LOCUS       sequence-325637-       12242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj10a-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(162..186)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(189..763,764..766,767..775)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(356..373)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     583..602
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             782..802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(848..850,851..853,854..1567)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(848..850,851..853,854..1564)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(893..914)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1154..1173)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1501..1522
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1574..1593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1575..1593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1598..1732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1622..1641)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1676..1695
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2171..2358,2359..2370)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2404..2420)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2404..2420)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2457..2477)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2457..2475)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2496..2512)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2509..2531)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2520..2536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2544..2550,2551..2568,2569..2574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2589..2610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2727..2744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2898..3486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2978..2997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3657..4448,4449..4517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4280..4299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4518..4622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4785..4806)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5155..5166,5167..5329)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5702..5718
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5738..5758
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    11065..11089
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tacacaaaca ccacagcatc aaggcagcgg agaaaaaggg gcggatactg agaagatgcg
       61 attggaggag agctactggg aggaaagggg gcgggacaga ggccgtgtcc gagatcagct
      121 tagcgtacgc atcagtaacg tgtagaaggg tgggcgcgcc gacccagctt tcttgtacaa
      181 agtggccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
      241 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
      301 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      361 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      421 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      481 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      541 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      601 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      661 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
      721 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggc
      781 tccaaagaag aagcgtaagg taaatcgatc gaattcaagg cctctcgagc ctctagaagg
      841 tggcggaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      901 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      961 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1021 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1081 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1141 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1201 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1261 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1321 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1381 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1441 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1501 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1561 caagtaactg cagccctata gtgagtcgta ttacgtagat ccagacatga taagatacat
     1621 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1681 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1741 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1801 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctgcgaaga
     1861 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     1921 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     1981 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     2041 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     2101 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     2161 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     2221 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     2281 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     2341 gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca
     2401 gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     2461 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2521 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2581 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2641 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2701 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2761 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2821 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2881 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2941 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3001 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3061 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3121 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3241 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3301 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3361 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3421 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3481 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3541 cgttaaggca ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3601 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3661 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3721 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3781 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3841 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3901 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3961 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4021 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4081 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4141 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4201 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4261 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4321 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4381 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4441 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4501 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4561 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4621 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     4681 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4741 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     4801 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     4861 atggcccacg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     4921 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     4981 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     5041 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata tctgttcaga
     5101 cacccatatc cactctgttc cacacaggtc agaggtttgt ccaggagttc ttgacagagg
     5161 tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta
     5221 ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact
     5281 ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga
     5341 gattcttgtt taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat
     5401 cctgctttag aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag
     5461 tacacgctac tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa
     5521 attgttagtg tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag
     5581 cctctcacag gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat
     5641 agcaggaaac gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga
     5701 gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgaa
     5761 aattccagtt tgaagaaact tttcaagact caccttttca ctgttggtct taatttaggt
     5821 tgattatttt tgtcaattta ataattacat tgtattgttc atattttatt gtcttattgt
     5881 ggtgttttta cagctttttt attgttctgc tttgttactg tttgccttgt atgcattgta
     5941 gcgctttggg tctgagaaaa gcgcactata aataaactgt attattatta ttagggatgg
     6001 acatcacggt ggcgcgatgg gtggcacaat tacctcacag caagtaggtt gctgtttaga
     6061 gccccggctg gatcagttgg cagttctgtg tggagtttgc atgttctccc tgttcaagta
     6121 ggtttcctcc cggtgctctg gtttcccctc acaagtccaa agacatgatg tataggtgaa
     6181 tggggtaagc taaattgacc atagtgagtg tgtgtgtgtg tgtgaatgag agtgtatagg
     6241 tgtttcccag tgttaggttg caactggaag gacatctgct gtgtaaaaca tatgcaggat
     6301 aagttggcgg ttcattcccc tatggtgacc cctggttaat taaaggacta agttgaaaat
     6361 gaatgaatga atgaatgatt agagataaac cgataccact tttttacaaa ccgatatgag
     6421 tacaagtata atagtttctg ttactcgaca atactgagta ccgattctga tacttcttgg
     6481 atttggtttc aatgtaatta atttgaaaga aggttttatt tcttgcctgt cgcaaggatg
     6541 aacacaaaaa tctttaccag aaggatattt taagctaaga atatacgcac gttgttgaca
     6601 accctgtatc gattaccgat taaacgtgga catttaaagg ataagttatg cttgcgccat
     6661 cacaacttta cacattacat gatcaaagat catattgaat attcggttta ttcgtcagaa
     6721 gcctatagat ttgttaaaag tgtattttat aaacggatgg tttataaaga ctaagctatt
     6781 aatatatcag acattcatta atacatcaag acatcgatgt caggtaggac aacaaatcac
     6841 atccatgtaa tgaaatcttg ttgagtggta ttggtgcgtg gtttcagcat ttcatgtgca
     6901 atacgagtaa gaatatttta accaagtatc gacccaaaac ctgatactgg tatcggtgca
     6961 tcccttatta ttattattat caaaaacaca cttgttttat ttatgtgggt gccaaacaac
     7021 aaactatgaa atgcagcatg acacgcccaa aaagtgttcc tttgagaaca acttcataaa
     7081 tatgtttctt atcattatgc atgaatggtg tactgggaga gctttggtat cagaattgag
     7141 ataagagact ttggcattct aaaggacaat gacattatca cactgacagt caacaacact
     7201 ttattgctct ggaaatctta tgtcatgggg caaagctgag gttcattagg acagtacctt
     7261 atttagagat gaatgggaag gctgtagtgg gctgctatca gggaagggtg gtggtcctcg
     7321 ggggaactat ggactcacaa aagcacatct gctgtaacaa gatcccctgt gcaatctaca
     7381 aaagttacaa agagaagcat ggttggttaa aaaatagaat tgactggata tgctgaatgc
     7441 attgtttgtt gtgtgttaca tcacattaac cacagattgg tgctaaatat ttgcttatct
     7501 tataaaaacc tgtgtgttta taaagggggg tgtggatgga tgcttggcat tctttgaaaa
     7561 gatgttgggg gatggaagga ggagacctta ttgatgagta tccagtttgc agataacaag
     7621 tttttagaca ttcatactat gatgctgttt atgtctgttt tttctcattt tttctataca
     7681 caaacagcta taaaacattc acttttatat ctgatgctgt atattaaatt tcgttgtatt
     7741 tattctgttt gctctgttgg gtcatagcta gggccagaca gaatctgctg acattttttg
     7801 ctttttctgc agagaatttt gtaaacaatc tgtgatttat gcgagatttt ttcgggagta
     7861 tcgtaactaa aaacttacta tatgaaataa aaaaaaataa taccttttta acttttaata
     7921 aatatataca atgcaagtcc acctagatcc aattatttgg tcgacaaagc aagtctctca
     7981 cataatatat cttctaaaag acagaaaaca ttactttcag accatattgt aatcaaatga
     8041 acactttcat attagtcaat aatattacgg aaatgtattt aaaaactgaa taaacatata
     8101 tttacacctt tgtctttatt tctagagtgc ttttacaatg tagattgtgt caaagcagct
     8161 taacttagag gattagtata gtaaattgaa actgtgtcag tccatttttt aagagtttaa
     8221 gttcagttca gtttagttca gtgtgagtta atttttactg ctgagagtcc aaacactaaa
     8281 gagcaaatcc aaatacacaa gtcaataaat aaactcaatg atgggctaaa gatctgggga
     8341 aatctgcaga tttctgtgcg cacagattct gtgtgggcct agtcattacc tatactcaac
     8401 gtttactctg agaaacttcc atattttaga ggtaaaatgc tcagaattgt aaaaagtctt
     8461 gtcaaaatac ttggaatttg ccaaaaacaa aataaaagtc acatcacctc attgtccttg
     8521 gtatcagcaa gcacttttaa cacaatcagc tgtttttgtt tataactccc actgaaggcc
     8581 atgtgcatta ctcccaagtt acacagtaaa aagtcacatg tagtttatca tgatcactgc
     8641 tgctcaagca cagctttatc tgttattcag tgtggcaata tctgaaatgt gtgcaaaata
     8701 ttatttattg tagctgatca ccaacacttt cagtgttttt tttttctact tttaatgaga
     8761 attatgttct taggatttat ccttataaac ctctttaaaa agaaatgtta tgtctgcata
     8821 gacacgacat gaaattatga acagaaaatt taactattta agatcaagac ttgacatata
     8881 acatttctaa tgaacattaa acctgaggta ggcccacttg caaaaatgta actgctatca
     8941 ctcactttat acatcattgt gtaacttgta cacacaaaaa tagtgaagca atgtggcatg
     9001 gaatgcaggt cccattcatt caaagttatt aaagcacttt cttaaagtgc attactactg
     9061 agctaagacc agctagttgc tcatacaaca tactacaagt agtgtcatcc taatttggat
     9121 aaaaaaaatg tccattttat acatggaaaa gtactcatta gatcccccat tgtttgtatt
     9181 acggtatttc gtgaacacaa gaggtaaatg acctacagag ctgctgttgt gttagattga
     9241 aaacacaaca caggatcatg gaggcatcca tcattatgac gcattctgcc acttgtaact
     9301 ctgcacagac tttttgaaaa gtttaattga gtcttggcgt tgtcgcagaa aaatacgaaa
     9361 aattctgtgt taaaatttgc aaataaaaat tccagtttta taggaaatcg ccatggcctt
     9421 gttctttaac cagttaagct tccccttctt tcactctcaa gttgcaagaa gcaagtgtag
     9481 caatgtgcac gcgacagccg ggtgtgtgac gctggaccaa tcagagcaca gagctccgaa
     9541 agtttacctt ttatggctag agccgggtat gtgccgtcat ataaaagagc gcgcccagct
     9601 tttcagcctc actttgagct cctccacacg cagctagtgc ggaatatcat ctgcttgtaa
     9661 cccattctct taagtcgaca accccccaaa cccaaggtga gttggtttta cagtttttaa
     9721 gcgtttaatt atagtttcta tctattaatg ttaagtaaaa tgtgtaaatg gataaatatg
     9781 atttgatgaa gtctttagac agttcataac ggtctctttg tagttatcta ctaggtttga
     9841 tttataagtg gtggttcttt tgataatcta tgttacattt aataaatatt ggctgtatct
     9901 taactttttt gtagcgttag tggatgtggc aggtgagaat gtcggtaatt taacgtgacc
     9961 agtcgtaggc acgacattga acattgaata ggccggtgtg aaatgggtgt taagtcttaa
    10021 tataaagttg tgttctggct aatctctctt taacagccat aaatgtgatt tttaactaca
    10081 gttttgaggg tgctggtgag catgttgcac acttgatgga tagccggcat gggaagttct
    10141 ttgtgcaggc agtgctgcag cagggtgtga cctactttag ctagccggct aaccagctct
    10201 catctgatgt aacgtaaacc ccatcaatct aacatcggct cattcgtgct ttataagatc
    10261 gaagatcttg ttattagtaa cgggtattgg catttagttt taacttggtt aattttcttt
    10321 ttgttatgtg ttctatttaa atgtagcctg cagccccctc tgtctttaat gaagctgaag
    10381 attaacgtta acgttagtga agtgttgtat ccgctatgaa agatttgaat tttagaggtg
    10441 tggtccactt taataacggc tgcggagttc tctggctgca cttcagccgc tgtgactgtc
    10501 gaagcagagc ccatcagttt ttcaggatct cggtgatttt tgaggctaga ctatgaactg
    10561 aaccgactga gagtgaccgt ccagaaatgc tttagtgtga agtctccgct cacgaggagt
    10621 ccgtggactg actgcagatc tgtgacgcag taacacccgg cggcagacac ccgttgaaat
    10681 gcggttgtgt aattgatcgc aggcgagggt ggaagaggat gtgaaacttc atttgtgtaa
    10741 aatttaggga gtggtcctgg tgtgatgaat gtcgaaatct gttccttttt actgagccct
    10801 acgactctgg ctgagtgcca caccgccggc agccgcaaag cggctcaaac cattgccttt
    10861 tatggtaata atgagagaat gcagagggac ttcctttgtc tggcatatct gaggcgcgcg
    10921 ctgtcactag cgcccaccag cggtcagact gtagaatgca gagcaaaaca ggaagttgac
    10981 tccagatggt cacatgcttg ctgaaacgcc tctccctggc agcagtgcaa ttctaacagt
    11041 ttccctttct tttacagttc agcctcaagt ttgtacaaaa aagcaggctc cgcggccgcc
    11101 cccttcacca tgacttcagc cacgccccct tcctcccgta gctcctcccc ccagaaagtc
    11161 tgtcactccc agacacagac agatgtcctg aagcccctcc ttggtggcgg agggggcgga
    11221 acttccacag gaaacggccc tgcagctctg agacgccggc gacgggtttt gtccaaagat
    11281 gggcggagca acgttcgaat cgaacacgta agcggccgcg ggacacttta cctgcgggac
    11341 ctgtggacca ccttcttgga catgcagtgg cgctacaagc tcttcctgtt ttcggccacc
    11401 tttgctggca cctggtttgt ctttggggtc ctgtggtact tggtggccct cgtgcatgga
    11461 gatttactag agtttgatcc tccatccaac catacgccgt gtgtcatgca ggttcagact
    11521 ctcaccggag cgtttctttt ctctctggaa tcgcaaacta cgattggcta cggctttcgc
    11581 tgcattactg aggaatgtcc actcgccatc atcctgctga tcgttcagct cgtcatcaca
    11641 atggtgatgg agatcttcat cacgggaacc ttcctcgcca aggtagctcg gccgaagaag
    11701 cgtggcgaga cggtgaagtt cagtcagcat gctgtggtag ccaatcatga gggtcgaccc
    11761 tgtctcatga ttagagtggc caacatgcgc aagagcctgc ttctgggctg ccaggtatca
    11821 ggtaagctac tgcagacgtc gcagactaaa gagggggaga cggtgcgtct ggaccagagg
    11881 aatgtagcct tccaagtgga cacatctagt gacagtccct ttctcatcct acccctcact
    11941 ttctaccatg tgatagacga caacagccca ctgagggcat gggccgccaa aggtggtggg
    12001 tggacggatc ctgaactggc tgactttgag ctgctggtga tcatgagcgc tacagttgaa
    12061 ccgacctcgg ccacatgtca ggtgcgcacc tcatacctgc cggacgagat cctgtggggc
    12121 tacgagtttc cacccgtcgt ctctctctca ccctctggaa aatacgtcgc cgatttcgct
    12181 ttcttcgaca aggttgccaa gaccaagact ccgccccttt tcaaacaagc ccctccctcc
    12241 ca
//
