LOCUS       sequence-325551-        9008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Ren Q, Sretenovic S, Liu S, Tang X, Huang L, He Y, Liu L, Guo Y, 
            Zhong Z, Liu G, Cheng Y, Zheng X, Pan C, Yin D, Zhang Y, Li W, Qi L,
            Li C, Qi Y, Zhang Y
  TITLE     PAM-less plant genome editing using a CRISPR-SpRY toolbox.
  JOURNAL   Nat Plants. 2021 Jan 4. pii: 10.1038/s41477-020-00827-4. doi: 
            10.1038/s41477-020-00827-4.
  PUBMED    33398158
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2021 Jan 4. pii: 10.1038/s41477-020-00827-4. doi: 
            10.1038/s41477-020-00827-4."
COMMENT     Sequence Label: pYPQ262m
FEATURES             Location/Qualifiers
     source          1..9008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             140..160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1355..1420
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1427..1447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1472..1495,1496..1498,1499..5572)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5485..5493
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5573..5620
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    6295..6418
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6435..6454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6436..6454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6459..6475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6459..6475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6906..7697
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7790..8378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8279..8298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8532..8549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8708..8735)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(8827..8913)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8962..8984
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8976..8993
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8977..8993
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgatgaaac ggacagccga
      121 cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc tctgaagtcg agtttagcca
      181 cgagtattgg atgaggcacg cactgaccct ggcaaagcga gcatgggatg aaagagaagt
      241 ccccgtgggc gccgtgctgg tgcacaacaa tagagtgatc ggagagggat ggaacaggcc
      301 aatcggccgc cacgacccta ccgcacacgc agagatcatg gcactgaggc agggaggcct
      361 ggtcatgcag aattaccgcc tgatcgatgc caccctgtat gtgacactgg agccatgcgt
      421 gatgtgcgca ggagcaatga tccacagcag gatcggaaga gtggtgttcg gagcacggga
      481 cgccaagacc ggcgcagcag gctccctgat ggatgtgctg caccaccccg gcatgaacca
      541 ccgggtggag atcacagagg gaatcctggc agacgagtgc gccgccctgc tgagcgattt
      601 ctttagaatg cggagacagg agatcaaggc ccagaagaag gcacagagct ccaccgactc
      661 tggaggatct agcggaggat cctctggaag cgagacacca ggcacaagcg agtccgccac
      721 accagagagc tccggcggct cctccggagg atcctctgag gtggagtttt cccacgagta
      781 ctggatgaga catgccctga ccctggccaa gagggcacgc gatgagaggg aggtgcctgt
      841 gggagccgtg ctggtgctga acaatagagt gatcggcgag ggctggaaca gagccatcgg
      901 cctgcacgac ccaacagccc atgccgaaat tatggccctg agacagggcg gcctggtcat
      961 gcagaactac agactgattg acgccaccct gtacgtgaca ttcgagcctt gcgtgatgtg
     1021 cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg tttggcgtga ggaacgcaaa
     1081 aaccggcgcc gcaggctccc tgatggacgt gctgcactac cccggcatga atcaccgcgt
     1141 cgaaattacc gagggaatcc tggcagatga atgtgccgcc ctgctgtgct atttctttcg
     1201 gatgcctaga caggtgttca atgctcagaa gaaggcccag agctccaccg actccggagg
     1261 atctagcgga ggctcctctg gctctgagac acctggcaca agcgagagcg caacacctga
     1321 aagcagcggg ggcagcagcg gggggtcaac catggattac aaggaccacg acggggatta
     1381 caaggaccac gacattgatt acaaggatga tgatgacaag atggctccga agaagaagag
     1441 gaaggttggc atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgctat
     1501 tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa
     1561 gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct
     1621 cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg
     1681 gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc
     1741 gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa
     1801 gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa
     1861 gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct
     1921 ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga
     1981 gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac
     2041 gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat
     2101 cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg
     2161 cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa
     2221 cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata
     2281 cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct
     2341 cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga
     2401 gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga
     2461 tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt
     2521 cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga
     2581 gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt
     2641 gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc
     2701 acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc
     2761 tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta
     2821 cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga
     2881 gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt
     2941 catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca
     3001 ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac
     3061 cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct
     3121 cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa
     3181 gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct
     3241 ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga
     3301 gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat
     3361 gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct
     3421 caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg
     3481 ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg
     3541 caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc
     3601 tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc
     3661 agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat
     3721 gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca
     3781 gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct
     3841 ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct
     3901 ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa
     3961 caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc
     4021 cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc
     4081 ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct
     4141 gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct
     4201 ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt
     4261 tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg
     4321 cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca
     4381 gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc
     4441 ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg
     4501 ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa
     4561 ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac
     4621 tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga
     4681 gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca
     4741 agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct
     4801 gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta
     4861 cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa
     4921 gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg
     4981 ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa
     5041 gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa
     5101 gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa
     5161 gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga
     5221 caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga
     5281 gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct
     5341 ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca
     5401 tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat
     5461 cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc
     5521 gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc
     5581 agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag tgagctcaga gctttcgttc
     5641 gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac
     5701 cagaatccta ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     5761 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     5821 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     5881 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     5941 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     6001 agtaaaacac ttgtagttgt accattatgc ttattcacta ggcaacaaat atattttcag
     6061 acctagaaaa gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt ttagacattt
     6121 atgaactttc ctttatgtaa ttttccagaa tccttgtcag attctaatca ttgctttata
     6181 attatagtta tactcatgga tttgtagttg agtatgaaaa tattttttaa tgcattttat
     6241 gacttgccaa ttgttccgga actctagata agcttaccgg aaagggcgaa ttcgcaactt
     6301 tgtatacaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     6361 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatgcc
     6421 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     6481 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     6541 gcctcctctg gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     6601 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     6661 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6721 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     6781 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     6841 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     6901 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6961 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     7021 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     7081 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     7141 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     7201 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     7261 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     7321 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7381 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7441 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7501 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7561 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7621 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7681 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     7741 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7921 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8401 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8461 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8581 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8641 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc gagccaggaa gagtttgtag
     8701 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     8761 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     8821 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     8881 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     8941 taacgcttgc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     9001 tcgggccc
//
