LOCUS       sequence-32557-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA, 
            Hafner M, Borkhardt A, Sander C, Tuschl T
  TITLE     RNA targets of wild-type and mutant FET family proteins.
  JOURNAL   Nat Struct Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163.
  PUBMED    22081015
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2011 Nov 13. doi: 10.1038/nsmb.2163."
COMMENT     Sequence Label: pDEST_EWSR1_G2A_R650H
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             170..196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    212..236
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgagctcgga tcgatccatc gatagtgtgg
      121 tggaattctg atcgccgccg ccatggacta caaggacgac gatgacaagt acccttatga
      181 cgtgcccgat tacgctttga accagatatc aacaagtttg tacaaaaaag caggctccac
      241 catgggaacc aattcagtcg acatggcgtc cacggattac agtacctata gccaagctgc
      301 agcgcagcag ggctacagtg cttacaccgc ccagcccact caaggatatg cacagaccac
      361 ccaggcatat gggcaacaaa gctatggaac ctatggacag cccactgatg tcagctatac
      421 ccaggctcag accactgcaa cctatgggca gaccgcctat gcaacttctt atggacagcc
      481 tcccactggt tatactactc caactgcccc ccaggcatac agccagcctg tccaggggta
      541 tggcactggt gcttatgata ccaccactgc tacagtcacc accacccagg cctcctatgc
      601 agctcagtct gcatatggca ctcagcctgc ttatccagcc tatgggcagc agccagcagc
      661 cactgcacct acaagaccgc aggatggaaa caagcccact gagactagtc aacctcaatc
      721 tagcacaggg ggttacaacc agcccagcct aggatatgga cagagtaact acagttatcc
      781 ccaggtacct ggga
//
