LOCUS       sequence-325550-       13529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged FL human talin2 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard
            T
  TITLE     A distinct talin2 structure directs isoform specificity in cell 
            adhesion.
  JOURNAL   J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 
            10.1074/jbc.RA119.010789. Epub 2020 Jun 30.
  PUBMED    32605925
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010789"; journalName: "J Biol Chem"; date: 
            "2020-09-11- 11"; volume: "295"; issue: "37"; pages: "12885-12899"
COMMENT     Sequence Label: pCDNA_GFP_hTLN2-FL-LBD
FEATURES             Location/Qualifiers
     source          1..13529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             771..800
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(801..803,804..806,807..1517)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(801..803,804..806,807..1517)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(846..867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1107..1126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1524..1547
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9218..9247
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9263..9280
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9303..9320)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9309..9533
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9579..10007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9666..9685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9876..9897
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10016..10036)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10021..10350
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10201..10336
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10263..10282
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10398..10445
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10464..10838
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAGWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10968..11089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11005..11024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11059..11078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11138..11154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11138..11154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11151..11173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11162..11178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11186..11192,11193..11210,11211..11216))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11231..11252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11369..11386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11540..12128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11620..11639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12299..13090,13091..13159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12922..12941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13160..13264)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13339..13358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg ctagcggtac cgccaccatg catcaccacc
      781 accatcatca tcaccaccat atggtgagca agggcgagga gctgttcacc ggggtggtgc
      841 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      901 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      961 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1021 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1081 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1141 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1201 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1261 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1321 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc accctcggca
     1501 tggacgagct gtacaaggga tccctggaag ttctgttcca ggggccccat atggtggccc
     1561 tgtccttaaa gatttgtgtg cgccactgca acgtggtgaa gaccatgcag tttgaaccat
     1621 ctacagctgt gtacgatgcg tgtcgagtca ttcgggaacg ggtgcctgag gcacaagctg
     1681 ggcaagcttc tgactatgga ctctttcttt cggatgaaga cccgaggaaa gggatttggc
     1741 tggaagcggg cagaacactg gattactaca tgttgcggaa tggggatatt ttggaatata
     1801 aaaagaaaca gagacctcag aaaatccgga tgctggatgg atctgtgaag acagtgatgg
     1861 tggatgattc caagactgtg ggggagctcc tggtcactat ttgtagcaga ataggaataa
     1921 caaattatga agaatactcc ttaatccaag aaactatcga agaaaagaaa gaggaaggaa
     1981 cgggcacact caaaaaagac aggacactgt tacgagatga gaggaaaatg gagaagttga
     2041 aggccaagct gcacacagat gatgacctaa attggctgga tcacagccga acattcagag
     2101 aacaaggagt agatgaaaac gaaacgttgc tgcttagacg gaagttcttt tactctgatc
     2161 agaatgtaga ttcgagagac cccgtgcagc tgaacttgct ttatgttcag gcacaggatg
     2221 acatcctgaa tggctctcac cctgtctcct tcgagaaagc ttgcgagttt ggtggatttc
     2281 aagcccagat acaatttgga cctcatgtgg aacataaaca caaacctgga tttttagatc
     2341 tgaaggaatt cctgcccaaa gaatacatcc agcagagagg agctgaaaag aggatctttc
     2401 aggagcataa gaactgcgga gagatgagcg agatagaagc caaggtcaag tacgtcaaac
     2461 tcgcacggtc cctccgcaca tatggcgtgt ccttcttcct ggtgcaggag aagatgaaag
     2521 gcaagaacca gctggtgcct cgcctgctgg ggatcaccaa agactcggtg atgcgcgtgg
     2581 atcagcagac caaggaagtg ctgcaggagt ggcccctcac caccgtcaag cgctgggcag
     2641 cctcacccaa gagcttcaca ctggattttg gggagtatca ggaaagctac tattcagtac
     2701 aaaccaccga gggagagcag atatcccagc tgattgcagg ctacattgac atcatcctga
     2761 aaaagaaaca aagtaaagat cgatttggac tagaaggtga tgaggagtca accatgttag
     2821 aagagtccgt ttccccaaaa aagtccacca tcttgcagca gcagttcaac cggaccggga
     2881 aggcagagca cggctcagtg gcgctgccgg ccgtgatgcg ctcgggctcc agcgggcctg
     2941 agaccttcaa cgttggcagc atgccctcgc cacagcagca ggtcatggtt gggcagatgc
     3001 accgaggcca catgccgcca ctgacctcag cccagcaggc cctgatgggg accatcaaca
     3061 caagcatgca cgccgtccag caggcccagg atgatctcag tgagctcgac tcgctgccac
     3121 ctctcggcca ggatatggca tctagggtat gggttcagaa caaagtcgac gaatccaaac
     3181 acggaatcca ttctcaagtt gatgctatca cggccggaac ggcttcagtt gttaacctca
     3241 cagctggtga ccctgcagac actgactaca cagctgtggg atgtgcgatc accactattt
     3301 cttccaaccc gacggagatg tccaagggtg tgaagctatt ggccgccctc atggatgatg
     3361 aggtgggcag cggggaggac ttgctcagag ctgccaggac cctcgctggg gcggtgtcag
     3421 acttgttgaa agctgtgcag cctacttctg gagagcctcg acagacagtt ttgactgctg
     3481 ctggcagcat cggacaagcc agtggggatc ttctgagaca gattggagag aatgagactg
     3541 atgagcgatt ccaggatgtt ttaatgagtt tggccaaagc tgttgccaat gcagctgcca
     3601 tgttggtact aaaggcaaag aatgttgccc aagtggccga agacactgtc ctacagaaca
     3661 gggtaattgc tgctgccacc cagtgtgccc tctccacctc ccagcttgtg gcatgtgcca
     3721 aggttgtgag ccccactatt agctcccctg tgtgccagga gcagctgatt gaagcaggga
     3781 agctggtgga ccgctcggtg gagaactgtg tccgtgcctg ccaggcggcc actaccgata
     3841 gtgagctcct gaagcaggtc agcgcagcgg ccagcgtggt cagccaggcc ctccatgatc
     3901 tcctgcagca tgtgcggcag tttgccagcc gaggcgagcc catcggccgc tacgaccagg
     3961 ctactgacac catcatgtgt gtcaccgaga gcatcttcag ctccatgggt gacgctggtg
     4021 aaatggtgcg ccaggcgcgg gttctggccc aagccacatc agacctcgtc aatgccatga
     4081 ggtcagatgc agaagccgaa atcgacatgg agaattcaaa gaagctcctg gcagcagcaa
     4141 aactcttagc tgactccact gctcgcatgg tggaagctgc agagggggct gcagccaacc
     4201 cagagaatga ggaccagcag caaaggctga gagaagctgc agaaggcctc cgggtagcaa
     4261 ccaacgcagc tgcccagaat gctattaaga aaaaaattgt caaccgactg gaggttgcag
     4321 ccaagcaggc cgcagcggca gctacacaga ccatcgccgc ctcccagaat gcagctgttt
     4381 ccaacaagaa ccctgcggcc cagcagcagc tggtccagag ttgcaaggca gtggctgatc
     4441 acatccctca gctggtccag ggagtgaggg ggagccaagc tcaagctgaa gacctgagtg
     4501 cccagctggc tctcatcatc tccagccaga acttcctcca gcctggaagc aagatggtgt
     4561 cctctgccaa agccgcagtg cccaccgtga gtgaccaggc cgcagccatg cagctgagcc
     4621 agtgtgccaa gaacctggcc accagcttgg cggagctgcg taccgcctcg cagaaggccc
     4681 atgaagcttg tggtccgatg gaaatcgatt cagctctgaa tacggtgcag acgcttaaga
     4741 atgaactgca ggatgccaag atggcagccg tggagagcca gctgaagcca cttccagggg
     4801 aaacgctgga aaaatgtgct caggacctgg gaagcacatc caaggcggtg ggctcctcca
     4861 tggcacagct gctgacctgt gctgctcaag gcaacgaaca ctacacaggg gtggctgcta
     4921 gagagacggc ccaagctctg aaaacactgg cccaggccgc ccgtggagtg gctgcatcga
     4981 caaccgaccc cgcggccgcc catgccatgt tagattctgc tcgagacgtg atggagggct
     5041 ccgccatgct cattcaagag gccaagcagg ccctgattgc acctggagat gcagagcgtc
     5101 aacaaagact ggctcaggtg gctaaagccg tctcacactc cttgaataac tgcgtaaatt
     5161 gcctccctgg gcagaaggat gtggacgtgg ccttgaagag catcggggag tccagcaaga
     5221 agctgcttgt ggattcgcta cctccaagca cgaagccttt ccaggaagcc cagagtgaac
     5281 tgaaccaggc agcagctgat ctgaaccagt ctgctgggga agtggtccat gccacccggg
     5341 gccagagtgg agagttggct gcagcctctg gaaagttcag tgatgatttt gatgaattcc
     5401 tcgatgctgg cattgagatg gctggccaag ctcagacaaa agaagaccag atccaagtga
     5461 tagggaacct caagaatatc tcgatggcat ccagcaagct gctgttagct gccaagtctc
     5521 tctctgtaga tccaggagct cccaatgcga aaaatctcct ggctgcagct gcaagagctg
     5581 tgacagagag catcaatcaa ctcatcactc tgtgtaccca acaagctccg ggccagaaag
     5641 agtgcgataa tgccctgcgg gagctcgaga ctgtgaaggg gatgttggac aatcctaatg
     5701 aacctgttag tgacctctct tactttgact gcattgagag tgtgatggaa aactccaagg
     5761 ttctgggtga atcgatggca gggattccac agaatgccaa gaccggagac ctccctgcct
     5821 ttggggaatg tgtggggatt gcatccaagg ctctctgtgg gctgacagag gctgcagccc
     5881 aggctgcata cttggttggc atctctgatc caaacagcca ggcaggccac cagggcctgg
     5941 tggaccccat ccagtttgcc agggctaacc aggccatcca gatggcatgc cagaacttgg
     6001 tggaccctgg cagcagccca tcacaggtcc tgtcagccgc cacaattgtt gccaagcaca
     6061 cgtcagcctt gtgtaatgcc tgccgcatcg cctcatccaa gacggccaac ccagtagcca
     6121 agaggcactt cgtccagtca gccaaggaag tcgccaacag cactgccaac ctggtgaaga
     6181 ccatcaaggc cctggatggg gatttctctg aagacaaccg caataagtgt cgcatcgcca
     6241 ccgcaccctt gattgaagct gtggagaacc tgacagcgtt cgcctcaaac cctgagtttg
     6301 tcagcattcc tgcccagatc agctccgagg gttcccaggc acaggaacca atcctggtct
     6361 cagccaagac catgctggag agttcatcgt acctcattcg cactgcgcgc tctctggcca
     6421 tcaaccccaa agacccaccc acctggtctg tactggctgg acattcccat acagtgtccg
     6481 actccatcaa gagtctcatc acttctatca gggacaaggc ccctggacag agggagtgtg
     6541 attactccat cgatggcatc aaccggtgca tccgggacat cgagcaggcc tcgctggccg
     6601 ccgtcagcca gagcctggcc acgagggacg acatctctgt ggaggccctg caggagcagc
     6661 tgacttcggt ggtccaggaa atcggacacc ttatcgatcc catcgccaca gcggctcggg
     6721 gagaagcagc tcagctggga cataaggtga cacaactggc aagctatttt gagcccttga
     6781 tcttagccgc agttggtgtg gcctccaaga ttcttgatca tcagcagcag atgacggtgc
     6841 tggaccagac caagactctc gcagagtctg ccttgcagat gttgtatgca gccaaagaag
     6901 gtggcggaaa ccccaaggca caacacaccc atgacgccat cacagaggcc gcccagttga
     6961 tgaaggaagc cgtggatgac atcatggtga cgctgaacga agctgccagt gaagtggggc
     7021 tggttggggg catggtggac gccattgcag aagccatgag caagctggat gaaggcactc
     7081 ctccagaacc aaagggaaca tttgtcgact atcagacgac tgtggttaaa tactccaaag
     7141 ccattgcggt gacagctcag gaaatgatga ctaagtcggt tactaacccg gaggagttgg
     7201 gaggactggc ttcacaaatg accagtgact atgggcacct ggctttccag ggccagatgg
     7261 cagcagccac ggcggaacca gaggagatcg gattccagat tcgcactcgt gtgcaggacc
     7321 tgggccacgg ctgtatcttc ctggtgcaga aggcaggggc cctccaggtc tgccccacag
     7381 acagctacac caagagggag ctgatcgaat gcgcccgtgc cgtcacggaa aaggtctcct
     7441 tggtgctctc ggctctccag gccgggaaca aaggaaccca ggcatgcatt acagccgcca
     7501 ccgctgtgtc tgggatcatt gccgacctgg acaccaccat tatgtttgca acagcgggga
     7561 cgctgaatgc agagaacagt gagaccttcg cagaccacag ggagaacatt ctcaagacgg
     7621 ccaaggcctt ggtagaagac acgaaactac ttgtgtcagg agctgcgtcc actcctgaca
     7681 agctggccca ggcggcccag tcctcagcag ccaccatcac ccagctcgca gaagtggtca
     7741 agctgggggc agccagcctg ggctccgacg accccgagac ccaggtggtt ttgatcaatg
     7801 ccatcaaaga tgtggccaag gccctttctg atctcatcag tgctaccaag ggagctgcca
     7861 gcaagccagt ggacgaccct tccatgtacc agctcaaggg ggctgccaag gtgatggtga
     7921 ccaatgtcac ctcgctcctc aagactgtaa aggcagtgga ggatgaggcc acccggggca
     7981 ccagggcgct tgaggccaca attgaatgca taaagcagga gcttacggtg ttccagtcaa
     8041 aagacgtacc tgaaaagaca tcatcacctg aagaatccat aaggatgacg aaaggcatca
     8101 ccatggcaac agccaaagcc gtggcagctg ggaactcatg tagacaggag gacgtgattg
     8161 ctactgccaa cctgagccgg aaagccgtgt cagatatgtt gacggcttgc aagcaagcat
     8221 ccttccaccc cgatgtcagt gacgaggtga gaaccagagc cttgcgtttc gggacggagt
     8281 gcacccttgg ctacttggac ctcctggagc acgtcttggt gattcttcag aaaccaaccc
     8341 cagaactcaa gcagcagctg gccgctttct ccaagcgagt cgccggcgct gtgacagagc
     8401 tcatccaggc ggcggaagcc atgaaaggaa cagagtgggt ggatccagaa gacccaactg
     8461 tcattgcaga aacagagtta ctgggggctg cagcatccat cgaagctgct gctaagaagt
     8521 tagagcaact gaagccaaga gcaaaaccaa aacaagcgga tgagaccctg gactttgagg
     8581 aacagatctt ggaagctgct aaatccattg ctgctgccac aagcgccctg gtcaaatcgg
     8641 cctcagcagc ccagagggag ctggtggccc aaggaaaggt gggctccatc cctgccaacg
     8701 ctgcagacga cggacagtgg tcacaagggc tgatttctgc tgcccggatg gtggcggctg
     8761 cgaccagcag tctctgtgag gcggccaatg cctccgttca gggacacgcc agcgaggaga
     8821 agctcatctc atctgccaag caggtcgccg cttccacggc tcagctgctg gtggcctgca
     8881 aggtgaaggc cgaccaggat tcagaggcca tgaggcggct acaggcggca ggaaatgctg
     8941 tgaaaagagc ctcagacaat cttgtccgtg cagcccagaa ggcagcattt ggcaaagctg
     9001 atgacgacga tgttgtagtg aaaaccaagt ttgtgggggg cattgctcag atcatcgccg
     9061 cccaggaaga aatgctaaag aaagagcgag aactggaaga agcaaggaga aaactggccc
     9121 aaatccgcca gcagcagtat aagtttttac ccaccgagct gagggaagat gagggctgag
     9181 aattcgcggc cgctcgagtc tagagggccc gcggttcgaa caaaaactca tctcagaaga
     9241 ggatctgaat atgcataccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     9301 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9361 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9421 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9481 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     9541 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     9601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
    10021 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10081 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10141 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    10201 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10261 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
    10321 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
    10381 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
    10441 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
    10501 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
    10561 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
    10621 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
    10681 agctgtacgc cgggtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
    10741 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
    10801 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    10861 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    10921 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    10981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    11041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    11101 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    11161 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    11221 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    11281 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11341 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    11401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    11461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    11521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    11581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11941 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    12001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    12121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    12181 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    12241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    12301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    12361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    12421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    12481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    12541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    12601 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12841 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12901 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    13021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    13081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    13141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    13201 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    13261 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    13321 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    13381 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    13441 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    13501 tcgcgatgta cgggccagat atacgcgtt
//
