LOCUS       sequence-32553-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_FUS_G2A_R521H
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             170..196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    212..236
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             643..654
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgagctcgga tcgatccatc gatagtgtgg
      121 tggaattctg atcgccgccg ccatggacta caaggacgac gatgacaagt acccttatga
      181 cgtgcccgat tacgctttga accagatatc aacaagtttg tacaaaaaag caggctccac
      241 catgggaacc aattcagtcg acatggcctc aaacgattat acccaacaag caacccaaag
      301 ctatggggcc taccccaccc agcccgggca gggctattcc cagcagagca gtcagcccta
      361 cggacagcag agttacagtg gttatagcca gtccacggac acttcaggat atggccagag
      421 cagctattct tcttatggcc agagccagaa cacaggctat ggaactcagt caactcccca
      481 gggatatggc tcgactggcg gctatggcag tagccagagc tcccaatcgt cttacgggca
      541 gcagtcctcc taccctggct atggccagca gccagctccc agcagcacct cgggaagtta
      601 cggtagcagt tctcagagca gcagctatgg gcagccccag agtgggagct acagccagca
      661 gcctagctat ggtggacagc agcaaagcta tggacagcag caaagctata atccccctca
      721 gggctatgga cagcagaacc agtacaacag cagcagtggt ggtggaggtg gaggtggagg
      781 tggaggtaac tatggccaag atcaatcctc catgagtagt ggtggtggca gtggtggcgg
      841 ttatggcaat caagaccaga gtggtggagg tggcagcggt ggctatggac agcaggaccg
      901 tggaggccgc ggcagggtgg cagtggtggc ggcggcggcg gcggcggt
//
