LOCUS       sequence-325514-       13416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtIDA10:T35s
FEATURES             Location/Qualifiers
     source          1..13416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      255..507
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    618..642
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      734..1365
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1452..1556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1557..1625,1626..2417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1775..1794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2588..3176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3512..4660
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4816..4840
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4962..5141
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5163..5957
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      5970..6222
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6317..6341
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6605..7234
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7663..8736
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8802..8996
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9279..9383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9384..10178
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10349..10937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    11060..11084
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     polyA_signal    12586..12760
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        12860..13039
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tcgtgaccca gggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
       61 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
      121 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
      181 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
      241 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
      301 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
      361 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
      421 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
      481 gcgcggtgtc atctatgtta ctagatcgac gctggatccg aattcggcat tgtcttcaca
      541 gagtggggcc cactgcatcc accccagtac attaaaaacg tccgcaatgt gttattaagt
      601 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
      661 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtcagatca
      721 ggatctcctt tgcgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
      781 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc
      841 ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg
      901 acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc
      961 ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga
     1021 gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga
     1081 ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg
     1141 acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca
     1201 tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta
     1261 aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca
     1321 cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca
     1381 tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg
     1441 gccaattcgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1501 cgctcatgag acaataacct tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1561 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1621 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1681 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1741 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1801 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1861 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1921 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1981 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2041 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2101 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2161 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2221 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggttctcgcg
     2281 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2341 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2401 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2461 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2521 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2581 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2641 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2701 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2761 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2821 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2881 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2941 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3001 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3061 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3121 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3181 ccagcaacgc ggccttttta cggttcctgg cagatcctag atgtggcgca acgatgccgg
     3241 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     3301 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     3361 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     3421 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     3481 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
     3541 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     3601 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     3661 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     3721 ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat
     3781 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     3841 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     3901 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     3961 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     4021 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     4081 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     4141 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     4201 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     4261 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     4321 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     4381 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     4441 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     4501 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     4561 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     4621 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     4681 ggctgggggt tcagcagcca gcgcctgatc tggggaaccc tgtggttggc atgcacatac
     4741 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     4801 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaacca
     4861 cttcgtgcag aagacaattg cggagagcgg agaattaagg gagtcacgtt atgacccccg
     4921 ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga
     4981 gccactcagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta
     5041 ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat
     5101 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
     5161 aaatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5221 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5281 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     5341 tccaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5401 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5461 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5521 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5581 gcatcgagcg aggacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5641 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cggatgcccg
     5701 acggcgagga tctcgtcgtg acccagggcg atgcctgctt gccgaatatc atggtggaaa
     5761 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5821 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5881 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5941 ttgacgagtt cttctgagct tgtcaagcag atcgttcaaa catttggcaa taaagtttct
     6001 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     6061 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     6121 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     6181 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgacgctgg atccgaattc
     6241 ggcagggccc actgcatcca ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     6301 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     6361 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gccgtgcggc
     6421 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     6481 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     6541 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     6601 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     6661 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     6721 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     6781 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     6841 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     6901 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     6961 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     7021 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     7081 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     7141 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     7201 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     7261 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     7321 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     7381 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     7441 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     7501 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     7561 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     7621 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     7681 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     7741 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     7801 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgtcgtcg
     7861 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     7921 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     7981 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     8041 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     8101 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     8161 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     8221 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     8281 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     8341 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     8401 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     8461 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     8521 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     8581 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     8641 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     8701 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     8761 attgccctag caggggaaaa aggtcgaaaa agcttctttc ctgtggatag cacgtacatt
     8821 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     8881 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     8941 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     9001 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     9061 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     9121 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     9181 cgcccacatc aaggcaccga gctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
     9241 ggcctcaccc caaaaatggc agcgctggcc aattcgtgcg cggaacccct atttgtttat
     9301 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccttga taaatgcttc
     9361 aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac
     9421 tgatcgaaaa ataccgctgc gcaaaagata cggaaggaat gtctcctgct aaggtatata
     9481 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     9541 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     9601 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     9661 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     9721 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     9781 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     9841 atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg
     9901 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     9961 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
    10021 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
    10081 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
    10141 aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat
    10201 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10261 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10321 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10381 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10441 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    10501 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10561 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10621 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10681 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10741 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10801 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10861 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10921 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg tggttggcac
    10981 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    11041 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    11101 actccctcgg tcacatgtgc atcctctagt ggagggagag aggattttga ggaaataatt
    11161 aattgaatta cttgtattat tgataaagta atttagataa gttgttagta caacttattg
    11221 caactatgga tagacaaaaa tcacttattt taaggggaga agtatataac aacttattat
    11281 aaaatttctg agcagccggc ctcctccaat caatcataga gagtggagcc cattctggaa
    11341 aaccaaggaa cccccaactt gcactggtgc ggtggcccaa ttcaaaaaaa acggggccca
    11401 gcaaagtaac gccgttgtaa cgatttatca atccaaactc aaaaggcggc gagatgcgtt
    11461 taactccgtg aaattaacaa accgccaaca acttgcaatt tgcaactacc gtttccaaga
    11521 agaactcaac cacacaacgt atcctatccc aaaccacacg caactagtga cgcgtcataa
    11581 ggacacgtgt cacaatttga ctggttaata atttcaccgc ttttgctata aattacctcc
    11641 aatcccctta gcttcttcac aattcagttc ccaaccctaa caattcttgt tcatatcgct
    11701 tctctctact ttcaaggtat gatccaattt ctctcttctt tctctgtaat cctttcgttg
    11761 agttttgttt ccgatcaatc ataggtagtt ttcttgtttc gaagcatgag atctaggaat
    11821 tttttgtgat tttccaaaat tgagatcggt ttgaaattga attttacagc ttgaatctca
    11881 gatcttgttt tatcaatgtt ttcgatggct tgcgatgtag atctatgata attgtggttc
    11941 agttttgtta ggaatcgatt tcggtttagc aattgcagat taattagggt ttccaattga
    12001 attcttcaga tccgttatgg aattatgtca aataatttat tcaaattgga aattattgtt
    12061 agatccactc ttaatctgtt tgatccaagc ttcaattagg gttttcactt gtttcaattt
    12121 cttgttatgg attctgattt atctgttgat gttagatcca ctcttaatct gtttgatcca
    12181 agcgttaatt agggttttca cttgtttcaa tttcgtgtgt tggattctga tttatctgtt
    12241 gttgatgtga ttacagaatg ccacagaaaa agcagaacct ccaactagca gtagctctgc
    12301 tcattctatt atcattaata tcatcttcat catctgctag gcccttgaat gatttcagcc
    12361 agcttgcaga aacctcaaac atcgcacttc caagtgagag ggcaatgccg gaggagaagg
    12421 aaggacacat atcagtagta cgggaaagag gcggtgcaaa atcatatggg ccattgatcc
    12481 tgaacatgct ccctaagggg aaagttcctt catctggacc aagcaaaggg accaacaacc
    12541 tcaacaacta ggcttctcta gctagagtcg atcgacaagc tcgagtttct ccataataat
    12601 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
    12661 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
    12721 ctaattccta aaaccaaaat ccagtactaa aatccagatc gctaagcttg aattcttgcg
    12781 gagagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
    12841 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
    12901 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
    12961 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
    13021 tcccctcggt atccaattag agtctcatat tcactctcaa atgattgaac aagatggatt
    13081 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
    13141 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
    13201 ttttgtcaag accgacctgt ccggtgccct gaatgaactc caagacgagg cagcgcggct
    13261 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
    13321 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
    13381 tgctcctgcc gagaaagtat ccatcatggc tgatgc
//
