LOCUS       sequence-325639-        4819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self complementary AAV vector for rapid SYFP2 expression in 
            forebrain GABAergic interneurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4819)
  TITLE     Enhancer AAVs from the Allen Institute
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP11203-scAAV-hDLX-minBG-SYFP2-WPRE3-BGHpA (A...
FEATURES             Location/Qualifiers
     source          1..4819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(141..157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(141..157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(154..176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(189..195,196..213,214..219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    234..255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(543..1131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(623..642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1302..2093,2094..2162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1925..1944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2163..2267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2335..2353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2391..2413)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2513..2532
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2726..2748
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2740..2757
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2741..2757
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2996..3107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3287..3307
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(3400..4110,4111..4113,4114..4116))
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3442..3463)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3791..3810
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4050..4071
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4113..4122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctctgcgc gctcgctcgc tcactgaggc cgcccgggct ttgcccgggc ggcctcagtg
       61 agcgagcgag cgcgcagaga gggagtggcc cagatccccc cgggaagctt tctagatctc
      121 gagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      181 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      241 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      301 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      421 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2281 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2341 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2401 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2461 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2521 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2581 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2641 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2701 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2761 ttgtcgacgc atgcgggccc ggatctgtcc ctctctgcgc gctcgctcgc tcactgaggc
     2821 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
     2881 agcgcgcaga gagggagtgg actagtcatg cctgctattg tcttcccaat cctccccctt
     2941 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     3001 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     3061 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg ctcgagccac
     3121 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     3181 gagttccgcc gtggcaagaa ctaaccagga tttatacaag gaggagaaaa tgaaagccat
     3241 acgggaagca atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg
     3301 agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt
     3361 atgatatcga attcgcggcc gcggcgcgcc gtcgacttac ttgtacagct cgtccatgcc
     3421 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
     3481 ggggtctttg ctcagcttgg actggtagct caggtagtgg ttgtcgggca gcagcacggg
     3541 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc
     3601 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
     3661 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
     3721 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     3781 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
     3841 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
     3901 gaagcactgc acgccgtagc ccagggtggt cacgagggtg ggccagggca cgggcagctt
     3961 gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
     4021 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
     4081 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggta gcgctagctt
     4141 cgaaagatct ggatcccaga agcaaatgta agcaatagat ggctctgccc tgacttttat
     4201 gcccagcccg agctccctac tgtgaaactt tgggttcgta gctccccagg atcaattctg
     4261 aacaaagcct ccagctgcag tgccatccaa tttgaagcag acattgggga caatttaagg
     4321 tttttatcca caagaaggtt tttttccatt ctcttaaatg cagccataat tagagtaatt
     4381 tttcatgtag cccgctgatt acagcgtttt taccgtcaaa gataattacc tgtaattttc
     4441 ttccactttt aatactaaaa agccatcttt atttagattc aggaacagga aaggcgaaac
     4501 aaaagaggga aattattctg ttattcatac acaaattgca gagacgtagg acctaaaatt
     4561 gaaaattaac caaaattata atgctgaaaa gaatggaaga ggctgcagaa gtacagctgg
     4621 tagtggggct ttgctgaatt attcaaatta cgttagggag aatgctacca tacatcgaaa
     4681 ccaacactaa tttttcgtta aaaaaaattc accagatgca tgccaaacca ctgtgagtgc
     4741 atagagctcg gtacctatac gcgtcctagg tcacgactcc acccctccag gaacccctag
     4801 tgatggagtt ggccactcc
//
