118(3):607-625. doi: 10.1111/tpj.16626. Epub 2024 
            Feb 15.
  PUBMED    38361340
REFERENCE   2  (bases 1 to 17243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16626";
            journalName: "Plant J"; date: "2024-05"; volume: "118"; issue: "3"; 
            pages: "607-625"
COMMENT     Sequence Label: LjUBQ:MtGLV2:T35s
FEATURES             Location/Qualifiers
     source          1..17243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      138..332
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(568..590)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    676..816
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     731..750
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(831..848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1002..1590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1082..1101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1836..2627)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    3148..3172
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3265..3517)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3715..4740)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      complement(5056..5308)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(5321..5995,5996..5998,5999..6001))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MARSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(5383..5406)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5581..5600)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5798..5818
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     misc_feature    13829..13853
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15184..15813
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(16242..17243,1..72)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
       61 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
      121 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
      181 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
      241 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
      301 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
      361 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
      421 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
      481 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
      541 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
      601 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      661 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
      721 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      781 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
      841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1441 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     1681 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     1741 tttggctgtg agcaattatg tgcttagtgc atctaacgct tgagttaagc cgcgccgcga
     1801 agcggcgtcg gcttgaacga atttctagct agacattatt tgccgactac cttggtgatc
     1861 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1921 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     1981 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     2041 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2101 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2161 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2221 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
     2281 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     2341 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
     2401 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     2461 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     2521 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     2581 acctctgata gttgagtcga tacttcggcg atcaccgctt cccccatgat gtttaacttt
     2641 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     2701 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa
     2761 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     2821 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag
     2881 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt
     2941 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     3001 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     3061 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     3121 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     3181 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     3241 gaattatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg cgcgataatt
     3301 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
     3361 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
     3421 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
     3481 atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag ggaattaatt
     3541 cctgaatcac tgcgaccggc cctcccgcga cccagccgag cgagcttagc gaactgtgga
     3601 cgagaactgt gccaccaagc gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc
     3661 gagttgctgg ctgaggcgtt ctcgaaatca gctcttgttc ggtcggcatc tactctattc
     3721 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     3781 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     3841 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     3901 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagcct
     3961 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     4021 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     4081 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     4141 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     4201 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     4261 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     4321 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatagc
     4381 atccatagcc tccgcgaccg gctgaagaac agcgggcagt tcggtttcag gcaggtcttg
     4441 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaactcccc
     4501 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     4561 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     4621 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     4681 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     4741 aggggggatt cgagttgaga gtgaatatga gactctaatt ggataccgaa tttatggaac
     4801 gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt
     4861 tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc
     4921 ggctcagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc
     4981 cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg atcgataatt
     5041 cgtcgacggc gcgccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
     5101 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
     5161 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
     5221 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
     5281 aactttattg ccaaatgttt gaacgatcgg gaattggatc ctacaggaac aggtggtggc
     5341 ggccctcggt gcgctcgtac tgctccacga tggtgtagtc ctcgttgtgg gaggtgatgt
     5401 ccagcttgga gtccacgtag tagtagccgg gcagctgcac gggcttcttg gccatgtaga
     5461 tggacttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc ttgtggatct
     5521 cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg
     5581 tcttcttctg cattacgggg ccgtcggagg ggaagttcac gccgatgaac ttcaccttgt
     5641 agatgaagca gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
     5701 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg
     5761 ggatgtcggc ggggtgcttc acgtacacct tggagccgta ctggaactgg ggggacagga
     5821 tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttcacg gtgttgtggc
     5881 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggtgc
     5941 cctccatgcg caccttgaag cgcatgaact ccttgatgac gttcttggag gagcgcgcca
     6001 tggcaaagat ctgcatctgt taatcagaaa aactcagatt aatcgacaaa ttcgatcgca
     6061 caaactagaa actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga
     6121 caaaactcaa attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa
     6181 gatgatatct aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa
     6241 accaaagaac atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa
     6301 taaggaacac agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga
     6361 ttttgagaaa ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag
     6421 aagaagaaga agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa
     6481 tagctaataa taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc
     6541 taattgcctt atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact
     6601 cgtgtgtgcc ggctgcaacc actttgacgc cgtttattac tgactcgtcg acaaccacaa
     6661 tttctaacgg tcgtcataag atccagccgt tgagatttaa cgatcgttac gatttatatt
     6721 tttttagcat tatcgtttta ttttttaaat atacggtgga gctgaaaatt ggcaataatt
     6781 gaaccgtggg tcccactgca ttgaagcgta tttcgtatct tctagaattc ttcgtgcttt
     6841 atttcttttc ctttttgttt ttttttgcca tttatctaat gcaagtgggc ttataaaatc
     6901 agtgaatttc ttggaaaagt aacttcttta tcgtataaca tattgtgaaa ttatccattt
     6961 cttttaattt tttagtgtta ttggatattt ttgtatgatt attgatttgc ataggataat
     7021 gacttttgta tcaagttggt gaacaagtct cgttaaaaaa ggcaagtggt ttggtgactc
     7081 gatttattct tgttatttaa ttcatatatc aatggatctt atttggggcc tggtccatat
     7141 ttaacactcg tgttcagtcc aatgaccaat aatatttttt cattaataac aatgtaacaa
     7201 gaatgataca caaaacattc tttgaataag ttcgctatga agaagggaac ttatccggtc
     7261 ctagatcatc agttcataca aacctccata gagttcaaca tcttaaacaa gaatatcctg
     7321 atctgaagaa tgtggaggct ttagtccctt ggatacttgg gaggctgtgg aagaacagaa
     7381 acgagctggt gcttaaaggg agggaatttg gaaccaatga ggtattagta aggacacaag
     7441 aagatgcaga tgagtggatt agaaggaaag aggctcagaa tgtaaggaaa gcaccaacca
     7501 caacggcgcg cccgggagag aggattttga ggaaataatt aattgaatta cttgtattat
     7561 tgataaagta atttagataa gttgttagta caacttattg caactatgga tagacaaaaa
     7621 tcacttattt taaggggaga agtatataac aacttattat aaaatttctg agcagccggc
     7681 ctcctccaat caatcataga gagtggagcc cattctggaa aaccaaggaa cccccaactt
     7741 gcactggtgc ggtggcccaa ttcaaaaaaa acggggccca gcaaagtaac gccgttgtaa
     7801 cgatttatca atccaaactc aaaaggcggc gagatgcgtt taactccgtg aaattaacaa
     7861 accgccaaca acttgcaatt tgcaactacc gtttccaaga agaactcaac cacacaacgt
     7921 atcctatccc aaaccacacg caactagtga cgcgtcataa ggacacgtgt cacaatttga
     7981 ctggttaata atttcaccgc ttttgctata aattacctcc aatcccctta gcttcttcac
     8041 aattcagttc ccaaccctaa caattcttgt tcatatcgct tctctctact ttcaaggtat
     8101 gatccaattt ctctcttctt tctctgtaat cctttcgttg agttttgttt ccgatcaatc
     8161 ataggtagtt ttcttgtttc gaagcatgag atctaggaat tttttgtgat tttccaaaat
     8221 tgagatcggt ttgaaattga attttacagc ttgaatctca gatcttgttt tatcaatgtt
     8281 ttcgatggct tgcgatgtag atctatgata attgtggttc agttttgtta ggaatcgatt
     8341 tcggtttagc aattgcagat taattagggt ttccaattga attcttcaga tccgttatgg
     8401 aattatgtca aataatttat tcaaattgga aattattgtt agatccactc ttaatctgtt
     8461 tgatccaagc ttcaattagg gttttcactt gtttcaattt cttgttatgg attctgattt
     8521 atctgttgat gttagatcca cttttaatct gtttgatcca agcgttaatt agggttttca
     8581 cttgtttcaa tttcgtgtgt tggattctga tttatctgtt gttgatgtga ttacagatgg
     8641 cttctcttcc tcttcttctg ctcctcatcc tctgtggttc tttgcatgca tgcacccctc
     8701 gtccttttac tgaaaaagac acaacgaaat accatttcaa taaggttgga ataaatatat
     8761 ttatagtcct tataactatc tacaaatttg tttttagatc cttctatcta ttttttcggt
     8821 cactacaaat tacaaaattg catgtagttt tgtaaatttt aaccactgaa aaatgagtat
     8881 atgaataaaa tataaatttt gtaaagaata aaaatatatt aactttttta atatgtaata
     8941 ttcttttgat tattttgtat catattattc atttgaaatt attatttttg actaaaattt
     9001 gtgattatat ttaggaatta aacaatgtta aattgttgga gacgttaact acatcaagtg
     9061 taaaggataa taagctagag gagaatccag cactgcaaca gaaagtcaca aatgatgaaa
     9121 taaacatttg tgtgagctgc agcagtgcat caactttgaa gaaagtgatc gtaggtacat
     9181 aattttctat cacccttttt gattgttgtt agttgttgtg tatactattt ttattatttt
     9241 tttggacaga agttattgtg tattatacgt actatttaaa gtgcaattta ccccataatt
     9301 atttgtccct tgcctttgtt ttctatgcaa ataaaatctg acttttgtta acaggtgagg
     9361 ctcatacata atctatggtg cacttggttg tatgtcattt tattactaaa ttattgctaa
     9421 ggaattttgt agctttatgt aaattaattg tttagaaatt ttgcattggc tcgatgcatc
     9481 caataattta tgcttattgt aataaaaata ataagtgatg gtggtcgata tgcaattgca
     9541 gggtcaacgt tccaaactgg tacaccatcc cttcttgcag caaaggtaat ataaagacaa
     9601 gtgaagttca atattatttg agtatacttg cattctgccc aaaaaaaata aaattgagta
     9661 tacttgcaat ctagacattt aaatcttttt atatatccat ctaatagaac tacattataa
     9721 ttatccccgt aagcttagct cagttggtag agatattgca ttttatatgc agagatcggg
     9781 ttcgaacccc agacacttta cttgtccaca attaaattgt gtgagctcta accattagac
     9841 tacttaaaaa aaagaaacta catcataatt gatcaccaaa agaaaaataa ttacattgta
     9901 atttaatatt agtgttaatt attaaaaata tatttacatt atatacatat ttactttcat
     9961 tgaattctaa aatacagtat gagaattaag atatgaactt aaaagatatg catgttatcg
    10021 tgaaacagat ttgcattgaa aaaatccact tgagttggtt tagaacctga aagtgcactc
    10081 ctctttgatg gctggaaaat cctctatttt gcataatacc ccactcctta tggttatttt
    10141 gatttgtttt gagaaactac attattttga attttgaaat ggttattttg aaattggttg
    10201 tgtgggatag aaaaatagtg gtctttcatt ggatgtcaaa gtgttttatt tttttatttt
    10261 tttaacaaca aacaaaatat ttctactgtt attttgaaag acattttaag aaacggtata
    10321 gtatatacaa atcaaatcaa atggaatata accaggtgtc tgactcaact tattaaacat
    10381 tttttgttat ggctcaattg attgagagta ctcaaatttg agccgttgat gatatactta
    10441 ttggttaaac tttatttaga acctgttgaa acacttccta aatttttttt tttttagttt
    10501 ttaaaatttt gaaaattaaa aacttttttg ctaaactatt tttttaaatt acagttttgg
    10561 aaactgtttt gactttttta gtttcgagga ttaaatctaa aacatgtaaa aagataacta
    10621 ttgtgaaaat agaacttgta tatatatttc aaaaactgaa aacaaaaact atttcaaaca
    10681 tgctcttaat tattactttt caatattcta gattgttcga aatgttttcc tagaaatcaa
    10741 aattttggcc cttaaaagga aatcctttta ataatacagt agaggtaggt aggtttgtaa
    10801 attacatgca tctattcacg ttgtctaaaa tgagcaattc actttaggtg taaagtgaat
    10861 aatatcaatt ttcaataaac aaatgagtgg tatatattaa atgcatatgt ataacaaacc
    10921 attgctgatc aaaatcctaa ccacttattt tagagaagtc aaatttgtcg gggataatag
    10981 tcattttggt ccaggatagg ttttagtacg aggagttcca tacaataaaa ggatttctct
    11041 ataataagag tgatccatgg ccttgagcct agagtcatag aagaattcct taatgcttcc
    11101 catagggata ggaaagagta aagtggccgg ttaagcagct agtcctaatc taggcacata
    11161 tgccggttag agcagtgcct tttcaaagct tagcttctga gattctctta ctaatagaaa
    11221 acaatctttc tttcgggaaa gtcttctgcg ctctattagg ctgtggggcc cagatctttt
    11281 tgaataaact actgtttgcc ccttggaatg aagcctctta ggctgtctac ctttgattca
    11341 agagtctcac cttggcgatc tactgagaag gtcgtatcca tggtctcgac tcgctcatac
    11401 ccggcccacc tctctgaggg ggacttcctt gctatgccac catcctatta gcttagctgg
    11461 tagttaatct atttactagt ctgatgcaca cttggaaggc tctcctttat cttacctgtc
    11521 gtgcagcaca atacacgcgt ttaggtcata attcttatct aaggctgata attgggctta
    11581 taatgccttt gttttatgga cctcgggacc tttcatttcc aacttcggcc tatcatctta
    11641 tgtttttagt ttaaaaaata taaaaggttc gcgccgcatc cacgtcttag ccttaacgga
    11701 tgaggcctgc tatgacaagg gatagatagg cactacctct aacaaggtag tcggcgatat
    11761 aatgccttct ggatacgact caagaataaa ccattatcta tcttcctagt ccctttgtct
    11821 gagcacaggt gactctctta gtgttagtac ttgttcagtt aagatgtaca tagtctttag
    11881 ggtacggtca ttacgcaaca atatatcttc cccactgggc gcgtatggta agatgagata
    11941 catcctctgg gcctaagtcg atccttaaag actttctttg atctggattc agaggcttca
    12001 tctaagtcta agtagagtga aacatgatcc tgccctcctc ttactctttg ttacagctat
    12061 ttcctcgtct gggaagcaat ccttttgcta tttattaaaa aatattatat ttaaccaagt
    12121 taaatgttag caatatattt tagtatacat ttttatgttt cgatgcaaag tcgttttttg
    12181 gttggttaat tggtccatgt atgtttaata agtagtcaca atagtaattc aatatatcaa
    12241 aattttaaaa tagtcattga ttatgtactt cgtcagtaaa aaataatctc tcacattaaa
    12301 aagtctatca acgttgacat taactctttt catatactca atccgtttct aaatataagc
    12361 aaaattgatt ttttaggttc attcatttaa tgatgtatgt ggtccatata atgaaccaca
    12421 tacatcatta aataagtgga tcaaaatgaa tattaccctg gataaatttg acaataagta
    12481 agtatttaca tggactgata taaccataag taagtatgtt gagggactaa tattacaata
    12541 ttaatgaaac attttaacat ggggaattat tttgactaac ttagtataca atcagagact
    12601 attttgatat ttcggcacat tgaaggacaa ttgtggctaa tcacgttaca cattcagaaa
    12661 ctaaaatgac tattatccca atttatgtat tataatacct agatgatcat aatatgcttt
    12721 ggtttactat tgaacataaa ttcgattaaa atttgaaaac tcaactttaa actaacataa
    12781 ttttggagag ttttgccaaa gaaatatcta atgcttatgc aatttatgag tttgtctaat
    12841 atgaacctct caaaaatgag tctaaaggta aacctatcat ttttaataag aaaaataatt
    12901 ttataacaat aataaacgaa aagcaaatgt catgttttat tataggtgag gttaggttgc
    12961 ctttgaagaa ttgtggattt gatctatcaa ttaatcaaaa ttagctaaat gagtaaatat
    13021 gcaagtggtt ctacaaacta atttccactt tatttataaa tttgcttatg tgactaattt
    13081 tgattggttg agtggtaaat tactcggaat tcatcaaaat aacttaagac aaaaccttta
    13141 ctatactaga aatagtaaga ttcccctttg aatcatgtta atattaaagg ccatctgctt
    13201 tcatttaaaa ttgagattgc atatgccaat tgtaaaccaa gactagagcc actttcagtt
    13261 ttactgtgcc gacgtaaaga aactccaaaa aaaattaaca tggtgtgata taatattata
    13321 catatatacg tgtttgttgg tttagaaata tgatttgaat gatagaaaac gatgagacat
    13381 ggttgtgcag gatgaaaggg gacatgcaag gtcaatgctg ggaccagcta cgcaccatgt
    13441 tgaagaaaca atggttataa acaccaatga gaatgctgag gacatagttg aggattatgc
    13501 acaacctcat cggaaagcac ctattcacaa tgaaaagcca tgacggccat gctagagtcc
    13561 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
    13621 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
    13681 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
    13741 taattcctaa aaccaaaatc cagtgaaagc ttagcttgag cttggatcag attgtcgttt
    13801 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
    13861 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
    13921 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt
    13981 actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
    14041 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
    14101 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
    14161 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
    14221 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
    14281 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
    14341 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
    14401 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
    14461 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
    14521 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
    14581 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
    14641 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
    14701 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
    14761 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
    14821 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
    14881 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
    14941 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
    15001 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
    15061 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
    15121 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
    15181 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
    15241 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
    15301 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
    15361 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
    15421 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
    15481 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
    15541 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
    15601 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
    15661 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
    15721 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
    15781 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
    15841 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
    15901 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
    15961 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
    16021 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
    16081 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
    16141 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
    16201 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
    16261 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
    16321 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
    16381 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
    16441 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
    16501 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
    16561 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
    16621 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
    16681 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
    16741 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
    16801 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
    16861 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
    16921 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
    16981 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
    17041 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
    17101 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
    17161 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
    17221 agtacgattt gaaggaggag gcg
//
