LOCUS       sequence-325549-       13544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NEPTUNE tagged FL human talin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard
            T
  TITLE     A distinct talin2 structure directs isoform specificity in cell 
            adhesion.
  JOURNAL   J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 
            10.1074/jbc.RA119.010789. Epub 2020 Jun 30.
  PUBMED    32605925
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010789"; journalName: "J Biol Chem"; date: 
            "2020-09-11- 11"; volume: "295"; issue: "37"; pages: "12885-12899"
COMMENT     Sequence Label: pCDNA_NEPTUNE_hTLN2-FL
FEATURES             Location/Qualifiers
     source          1..13544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             771..800
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             801..1532
                     /codon_start=1
                     /product="brighter version of the far-red fluorescent 
                     protein mNeptune2 with slightly blue-shifted spectra"
                     /label=mNeptune2.5
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             1539..1562
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9233..9262
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9278..9295
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9318..9335)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9324..9548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9594..10022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9681..9700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9891..9912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10031..10051)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10036..10365
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10216..10351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10278..10297
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10413..10460
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10479..10853
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAGWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10983..11104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11020..11039)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11074..11093
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11153..11169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11153..11169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11166..11188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11177..11193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11201..11207,11208..11225,11226..11231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11246..11267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11384..11401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11555..12143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11635..11654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12314..13105,13106..13174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12937..12956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13175..13279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13354..13373)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg ctagcggtac cgccaccatg catcaccacc
      781 accatcatca tcaccaccat atggtgagca agggcgagga gctgatcaag gagaacatgc
      841 acacgaagct gtacatggaa ggcaccgtga acaaccacca cttcaagtgc acccacgaag
      901 gggagggcaa gccctacgag ggcacccaga ccaacaggat taaggtggtg gagggaggcc
      961 ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc aagaccttca
     1021 tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag ggcttcacat
     1081 gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag gacaccagcc
     1141 tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc ccatccaacg
     1201 gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc ctgtaccccg
     1261 ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc gggggccacc
     1321 tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
     1381 ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac aatgagacct
     1441 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     1501 acaaacttaa tggcatggac gagctgtaca agggatccct ggaagttctg ttccaggggc
     1561 cccatatggt ggccctgtcc tttaagattt gtgtgcgcca ctgcaacgtg gtgaagacca
     1621 tgcagtttga accatctaca gctgtgtacg atgcgtgtcg agtcattcgg gaacgggtgc
     1681 ctgaggcaca agctgggcaa gcttctgact atggactctt tctttcggat gaagacccga
     1741 ggaaagggat ttggctggaa gcgggcagaa cactggatta ctacatgttg cggaatgggg
     1801 atattttgga atataaaaag aaacagagac ctcagaaaat ccggatgctg gatggatctg
     1861 tgaagacagt gatggtggat gattccaaga ctgtggggga gctcctggtc actatttgta
     1921 gcagaatagg aataacaaat tatgaagaat actccttaat ccaagaaact atcgaagaaa
     1981 agaaagagga aggaacgggc acactcaaaa aagacaggac actgttacga gatgagagga
     2041 aaatggagaa gttgaaggcc aagctgcaca cagatgatga cctaaattgg ctggatcaca
     2101 gccgaacatt cagagaacaa ggagtagatg aaaacgaaac gttgctgctt agacggaagt
     2161 tcttttactc tgatcagaat gtagattcga gagaccccgt gcagctgaac ttgctttatg
     2221 ttcaggcaca ggatgacatc ctgaatggct ctcaccctgt ctccttcgag aaagcttgcg
     2281 agtttggtgg atttcaagcc cagatacaat ttggacctca tgtggaacat aaacacaaac
     2341 ctggattttt agatctgaag gaattcctgc ccaaagaata catcaagcag agaggagctg
     2401 aaaagaggat ctttcaggag cataagaact gcggagagat gagcgagata gaagccaagg
     2461 tcaagtacgt caaactcgca cggtccctcc gcacatatgg cgtgtccttc ttcctggtga
     2521 aggagaagat gaaaggcaag aacaagctgg tgcctcgcct gctggggatc accaaagact
     2581 cggtgatgcg cgtggatgag aagaccaagg aagtgctgca ggagtggccc ctcaccaccg
     2641 tcaagcgctg ggcagcctca cccaagagct tcacactgga ttttggggag tatcaggaaa
     2701 gctactattc agtacaaacc accgagggag agcagatatc ccagctgatt gcaggctaca
     2761 ttgacatcat cctgaaaaag aaacaaagta aagatcgatt tggactagaa ggtgatgagg
     2821 agtcaaccat gttagaagag tccgtttccc caaaaaagtc caccatcttg cagcagcagt
     2881 tcaaccggac cgggaaggca gagcacggct cagtggcgct gccggccgtg atgcgctcgg
     2941 gctccagcgg gcctgagacc ttcaacgttg gcagcatgcc ctcgccacag cagcaggtca
     3001 tggttgggca gatgcaccga ggccacatgc cgccactgac ctcagcccag caggccctga
     3061 tggggaccat caacacaagc atgcacgccg tccagcaggc ccaggatgat ctcagtgagc
     3121 tcgactcgct gccacctctc ggccaggata tggcatctag ggtatgggtt cagaacaaag
     3181 tcgacgaatc caaacacgga atccattctc aagttgatgc tatcacggcc ggaacggctt
     3241 cagttgttaa cctcacagct ggtgaccctg cagacactga ctacacagct gtgggatgtg
     3301 cgatcaccac tatttcttcc aacccgacgg agatgtccaa gggtgtgaag ctattggccg
     3361 ccctcatgga tgatgaggtg ggcagcgggg aggacttgct cagagctgcc aggaccctcg
     3421 ctggggcggt gtcagacttg ctgaaagctg tgcagcctac ttctggagag cctcgacaga
     3481 cagttttgac tgctgctggc agcatcggac aagccagtgg ggatcttctg agacagattg
     3541 gagagaatga gactgatgag cgattccagg atgttttaat gagtttggcc aaagctgttg
     3601 ccaatgcagc tgccatgttg gtactaaagg caaagaatgt tgcccaagtg gccgaagaca
     3661 ctgtcctaca gaacagggta attgctgctg ccacccagtg tgccctctcc acctcccagc
     3721 ttgtggcatg tgccaaggtt gtgagcccca ctattagctc ccctgtgtgc caggagcagc
     3781 tgattgaagc agggaagctg gtggaccgct cggtggagaa ctgtgtccgt gcctgccagg
     3841 cggccactac cgatagtgag ctcctgaagc aggtcagcgc agcggccagc gtggtcagcc
     3901 aggccctcca tgatctcctg cagcatgtgc ggcagtttgc cagccgaggc gagcccatcg
     3961 gccgctacga ccaggctact gacaccatca tgtgtgtcac cgagagcatc ttcagctcca
     4021 tgggtgacgc tggtgaaatg gtgcgccagg cgcgggttct ggcccaagcc acatcagacc
     4081 tcgtcaatgc catgaggtca gatgcagaag ccgaaatcga catggagaat tcaaagaagc
     4141 tcctggcagc agcaaaactc ttagctgact ccactgctcg catggtggaa gctgcagagg
     4201 gggctgcagc caacccagag aatgaggacc agcagcaaag gctgagagaa gctgcagaag
     4261 gcctccgggt agcaaccaac gcagctgccc agaatgctat taagaaaaaa attgtcaacc
     4321 gactggaggt tgcagccaag caggccgcag cggcagctac acagaccatc gccgcctccc
     4381 agaatgcagc tgtttccaac aagaaccctg cggcccagca gcagctggtc cagagttgca
     4441 aggcagtggc tgatcacatc cctcagctgg tccagggagt gagggggagc caagctcaag
     4501 ctgaagacct gagtgcccag ctggctctca tcatctccag ccagaacttc ctccagcctg
     4561 gaagcaagat ggtgtcctct gccaaagccg cagtgcccac cgtgagtgac caggccgcag
     4621 ccatgcagct gagccagtgt gccaagaacc tggccaccag cttggcggag ctgcgtaccg
     4681 cctcgcagaa ggcccatgaa gcttgtggtc cgatggaaat cgattcagct ctgaatacgg
     4741 tgcagacgct taagaatgaa ctgcaggatg ccaagatggc agtcgtggag agccagctga
     4801 agccacttcc aggggaaacg ctggaaaaat gtgctcagga cctgggaagc acatccaagg
     4861 cggtgggctc ctccatggca cagctgctga cctgtgctgc tcaaggcaac gaacactaca
     4921 caggggtggc tgctagagag acggcccaag ctctgaaaac actggcccag gccgcccgtg
     4981 gagtggctgc atcgacaacc gaccccgcgg ccgcccatgc catgttagat tctgctcgag
     5041 acgtgatgga gggctccgcc atgctcattc aagaggccaa gcaggccctg attgcacctg
     5101 gagatgcaga gcgtcaacaa agactggctc aggtggctaa agccgtctca cactccttga
     5161 ataactgcgt aaattgcctc cctgggcaga aggatgtgga cgtggccttg aagagcatcg
     5221 gggagtccag caagaagctg cttgtggatt cgctacctcc aagcacgaag cctttccagg
     5281 aagcccagag tgaactgaac caggcagcag ctgatctgaa ccagtctgct ggggaagtgg
     5341 tccatgccac ccggggccag agtggagagt tggctgcagc ctctggaaag ttcagtgatg
     5401 attttgatga attcctcgat gctggcattg agatggctgg ccaagctcag acaaaagaag
     5461 accagatcca agtgataggg aacctcaaga atatctcgat ggcatccagc aagctgctgt
     5521 tagctgccaa gtctctctct gtagatccag gagctcccaa tgcgaaaaat ctcctggctg
     5581 cagctgcaag agctgtgaca gagagcatca atcaactcat cactctgtgt acccaacaag
     5641 ctccgggcca gaaagagtgc gataatgccc tgcgggagct cgagactgtg aaggggatgt
     5701 tggacaatcc taatgaacct gttagtgacc tctcttactt tgactgcatt gagagtgtga
     5761 tggaaaactc caaggttctg ggtgaatcga tggcagggat tccacagaat gccaagaccg
     5821 gagacctccc tgcctttggg gaatgtgtgg ggattgcatc caaggctctc tgtgggctga
     5881 cagaggctgc agcccaggct gcatacttgg ttggcatctc tgatccaaac agccaggcag
     5941 gccaccaggg cctggtggac cccatccagt ttgccagggc taaccaggcc atccagatgg
     6001 catgccagaa cttggtggac cctggcagca gcccatcaca ggtcctgtca gccgccacaa
     6061 ttgttgccaa gcacacgtca gccttgtgta atgcctgccg catcgcctca tccaagacgg
     6121 ccaacccagt agccaagagg cacttcgtcc agtcagccaa ggaagtcgcc aacagcactg
     6181 ccaacctggt gaagaccatc aaggccctgg atggggattt ctctgaagac aaccgcaata
     6241 agtgtcgcat cgccaccgca cccttgattg aagctgtgga gaacctgaca gcgttcgcct
     6301 caaaccctga gtttgtcagc attcctgccc agatcagctc cgagggttcc caggcacagg
     6361 aaccaatcct ggtctcagcc aagaccatgc tggagagttc atcgtacctc attcgcactg
     6421 cgcgctctct ggccatcaac cccaaagacc cacccacctg gtctgtactg gctggacatt
     6481 cccatacagt gtccgactcc atcaagagtc tcatcacttc tatcagggac aaggcccctg
     6541 gacagaggga gtgtgattac tccatcgatg gcatcaaccg gtgcatccgg gacatcgagc
     6601 aggcctcgct ggccgccgtc agccagagcc tggccacgag ggacgacatc tctgtggagg
     6661 ccctgcagga gcagctgact tcggtggtcc aggaaatcgg acaccttatc gatcccatcg
     6721 ccacagcggc tcggggagaa gcagctcagc tgggacataa ggtgacacaa ctggcaagct
     6781 attttgagcc cttgatctta gccgcagttg gtgtggcctc caagattctt gatcatcagc
     6841 agcagatgac ggtgctggac cagaccaaga ctctcgcaga gtctgccttg cagatgttgt
     6901 atgcagccaa agaaggtggc ggaaacccca aggcacaaca cacccatgac gccatcacag
     6961 aggccgccca gttgatgaag gaagccgtgg atgacatcat ggtgacgctg aacgaagctg
     7021 ccagtgaagt ggggctggtt gggggcatgg tggacgccat tgcagaagcc atgagcaagc
     7081 tggatgaagg cactcctcca gaaccaaagg gaacatttgt cgactatcag acgactgtgg
     7141 ttaaatactc caaagccatt gcggtgacag ctcaggaaat gatgactaag tcggttacta
     7201 acccggagga gttgggagga ctggcttcac aaatgaccag tgactatggg cacctggctt
     7261 tccagggcca gatggcagca gccacggcgg aaccagagga gatcggattc cagattcgca
     7321 ctcgtgtgca ggacctgggc cacggctgta tcttcctggt gcagaaggca ggggccctcc
     7381 aggtctgccc cacagacagc tacaccaaga gggagctgat cgaatgcgcc cgtgccgtca
     7441 cggaaaaggt ctccttggtg ctctcggctc tccaggccgg gaacaaagga acccaggcat
     7501 gcattacagc cgccaccgct gtgtctggga tcattgccga cctggacacc accattatgt
     7561 ttgcaacagc ggggacgctg aatgcagaga acagtgagac cttcgcagac cacagggaga
     7621 acattctcaa gacggccaag gccttggtag aagacacgaa actacttgtg tcaggagctg
     7681 cgtccactcc tgacaagctg gcccaggcgg cccagtcctc agcagccacc atcacccagc
     7741 tcgcagaagt ggtcaagctg ggggcagcca gcctgggctc cgacgacccc gagacccagg
     7801 tggttttgat caatgccatc aaagatgtgg ccaaggccct ttctgatctc atcagtgcta
     7861 ccaagggagc tgccagcaag ccagtggacg acccttccat gtaccagctc aagggggctg
     7921 ccaaggtgat ggtgaccaat gtcacctcgc tcctcaagac tgtaaaggca gtggaggatg
     7981 aggccacccg gggcaccagg gcgcttgagg ccacaattga atgcataaag caggagctta
     8041 cggtgttcca gtcaaaagac gtacctgaaa agacatcatc acctgaagaa tccataagga
     8101 tgacgaaagg catcaccatg gcaacagcca aagccgtggc agctgggaac tcatgtagac
     8161 aggaggacgt gattgctact gccaacctga gccggaaagc cgtgtcagat atgttgacgg
     8221 cttgcaagca agcatccttc caccccgatg tcagtgacga ggtgagaacc agagccttgc
     8281 gtttcgggac ggagtgcacc cttggctact tggacctcct ggagcacgtc ttggtgattc
     8341 ttcagaaacc aaccccagaa ctcaagcagc agctggccgc tttctccaag cgagtcgccg
     8401 gcgctgtgac agagctcatc caggcggcgg aagccatgaa aggaacagag tgggtggatc
     8461 cagaagaccc aactgtcatt gcagaaacag agttactggg ggctgcagca tccatcgaag
     8521 ctgctgctaa gaagttagag caactgaagc caagagcaaa accaaaacaa gcggatgaga
     8581 ccctggactt tgaggaacag atcttggaag ctgctaaatc cattgctgct gccacaagcg
     8641 ccctggtcaa atcggcctca gcagcccaga gggagctggt ggcccaagga aaggtgggct
     8701 ccatccctgc caacgctgca gacgacggac agtggtcaca agggctgatt tctgctgccc
     8761 ggatggtggc ggctgcgacc agcagtctct gtgaggcggc caatgcctcc gttcagggac
     8821 acgccagcga ggagaagctc atctcatctg ccaagcaggt cgccgcttcc acggctcagc
     8881 tgctggtggc ctgcaaggtg aaggccgacc aggattcaga ggccatgagg cggctacagg
     8941 cggcaggaaa tgctgtgaaa agagcctcag acaatcttgt ccgtgcagcc cagaaggcag
     9001 catttggcaa agctgatgac gacgatgttg tagtgaaaac caagtttgtg gggggcattg
     9061 ctcagatcat cgccgcccag gaagaaatgc taaagaaaga gcgagaactg gaagaagcaa
     9121 ggagaaaact ggcccaaatc cgccagcagc agtataagtt tttacccacc gagctgaggg
     9181 aagatgaggg ctgagaattc gcggccgctc gagtctagag ggcccgcggt tcgaacaaaa
     9241 actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc accattgagt
     9301 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     9361 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     9421 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     9481 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     9541 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     9601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9781 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     9841 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     9901 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     9961 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    10021 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    10081 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtatggaaa gtccccaggc
    10141 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    10201 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10261 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
    10321 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
    10381 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
    10441 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
    10501 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
    10561 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
    10621 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
    10681 tgcgcggcct ggacgagctg tacgccgggt ggtcggaggt cgtgtccacg aacttccggg
    10741 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
    10801 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
    10861 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    10921 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    10981 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    11041 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11101 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    11161 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11221 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    11281 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    11341 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    11401 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11461 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    11521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    11581 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    11641 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    11701 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    11761 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    11821 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    11881 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11941 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    12001 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    12061 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    12121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    12181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    12241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    12301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    12361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    12421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    12481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    12541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    12601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    12661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    12721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    12781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    12841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    12901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    12961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    13021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    13081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    13141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    13201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    13261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    13321 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    13381 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    13441 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    13501 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
