LOCUS       sequence-325554-       13529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged FL human talin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard
            T
  TITLE     A distinct talin2 structure directs isoform specificity in cell 
            adhesion.
  JOURNAL   J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 
            10.1074/jbc.RA119.010789. Epub 2020 Jun 30.
  PUBMED    32605925
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010789"; journalName: "J Biol Chem"; date: 
            "2020-09-11- 11"; volume: "295"; issue: "37"; pages: "12885-12899"
COMMENT     Sequence Label: pCDNA_GFP_hTLN2-FL
FEATURES             Location/Qualifiers
     source          1..13529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..66
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        182..229
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             248..622
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAGWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    752..873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(789..808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     843..862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(922..938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(922..938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(935..957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    946..962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(970..976,977..994,995..1000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1015..1036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1153..1170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1324..1912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1404..1423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2083..2874,2875..2943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2706..2725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2944..3048)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3123..3142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3314..3693
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3694..3897
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3848..3868
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3899..3917
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3920..3938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3948..3972
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4084..4113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(4114..4116,4117..4119,4120..4830)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4114..4116,4117..4119,4120..4830)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4159..4180)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4420..4439)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4767..4788
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4837..4860
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             12531..12560
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             12576..12593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(12616..12633)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12622..12846
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12892..13320
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12979..12998)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13189..13210
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13329..13349)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(13334..13529,1..134)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(13514..13529,1..120)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
       61 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      121 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
      181 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
      241 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
      301 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
      361 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
      421 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgggtg
      481 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
      541 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
      601 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
      661 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
      721 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
      781 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      841 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
      901 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      961 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1021 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1081 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1741 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2041 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2101 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2161 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2221 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2281 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2341 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2401 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2461 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2521 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2581 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2641 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2701 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2761 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2821 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2881 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2941 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3001 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3061 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     3121 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     3181 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     3241 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     3301 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     3361 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3421 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3481 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3541 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3601 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3661 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3721 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3781 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3841 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     3901 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
     3961 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     4021 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgctagcgg taccgccacc
     4081 atgcatcacc accaccatca tcatcaccac catatggtga gcaagggcga ggagctgttc
     4141 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     4201 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     4261 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     4321 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4381 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     4441 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     4501 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     4561 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     4621 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4681 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     4741 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4801 atcaccctcg gcatggacga gctgtacaag ggatccctgg aagttctgtt ccaggggccc
     4861 catatggtgg ccctgtcctt taagatttgt gtgcgccact gcaacgtggt gaagaccatg
     4921 cagtttgaac catctacagc tgtgtacgat gcgtgtcgag tcattcggga acgggtgcct
     4981 gaggcacaag ctgggcaagc ttctgactat ggactctttc tttcggatga agacccgagg
     5041 aaagggattt ggctggaagc gggcagaaca ctggattact acatgttgcg gaatggggat
     5101 attttggaat ataaaaagaa acagagacct cagaaaatcc ggatgctgga tggatctgtg
     5161 aagacagtga tggtggatga ttccaagact gtgggggagc tcctggtcac tatttgtagc
     5221 agaataggaa taacaaatta tgaagaatac tccttaatcc aagaaactat cgaagaaaag
     5281 aaagaggaag gaacgggcac actcaaaaaa gacaggacac tgttacgaga tgagaggaaa
     5341 atggagaagt tgaaggccaa gctgcacaca gatgatgacc taaattggct ggatcacagc
     5401 cgaacattca gagaacaagg agtagatgaa aacgaaacgt tgctgcttag acggaagttc
     5461 ttttactctg atcagaatgt agattcgaga gaccccgtgc agctgaactt gctttatgtt
     5521 caggcacagg atgacatcct gaatggctct caccctgtct ccttcgagaa agcttgcgag
     5581 tttggtggat ttcaagccca gatacaattt ggacctcatg tggaacataa acacaaacct
     5641 ggatttttag atctgaagga attcctgccc aaagaataca tcaagcagag aggagctgaa
     5701 aagaggatct ttcaggagca taagaactgc ggagagatga gcgagataga agccaaggtc
     5761 aagtacgtca aactcgcacg gtccctccgc acatatggcg tgtccttctt cctggtgaag
     5821 gagaagatga aaggcaagaa caagctggtg cctcgcctgc tggggatcac caaagactcg
     5881 gtgatgcgcg tggatgagaa gaccaaggaa gtgctgcagg agtggcccct caccaccgtc
     5941 aagcgctggg cagcctcacc caagagcttc acactggatt ttggggagta tcaggaaagc
     6001 tactattcag tacaaaccac cgagggagag cagatatccc agctgattgc aggctacatt
     6061 gacatcatcc tgaaaaagaa acaaagtaaa gatcgatttg gactagaagg tgatgaggag
     6121 tcaaccatgt tagaagagtc cgtttcccca aaaaagtcca ccatcttgca gcagcagttc
     6181 aaccggaccg ggaaggcaga gcacggctca gtggcgctgc cggccgtgat gcgctcgggc
     6241 tccagcgggc ctgagacctt caacgttggc agcatgccct cgccacagca gcaggtcatg
     6301 gttgggcaga tgcaccgagg ccacatgccg ccactgacct cagcccagca ggccctgatg
     6361 gggaccatca acacaagcat gcacgccgtc cagcaggccc aggatgatct cagtgagctc
     6421 gactcgctgc cacctctcgg ccaggatatg gcatctaggg tatgggttca gaacaaagtc
     6481 gacgaatcca aacacggaat ccattctcaa gttgatgcta tcacggccgg aacggcttca
     6541 gttgttaacc tcacagctgg tgaccctgca gacactgact acacagctgt gggatgtgcg
     6601 atcaccacta tttcttccaa cccgacggag atgtccaagg gtgtgaagct attggccgcc
     6661 ctcatggatg atgaggtggg cagcggggag gacttgctca gagctgccag gaccctcgct
     6721 ggggcggtgt cagacttgct gaaagctgtg cagcctactt ctggagagcc tcgacagaca
     6781 gttttgactg ctgctggcag catcggacaa gccagtgggg atcttctgag acagattgga
     6841 gagaatgaga ctgatgagcg attccaggat gttttaatga gtttggccaa agctgttgcc
     6901 aatgcagctg ccatgttggt actaaaggca aagaatgttg cccaagtggc cgaagacact
     6961 gtcctacaga acagggtaat tgctgctgcc acccagtgtg ccctctccac ctcccagctt
     7021 gtggcatgtg ccaaggttgt gagccccact attagctccc ctgtgtgcca ggagcagctg
     7081 attgaagcag ggaagctggt ggaccgctcg gtggagaact gtgtccgtgc ctgccaggcg
     7141 gccactaccg atagtgagct cctgaagcag gtcagcgcag cggccagcgt ggtcagccag
     7201 gccctccatg atctcctgca gcatgtgcgg cagtttgcca gccgaggcga gcccatcggc
     7261 cgctacgacc aggctactga caccatcatg tgtgtcaccg agagcatctt cagctccatg
     7321 ggtgacgctg gtgaaatggt gcgccaggcg cgggttctgg cccaagccac atcagacctc
     7381 gtcaatgcca tgaggtcaga tgcagaagcc gaaatcgaca tggagaattc aaagaagctc
     7441 ctggcagcag caaaactctt agctgactcc actgctcgca tggtggaagc tgcagagggg
     7501 gctgcagcca acccagagaa tgaggaccag cagcaaaggc tgagagaagc tgcagaaggc
     7561 ctccgggtag caaccaacgc agctgcccag aatgctatta agaaaaaaat tgtcaaccga
     7621 ctggaggttg cagccaagca ggccgcagcg gcagctacac agaccatcgc cgcctcccag
     7681 aatgcagctg tttccaacaa gaaccctgcg gcccagcagc agctggtcca gagttgcaag
     7741 gcagtggctg atcacatccc tcagctggtc cagggagtga gggggagcca agctcaagct
     7801 gaagacctga gtgcccagct ggctctcatc atctccagcc agaacttcct ccagcctgga
     7861 agcaagatgg tgtcctctgc caaagccgca gtgcccaccg tgagtgacca ggccgcagcc
     7921 atgcagctga gccagtgtgc caagaacctg gccaccagct tggcggagct gcgtaccgcc
     7981 tcgcagaagg cccatgaagc ttgtggtccg atggaaatcg attcagctct gaatacggtg
     8041 cagacgctta agaatgaact gcaggatgcc aagatggcag ccgtggagag ccagctgaag
     8101 ccacttccag gggaaacgct ggaaaaatgt gctcaggacc tgggaagcac atccaaggcg
     8161 gtgggctcct ccatggcaca gctgctgacc tgtgctgctc aaggcaacga acactacaca
     8221 ggggtggctg ctagagagac ggcccaagct ctgaaaacac tggcccaggc cgcccgtgga
     8281 gtggctgcat cgacaaccga ccccgcggcc gcccatgcca tgttagattc tgctcgagac
     8341 gtgatggagg gctccgccat gctcattcaa gaggccaagc aggccctgat tgcacctgga
     8401 gatgcagagc gtcaacaaag actggctcag gtggctaaag ccgtctcaca ctccttgaat
     8461 aactgcgtaa attgcctccc tgggcagaag gatgtggacg tggccttgaa gagcatcggg
     8521 gagtccagca agaagctgct tgtggattcg ctacctccaa gcacgaagcc tttccaggaa
     8581 gcccagagtg aactgaacca ggcagcagct gatctgaacc agtctgctgg ggaagtggtc
     8641 catgccaccc ggggccagag tggagagttg gctgcagcct ctggaaagtt cagtgatgat
     8701 tttgatgaat tcctcgatgc tggcattgag atggctggcc aagctcagac aaaagaagac
     8761 cagatccaag tgatagggaa cctcaagaat atctcgatgg catccagcaa gctgctgtta
     8821 gctgccaagt ctctctctgt agatccagga gctcccaatg cgaaaaatct cctggctgca
     8881 gctgcaagag ctgtgacaga gagcatcaat caactcatca ctctgtgtac ccaacaagct
     8941 ccgggccaga aagagtgcga taatgccctg cgggagctcg agactgtgaa ggggatgttg
     9001 gacaatccta atgaacctgt tagtgacctc tcttactttg actgcattga gagtgtgatg
     9061 gaaaactcca aggttctggg tgaatcgatg gcagggattc cacagaatgc caagaccgga
     9121 gacctccctg cctttgggga atgtgtgggg attgcatcca aggctctctg tgggctgaca
     9181 gaggctgcag cccaggctgc atacttggtt ggcatctctg atccaaacag ccaggcaggc
     9241 caccagggcc tggtggaccc catccagttt gccagggcta accaggccat ccagatggca
     9301 tgccagaact tggtggaccc tggcagcagc ccatcacagg tcctgtcagc cgccacaatt
     9361 gttgccaagc acacgtcagc cttgtgtaat gcctgccgca tcgcctcatc caagacggcc
     9421 aacccagtag ccaagaggca cttcgtccag tcagccaagg aagtcgccaa cagcactgcc
     9481 aacctggtga agaccatcaa ggccctggat ggggatttct ctgaagacaa ccgcaataag
     9541 tgtcgcatcg ccaccgcacc cttgattgaa gctgtggaga acctgacagc gttcgcctca
     9601 aaccctgagt ttgtcagcat tcctgcccag atcagctccg agggttccca ggcacaggaa
     9661 ccaatcctgg tctcagccaa gaccatgctg gagagttcat cgtacctcat tcgcactgcg
     9721 cgctctctgg ccatcaaccc caaagaccca cccacctggt ctgtactggc tggacattcc
     9781 catacagtgt ccgactccat caagagtctc atcacttcta tcagggacaa ggcccctgga
     9841 cagagggagt gtgattactc catcgatggc atcaaccggt gcatccggga catcgagcag
     9901 gcctcgctgg ccgccgtcag ccagagcctg gccacgaggg acgacatctc tgtggaggcc
     9961 ctgcaggagc agctgacttc ggtggtccag gaaatcggac accttatcga tcccatcgcc
    10021 acagcggctc ggggagaagc agctcagctg ggacataagg tgacacaact ggcaagctat
    10081 tttgagccct tgatcttagc cgcagttggt gtggcctcca agattcttga tcatcagcag
    10141 cagatgacgg tgctggacca gaccaagact ctcgcagagt ctgccttgca gatgttgtat
    10201 gcagccaaag aaggtggcgg aaaccccaag gcacaacaca cccatgacgc catcacagag
    10261 gccgcccagt tgatgaagga agccgtggat gacatcatgg tgacgctgaa cgaagctgcc
    10321 agtgaagtgg ggctggttgg gggcatggtg gacgccattg cagaagccat gagcaagctg
    10381 gatgaaggca ctcctccaga accaaaggga acatttgtcg actatcagac gactgtggtt
    10441 aaatactcca aagccattgc ggtgacagct caggaaatga tgactaagtc ggttactaac
    10501 ccggaggagt tgggaggact ggcttcacaa atgaccagtg actatgggca cctggctttc
    10561 cagggccaga tggcagcagc cacggcggaa ccagaggaga tcggattcca gattcgcact
    10621 cgtgtgcagg acctgggcca cggctgtatc ttcctggtgc agaaggcagg ggccctccag
    10681 gtctgcccca cagacagcta caccaagagg gagctgatcg aatgcgcccg tgccgtcacg
    10741 gaaaaggtct ccttggtgct ctcggctctc caggccggga acaaaggaac ccaggcatgc
    10801 attacagccg ccaccgctgt gtctgggatc attgccgacc tggacaccac cattatgttt
    10861 gcaacagcgg ggacgctgaa tgcagagaac agtgagacct tcgcagacca cagggagaac
    10921 attctcaaga cggccaaggc cttggtagaa gacacgaaac tacttgtgtc aggagctgcg
    10981 tccactcctg acaagctggc ccaggcggcc cagtcctcag cagccaccat cacccagctc
    11041 gcagaagtgg tcaagctggg ggcagccagc ctgggctccg acgaccccga gacccaggtg
    11101 gttttgatca atgccatcaa agatgtggcc aaggcccttt ctgatctcat cagtgctacc
    11161 aagggagctg ccagcaagcc agtggacgac ccttccatgt accagctcaa gggggctgcc
    11221 aaggtgatgg tgaccaatgt cacctcgctc ctcaagactg taaaggcagt ggaggatgag
    11281 gccacccggg gcaccagggc gcttgaggcc acaattgaat gcataaagca ggagcttacg
    11341 gtgttccagt caaaagacgt acctgaaaag acatcatcac ctgaagaatc cataaggatg
    11401 acgaaaggca tcaccatggc aacagccaaa gccgtggcag ctgggaactc atgtagacag
    11461 gaggacgtga ttgctactgc caacctgagc cggaaagccg tgtcagatat gttgacggct
    11521 tgcaagcaag catccttcca ccccgatgtc agtgacgagg tgagaaccag agccttgcgt
    11581 ttcgggacgg agtgcaccct tggctacttg gacctcctgg agcacgtctt ggtgattctt
    11641 cagaaaccaa ccccagaact caagcagcag ctggccgctt tctccaagcg agtcgccggc
    11701 gctgtgacag agctcatcca ggcggcggaa gccatgaaag gaacagagtg ggtggatcca
    11761 gaagacccaa ctgtcattgc agaaacagag ttactggggg ctgcagcatc catcgaagct
    11821 gctgctaaga agttagagca actgaagcca agagcaaaac caaaacaagc ggatgagacc
    11881 ctggactttg aggaacagat cttggaagct gctaaatcca ttgctgctgc cacaagcgcc
    11941 ctggtcaaat cggcctcagc agcccagagg gagctggtgg cccaaggaaa ggtgggctcc
    12001 atccctgcca acgctgcaga cgacggacag tggtcacaag ggctgatttc tgctgcccgg
    12061 atggtggcgg ctgcgaccag cagtctctgt gaggcggcca atgcctccgt tcagggacac
    12121 gccagcgagg agaagctcat ctcatctgcc aagcaggtcg ccgcttccac ggctcagctg
    12181 ctggtggcct gcaaggtgaa ggccgaccag gattcagagg ccatgaggcg gctacaggcg
    12241 gcaggaaatg ctgtgaaaag agcctcagac aatcttgtcc gtgcagccca gaaggcagca
    12301 tttggcaaag ctgatgacga cgatgttgta gtgaaaacca agtttgtggg gggcattgct
    12361 cagatcatcg ccgcccagga agaaatgcta aagaaagagc gagaactgga agaagcaagg
    12421 agaaaactgg cccaaatccg ccagcagcag tataagtttt tacccaccga gctgagggaa
    12481 gatgagggct gagaattcgc ggccgctcga gtctagaggg cccgcggttc gaacaaaaac
    12541 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt
    12601 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    12661 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    12721 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    12781 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    12841 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
    12901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    12961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    13021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    13081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    13141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    13201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    13261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    13321 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    13381 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    13441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    13501 cctaactccg cccatcccgc ccctaactc
//
