LOCUS       sequence-325548-       13529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged FL mouse talin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard
            T
  TITLE     A distinct talin2 structure directs isoform specificity in cell 
            adhesion.
  JOURNAL   J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 
            10.1074/jbc.RA119.010789. Epub 2020 Jun 30.
  PUBMED    32605925
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010789"; journalName: "J Biol Chem"; date: 
            "2020-09-11- 11"; volume: "295"; issue: "37"; pages: "12885-12899"
COMMENT     Sequence Label: pCDNA_GFP-mTLN1-FL
FEATURES             Location/Qualifiers
     source          1..13529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             385..414
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(415..417,418..420,421..1131)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(415..417,418..420,421..1131)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(460..481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(721..740)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1068..1089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1138..1161
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8838..8867
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8883..8900
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8923..8940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8929..9153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9199..9627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9286..9305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9496..9517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9636..9656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9641..9970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9821..9956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9883..9902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10018..10065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10084..10458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10588..10709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10625..10644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10679..10698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10758..10774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10758..10774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10771..10793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10782..10798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10806..10812,10813..10830,10831..10836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10851..10872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10989..11006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11160..11748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11240..11259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11919..12710,12711..12779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12542..12561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12780..12884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12959..12978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13150..13529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg ctagcggtac catgcatcac caccaccatc atcatcacca ccatatggtg
      421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      481 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      601 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      841 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      901 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1021 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1081 gagttcgtga ccgccgccgg gatcaccctc ggcatggacg agctgtacaa gggatccctg
     1141 gaagttctgt tccaggggcc cgccgccgct atggttgcgc tttcgctgaa gattagcatt
     1201 gggaatgtgg tgaagacgat gcaatttgag ccatctacca tggtgtacga cgcctgccgc
     1261 atgattcgtg agcggatccc agaggccctg gctggccctc ccaacgactt tgggctcttt
     1321 ctgtcagatg atgaccccaa aaaaggcatc tggctagagg ctgggaaagc tttggactac
     1381 tacatgctcc gaaatgggga caccatggag tacagaaaaa agcagagacc cctgaagatc
     1441 cggatgttag acggaacggt gaagactatc atggtggatg actccaagac cgtcacagac
     1501 atgctcatga ccatttgtgc ccgaattggt atcaccaacc atgatgagta ttcactggtt
     1561 cgagagctga tggaagaaaa gaaagatgag gggacaggga ccctgagaaa agacaagacc
     1621 ctactgcgag atgaaaagaa gatggagaag ctaaagcaga agttgcacac agacgatgag
     1681 ttgaactggc tggaccatgg gcggacactg agggaacagg gagtggaaga gcatgagacg
     1741 ttgctgctgc ggagaaagtt cttctactca gaccagaatg tggattcccg agaccctgta
     1801 cagctgaacc ttctctatgt gcaggcacga gatgacatcc tgaatggctc ccatcctgtc
     1861 tcctttgaca aggcctgtga atttgcaggc ttccagtgcc agatccagtt tggacctcac
     1921 aatgaacaga agcacaaggc tggcttcctt gacctgaagg acttcctgcc caaggagtat
     1981 gtgaagcaga agggagagcg taagatcttt caggcacaca agaattgtgg gcagatgagt
     2041 gaaattgagg ccaaggtacg ctacgtgaag ctggcccgtt ccctcaagac ttacggtgtc
     2101 tccttcttcc tagtcaagga aaagatgaag gggaagaata aactggtgcc caggctgttg
     2161 ggcatcacta aggagtgtgt gatgcgtgtg gatgagaaga ccaaggaggt gattcaggaa
     2221 tggagcctca ccaacatcaa acgctgggct gcctctccca agagctttac tctggacttt
     2281 ggagactatc aggatggcta ctactcagta cagacgacag aaggcgagca gatcgcacag
     2341 ctcattgctg gctacataga tatcatcctt aagaagaaaa aaagcaagga ccattttggg
     2401 ctggagggag atgaagagtc tactatgttg gaggactcag tgtctcccaa aaagtcaaca
     2461 gtccttcagc agcagtacaa ccgagtgggg aaagtggagc acggctctgt ggctctgcca
     2521 gccatcatgc gctctggagc ctctggtcct gagaatttcc aggtggggag catgccacct
     2581 gcccagcagc agatcaccag tggccagatg caccgaggac acatgccacc tctgacttca
     2641 gcccagcagg cgctcactgg aaccattaac tccagcatgc aggctgtgca ggctgcccag
     2701 gccactctgg atgactttga gactctaccc cctcttggcc aggatgctgc ctccaaagct
     2761 tggcgtaaga acaagatgga tgaatcaaag catgagatcc actcccaggt agatgccatc
     2821 acagctggca ctgcctctgt ggtaaacctg acagcaggag atcctgcaga gacagactat
     2881 acagcagtgg gctgtgcagt caccaccatc tcttccaacc tgacggagat gtcccgtggg
     2941 gtgaagctat tggctgccct gctggaggat gaaggcggca atggccggcc ccttctgcaa
     3001 gcagcaaagg gccttgcagg ggctgtgtcg gaactgcttc gcagtgctca gcccgccagc
     3061 gctgagcctc gtcagaacct gctgctggca gccgggaacg tgggccaggc cagtggggag
     3121 ctgttgcagc aaattgggga aagtgacact gacccccact tccaggatgt tctaatgcag
     3181 ctagcaaacg cagtggcaag tgctgcagcc gccctggtcc tcaaggccaa gagtgtggcc
     3241 cagcgcacag aggattccgg gcttcagacc caagtgattg ctgcagctac gcaatgtgct
     3301 ctgtccactt cccagctggt ggcctgtacc aaggtggtag cacctacaat cagctcacct
     3361 gtgtgccaag aacagctagt cgaggctggg cgactggtgg ccaaagctgt ggagggctgt
     3421 gtgtctgcct cgcaagcagc tacagaagat ggacagctgc ttcgaggggt aggagcagca
     3481 gccacggctg tcacccaggc cctcaatgag ctgctgcagc acgtgaaggc ccacgccacc
     3541 ggagctgggc ctgccggtcg gtatgaccaa gccactgata ccatcctcac cgtcactgaa
     3601 aacatcttca gctccatggg tgatgctggg gagatggtgc gccaggcccg catcctggcc
     3661 caagccacct cagacctggt caatgctatc aaggccgatg ctgaggggga gagtgatctg
     3721 gagaactcta gaaagctcct gagtgctgcc aagatcctcg ctgatgccac cgccaagatg
     3781 gtggaggcgg ccaagggagc agccgcccac cctgacagtg aggaacagca gcagcgactg
     3841 cgtgaagcag ctgaggggct tcgcatggcc accaatgcag ctgcgcagaa cgccatcaag
     3901 aagaagttgg tgcagcgcct ggagcatgca gccaagcaag ctgcagcctc tgcaacacag
     3961 accattgctg cagcccaaca tgcagcctct gcccccaagg cctctgcggg cccccagccc
     4021 ctactggtgc agagctgtaa ggccgtggca gagcagattc cgttgctggt gcagggtgtc
     4081 cgaggaagtc aagctcagcc tgacagccct agtgctcagc tcgccctcat tgctgccagc
     4141 cagagcttcc tgcagccagg tgggaagatg gtggcagcgg caaaagcctc agtgccaaca
     4201 attcaggacc aggcttcagc gatgcagctg agtcagtgcg caaagaacct aggcactgcc
     4261 ctggccgagc tgcgcactgc tgctcagaag gctcaggagg catgtggacc tttggagatg
     4321 gattctgcac tgagtgttgt acaaaatcta gagaaagacc tacaggaaat aaaagcagca
     4381 gctcgagatg gcaagcttaa gcccttacct ggggaaacaa tggagaagtg tacccaagat
     4441 cttggcaaca gcaccaaagc ggtgagctct gccatcgcca agctgctggg ggagattgcc
     4501 cagggcaatg agaactatgc aggtattgca gctcgggatg tagctggtgg actaaggtca
     4561 ctagcccagg ctgcgcgggg tgtggctgcg ctgacatcag atcctgcagt gcaggccatt
     4621 gtgcttgaca cagccagcga cgttctggac aaggccagca gcctcattga agaggcaaaa
     4681 aaagcatctg gacacccagg ggacccagaa agccagcaaa ggcttgctca ggtagctaaa
     4741 gcagtgaccc aagccctgaa ccgctgtgtc agctgcctgc ctggccaaag agacgtggat
     4801 aatgccctac gagccgttgg agatgccagc aagcgactcc tgagtgactt gcttcctcca
     4861 agcacgggga catttcaaga agcacagagc cgattgaatg aagctgcagc tgggttaaat
     4921 caggccgcca cagaactggt gcaagcctcc cgaggaactc ctcaggacct ggctcgggcc
     4981 tccggccgat ttggacagga cttcagcacc ttcctggaag ctggtgtgga gatggccgga
     5041 caggccccga gccaggagga ccgagcccag gtggtgtcca acctgaaggg catatccatg
     5101 tcttcaagca aacttctcct tgccgctaag gccttgtcca cagaccctgc ttctcccaac
     5161 ctcaagagtc agctggctgc ggctgcccgg gcagtgacgg acagcatcaa ccagctcatc
     5221 accatgtgca cccagcaggc acctggccag aaggagtgtg acaatgcact tcggcagcta
     5281 gagacagtcc gagaactcct ggagaatcca gtccagccca tcaacgacat gtcctacttc
     5341 ggttgcttgg acagtgtcat ggagaactcc aaggtcctag gtgaggccat gactggcatc
     5401 tcccaaaatg ccaagaatgg aaatctgccg gagtttggag atgccattgc cacagcctcc
     5461 aaggctctct gtggcttcac ggaggcagcc gcacaggcag catatctagt tggtgtctct
     5521 gaccccaaca gccaagctgg acagcaagga ctggtggaac ccacacagtt tgcccgtgca
     5581 aaccaggcaa ttcagatggc ctgtcagagt ctgggggagc ctggctgtac ccaggcccag
     5641 gtgttatctg cagccactat tgtagccaaa cacacatctg cattgtgtaa cagctgtcgc
     5701 ctggcttccg ctaggactgc caatcccact gccaagcgcc agtttgtaca gtcagccaag
     5761 gaggtggcca acagtacagc caatcttgtc aagaccatca aggcactaga tggggacttc
     5821 acagaagaga accgtgccca gtgccgagca gccacagccc ctctgctgga agctgtggat
     5881 aacctgagtg cctttgcctc caaccctgag ttctccagcg tccctgccca gatcagccct
     5941 gagggcagag cagccatgga gcccattgta atctctgcta agacaatgtt ggagagtgct
     6001 ggagggctca tccagacagc tcgggcgtta gcagtcaatc cccgagaccc cccacgttgg
     6061 tctgtgctag ctggccactc tcggactgtc tcagattcca tcaagaaact tattacaagc
     6121 atgagggaca aagccccagg gcagctggag tgtgagacag ccattgcggc tctgaacagc
     6181 tgcttgcggg acctagatca ggcttcgctt gccgctgtca gccagcagct tgctccccgt
     6241 gaaggaatct ctcaagaggc tttgcacacc cagatgctca ctgcagtgca agaaatttct
     6301 catctcattg agccgctggc cagcgctgct cgggctgaag cctcccagct gggacacaag
     6361 gtgtcccaaa tggcccagta ctttgaacca ctcaccctgg ctgcagtggg tgctgcgtct
     6421 aagaccctga gccacccgca gcagatggca cttctggacc agactaaaac gttggcagag
     6481 tctgccttgc agttgctata tactgccaag gaggctggtg gtaaccccaa gcaagcagca
     6541 cacacccagg aagccctgga ggaggctgtg cagatgatga cagaggccgt agaagacctg
     6601 acgacgaccc tcaacgaagc agccagtgct gcaggagtcg ttggcggcat ggtggactct
     6661 atcactcagg ccatcaacca gctagatgaa ggacctatgg gtgacccaga aggctcattc
     6721 gtagattacc agacaaccat ggtgaggaca gccaaggcca ttgctgtcac tgttcaggag
     6781 atggtaacca agtcaaacac cagccctgaa gagctaggcc ctcttgccaa ccagctgacc
     6841 agtgactatg gccgactggc ctcacaagcc aagcctgcag ctgtggctgc tgaaaatgaa
     6901 gagataggcg ctcacatcaa acaccgagta caggagctgg gccatggctg ctctgctctg
     6961 gtcaccaagg caggtgcctt gcagtgtagc cccagtgatg tctacaccaa gaaggagctc
     7021 atagagtgtg cccgcagagt gtcagagaag gtctcccatg ttctggctgc actccaggct
     7081 gggaatcgtg gtacccaggc ctgcattaca gcagccagtg ctgtgtctgg tatcattgct
     7141 gacctcgaca ccaccatcat gttcgctact gctggcacac ttaaccgtga gggtgctgaa
     7201 acttttgctg accaccggga gggtatctta aagacagcaa aggttcttgt ggaggacacc
     7261 aaggtcctag tgcagaatgc agctgggagc caggagaagt tggcacaagc cgcccagtcc
     7321 tccgtggcca ccattacccg cctcgctgat gtggtcaagc tcggtgcagc cagcctagga
     7381 gccgaagacc ctgaaactca ggtggtgctg atcaatgcag taaaggacgt agccaaggcc
     7441 ctgggtgacc tcatcagcgc tacgaaggct gcagcgggca aagttgggga tgaccctgca
     7501 gtgtggcagc tcaagaactc tgccaaggtg atggtgacca atgtgacatc attgctcaag
     7561 acagtgaagg ctgtggaaga tgaggccacc aaaggcacac gggccctaga ggcaaccaca
     7621 gagcacatac gtcaggaact ggcggtcttc tgttccccag agccacctgc caagacctct
     7681 acccctgaag acttcatccg aatgaccaag ggtattacta tggcaacagc caaagccgtt
     7741 gctgctggca attcctgtcg acaggaagat gtcattgcca cagccaatct gagccgacgg
     7801 gctattgcgg acatgcttcg ggcttgcaag gaagcagctt tccacccaga agtggcgcct
     7861 gatgtacggc tccgagccct gcactatggc cgggagtgtg ccaatggtta cctggaactg
     7921 ctggaccacg tgctgctgac ccttcagaag ccaaacccag acctgaagca gcagctaact
     7981 ggacactcaa agcgggttgc tggctctgtg actgagctca tccaggctgc tgaagccatg
     8041 aaaggaacag agtgggtgga cccagaggac cctactgtca ttgctgagaa tgagctcttg
     8101 ggagccgcag ccgccatcga ggctgcagcc aagaagctgg agcagctgaa gcccagggcc
     8161 aaacccaagg aggcggatga gtccttgaac tttgaggaac aaatcctaga agctgtcaag
     8221 tccatcgctg cagccaccag tgcactggta aaggctgcgt cagcagccca gagggagctg
     8281 gtggctcaag gaaaggtggg cgccattcca gccaatgcac tggatgatgg gcagtggtcg
     8341 cagggcctca tttccgctgc ccgtatggtg gctgcggcca ccaacaatct gtgtgaagca
     8401 gctaacgcag ctgtccaggg ccatgctagc caggagaaac tcatttcctc agccaagcaa
     8461 gtagctgcct ccacagctca gctcctggta gcttgcaagg tcaaagcaga tcaggactct
     8521 gaggcaatga aacggctcca ggctgctggc aatgcagtga agagggcctc agataacctg
     8581 gtaaaggcgg cccagaaagc tgcagccttc gaagaccagg agaatgagac ggtggtggtg
     8641 aaggagaaaa tggttggggg cattgcccag attatcgcag cacaggaaga gatgcttcgg
     8701 aaggaacgag agctggaaga ggctcggaaa aagctcgccc agatccggca gcagcagtac
     8761 aagttcttgc cttcagagct tcgagacgag cactaatgag aattcgcggc cgctcgagtc
     8821 tagagggccc gcggttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg
     8881 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     8941 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9001 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9061 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     9121 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     9181 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     9241 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     9301 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     9361 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     9421 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9481 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9541 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9601 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     9661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9721 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9781 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9901 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9961 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
    10021 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
    10081 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
    10141 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
    10201 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
    10261 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
    10321 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
    10381 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
    10441 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
    10501 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    10561 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    10621 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10681 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10741 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    10801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    11821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12901 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    12961 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    13021 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
    13081 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
    13141 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
    13201 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
    13261 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
    13321 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
    13381 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
    13441 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
    13501 tctacgtatt agtcatcgct attaccatg
//
