LOCUS       sequence-325641-       11439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 
            fused with EGFP at the C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 11439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-EGFP
FEATURES             Location/Qualifiers
     source          1..11439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1309..1333)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1332..1341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1338..1340,1341..1343,1344..2054)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1338..1340,1341..1343,1344..2054)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1383..1404)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1644..1663)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1991..2012
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2107..2126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2108..2126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2131..2265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2155..2174)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2209..2228
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2704..2891,2892..2903)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2937..2953)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2937..2953)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2990..3010)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2990..3008)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3029..3045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3042..3064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3053..3069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3077..3083,3084..3101,3102..3107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3122..3143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     gap             3242..3261
                     /estimated_length=20
     rep_origin      complement(3431..4019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3511..3530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4190..4981,4982..5050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4813..4832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5051..5155)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5318..5339)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5688..5699,5700..5862)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6235..6251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6271..6291
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atctgaggcg cgcgctgtca ctagcgccca ccagcggtca gactgtagaa tgcagagcaa
       61 aacaggaagt tgactccaga tggtcacatg cttgctgaaa cgcctctccc tggcagcagt
      121 gcaattctaa cagtttccct ttcttttaca gttcagcctc aagtttgtac aaaaaagcag
      181 gctccgcggc cgcccccttc accatgccta ccaccatgac aaacaccacc gctgaccaaa
      241 aggcctcctg ccctttgatg gtcaaacctc agcgtcgccg cctggtcagc aaagacggtc
      301 ggagccagac aagaggaaac acaagaggag gctccagaga gacgtgtttc agtgctctgc
      361 gggacttgtg gggcacatgg ctggcgctac ggtggcggtg ggtggttttg gcattttgtg
      421 gctcattcct gcttcactgg ctgctgtttg ctgtattatg gtacctgctg gctcgggtca
      481 atggagacct ggatgtcctg gatcatgatt ctcctcctcc cggtcatgtg ctgtgtgtta
      541 aacatgtgaa tgggttcaca gccgcttttt cattcgccct ggagacgcaa ctcactattg
      601 ggtatggcac catgtaccca aatgcagact gtcccactgc catcgcgctg cttgccctgc
      661 agatgctgct gggactcatg ctggaggcct ttatcactgg tgcatttgta gccaagttct
      721 ctcggcctca gaagcgatgt gacgggatcc tattcagccc tcaggctgtg gtgtgtgagc
      781 agaaaggcca gcgctgtcta atgtttcgag tgtgtaacct ccagccacag ccgctagtgg
      841 acgtatccgt gagtgctgtc ctctacgagg agcgagacga tcacgaactc caccaaactg
      901 ctctggagtt cagcatcgac aacctgggct ccagatcttg ccctcttttc ctctcccctc
      961 tgaccttctt tcacccttta aatccatcca ctcctttcat caacaaccca agcagccaaa
     1021 cacactttga gcttgtagtg tttctcactg cgactcagga aagcacaggg tctggttacc
     1081 acaagaggac gtcttacctg cctgacgaga tccaatatgg ctactgcttc tccaaggtga
     1141 cgtctgtaca ccagaacaaa accccaaaca tgcgctattt tgacacggtg ccctgtcccc
     1201 tcacactagc caacacacac accactgata caccggataa agagcatgtg gtggttcagc
     1261 tgaatggaga aggcagcgac cgcgtggaga agaagggtgg gcgcgccgac ccagctttct
     1321 tgtacaaagt ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1381 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1441 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1501 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1561 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1621 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1681 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1741 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1801 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1861 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1921 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1981 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2041 acgagctgta caagggcggt ggaagatctg ggaattcaag gcctctcgag cctctagatt
     2101 ctgcagccct atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     2161 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2221 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     2281 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2341 ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga agatacggcc
     2401 acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat
     2461 cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt
     2521 caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga
     2581 acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat
     2641 ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga
     2701 atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa
     2761 aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt
     2821 gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat
     2881 tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc
     2941 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     3001 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3181 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3241 tnnnnnnnnn nnnnnnnnnn ncgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4081 gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4141 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4201 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4261 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4321 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4381 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4441 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4501 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4561 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4621 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4681 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4741 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4801 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4861 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4921 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4981 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5041 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5101 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5161 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     5221 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     5281 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5341 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     5401 acgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat
     5461 tggccacaat gacggctact acatggtgcc attccttcct ctttatagga atggagacta
     5521 cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg
     5581 cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat
     5641 atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa
     5701 agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt
     5761 aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa
     5821 aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt
     5881 gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt
     5941 tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc
     6001 tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta
     6061 gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca
     6121 caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga
     6181 aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccct cgagcaggaa
     6241 acagctatga ccatgattac gccaagctat caactttgta tagaaaagtt gaaaattcca
     6301 gtttgaagaa acttttcaag actcaccttt tcactgttgg tcttaattta ggttgattat
     6361 ttttgtcaat ttaataatta cattgtattg ttcatatttt attgtcttat tgtggtgttt
     6421 ttacagcttt tttattgttc tgctttgtta ctgtttgcct tgtatgcatt gtagcgcttt
     6481 gggtctgaga aaagcgcact ataaataaac tgtattatta ttattaggga tggacatcac
     6541 ggtggcgcga tgggtggcac aattacctca cagcaagtag gttgctgttt agagccccgg
     6601 ctggatcagt tggcagttct gtgtggagtt tgcatgttct ccctgttcaa gtaggtttcc
     6661 tcccggtgct ctggtttccc ctcacaagtc caaagacatg atgtataggt gaatggggta
     6721 agctaaattg accatagtga gtgtgtgtgt gtgtgtgaat gagagtgtat aggtgtttcc
     6781 cagtgttagg ttgcaactgg aaggacatct gctgtgtaaa acatatgcag gataagttgg
     6841 cggttcattc ccctatggtg acccctggtt aattaaagga ctaagttgaa aatgaatgaa
     6901 tgaatgaatg attagagata aaccgatacc acttttttac aaaccgatat gagtacaagt
     6961 ataatagttt ctgttactcg acaatactga gtaccgattc tgatacttct tggatttggt
     7021 ttcaatgtaa ttaatttgaa agaaggtttt atttcttgcc tgtcgcaagg atgaacacaa
     7081 aaatctttac cagaaggata ttttaagcta agaatatacg cacgttgttg acaaccctgt
     7141 atcgattacc gattaaacgt ggacatttaa aggataagtt atgcttgcgc catcacaact
     7201 ttacacatta catgatcaaa gatcatattg aatattcggt ttattcgtca gaagcctata
     7261 gatttgttaa aagtgtattt tataaacgga tggtttataa agactaagct attaatatat
     7321 cagacattca ttaatacatc aagacatcga tgtcaggtag gacaacaaat cacatccatg
     7381 taatgaaatc ttgttgagtg gtattggtgc gtggtttcag catttcatgt gcaatacgag
     7441 taagaatatt ttaaccaagt atcgacccaa aacctgatac tggtatcggt gcatccctta
     7501 ttattattat tatcaaaaac acacttgttt tatttatgtg ggtgccaaac aacaaactat
     7561 gaaatgcagc atgacacgcc caaaaagtgt tcctttgaga acaacttcat aaatatgttt
     7621 cttatcatta tgcatgaatg gtgtactggg agagctttgg tatcagaatt gagataagag
     7681 actttggcat tctaaaggac aatgacatta tcacactgac agtcaacaac actttattgc
     7741 tctggaaatc ttatgtcatg gggcaaagct gaggttcatt aggacagtac cttatttaga
     7801 gatgaatggg aaggctgtag tgggctgcta tcagggaagg gtggtggtcc tcgggggaac
     7861 tatggactca caaaagcaca tctgctgtaa caagatcccc tgtgcaatct acaaaagtta
     7921 caaagagaag catggttggt taaaaaatag aattgactgg atatgctgaa tgcattgttt
     7981 gttgtgtgtt acatcacatt aaccacagat tggtgctaaa tatttgctta tcttataaaa
     8041 acctgtgtgt ttataaaggg gggtgtggat ggatgcttgg cattctttga aaagatgttg
     8101 ggggatggaa ggaggagacc ttattgatga gtatccagtt tgcagataac aagtttttag
     8161 acattcatac tatgatgctg tttatgtctg ttttttctca ttttttctat acacaaacag
     8221 ctataaaaca ttcactttta tatctgatgc tgtatattaa atttcgttgt atttattctg
     8281 tttgctctgt tgggtcatag ctagggccag acagaatctg ctgacatttt ttgctttttc
     8341 tgcagagaat tttgtaaaca atctgtgatt tatgcgagat tttttcggga gtatcgtaac
     8401 taaaaactta ctatatgaaa taaaaaaaaa taataccttt ttaactttta ataaatatat
     8461 acaatgcaag tccacctaga tccaattatt tggtcgacaa agcaagtctc tcacataata
     8521 tatcttctaa aagacagaaa acattacttt cagaccatat tgtaatcaaa tgaacacttt
     8581 catattagtc aataatatta cggaaatgta tttaaaaact gaataaacat atatttacac
     8641 ctttgtcttt atttctagag tgcttttaca atgtagattg tgtcaaagca gcttaactta
     8701 gaggattagt atagtaaatt gaaactgtgt cagtccattt tttaagagtt taagttcagt
     8761 tcagtttagt tcagtgtgag ttaattttta ctgctgagag tccaaacact aaagagcaaa
     8821 tccaaataca caagtcaata aataaactca atgatgggct aaagatctgg ggaaatctgc
     8881 agatttctgt gcgcacagat tctgtgtggg cctagtcatt acctatactc aacgtttact
     8941 ctgagaaact tccatatttt agaggtaaaa tgctcagaat tgtaaaaagt cttgtcaaaa
     9001 tacttggaat ttgccaaaaa caaaataaaa gtcacatcac ctcattgtcc ttggtatcag
     9061 caagcacttt taacacaatc agctgttttt gtttataact cccactgaag gccatgtgca
     9121 ttactcccaa gttacacagt aaaaagtcac atgtagttta tcatgatcac tgctgctcaa
     9181 gcacagcttt atctgttatt cagtgtggca atatctgaaa tgtgtgcaaa atattattta
     9241 ttgtagctga tcaccaacac tttcagtgtt ttttttttct acttttaatg agaattatgt
     9301 tcttaggatt tatccttata aacctcttta aaaagaaatg ttatgtctgc atagacacga
     9361 catgaaatta tgaacagaaa atttaactat ttaagatcaa gacttgacat ataacatttc
     9421 taatgaacat taaacctgag gtaggcccac ttgcaaaaat gtaactgcta tcactcactt
     9481 tatacatcat tgtgtaactt gtacacacaa aaatagtgaa gcaatgtggc atggaatgca
     9541 ggtcccattc attcaaagtt attaaagcac tttcttaaag tgcattacta ctgagctaag
     9601 accagctagt tgctcataca acatactaca agtagtgtca tcctaatttg gataaaaaaa
     9661 atgtccattt tatacatgga aaagtactca ttagatcccc cattgtttgt attacggtat
     9721 ttcgtgaaca caagaggtaa atgacctaca gagctgctgt tgtgttagat tgaaaacaca
     9781 acacaggatc atggaggcat ccatcattat gacgcattct gccacttgta actctgcaca
     9841 gactttttga aaagtttaat tgagtcttgg cgttgtcgca gaaaaatacg aaaaattctg
     9901 tgttaaaatt tgcaaataaa aattccagtt ttataggaaa tcgccatggc cttgttcttt
     9961 aaccagttaa gcttcccctt ctttcactct caagttgcaa gaagcaagtg tagcaatgtg
    10021 cacgcgacag ccgggtgtgt gacgctggac caatcagagc acagagctcc gaaagtttac
    10081 cttttatggc tagagccggg tatgtgccgt catataaaag agcgcgccca gcttttcagc
    10141 ctcactttga gctcctccac acgcagctag tgcggaatat catctgcttg taacccattc
    10201 tcttaagtcg acaacccccc aaacccaagg tgagttggtt ttacagtttt taagcgttta
    10261 attatagttt ctatctatta atgttaagta aaatgtgtaa atggataaat atgatttgat
    10321 gaagtcttta gacagttcat aacggtctct ttgtagttat ctactaggtt tgatttataa
    10381 gtggtggttc ttttgataat ctatgttaca tttaataaat attggctgta tcttaacttt
    10441 tttgtagcgt tagtggatgt ggcaggtgag aatgtcggta atttaacgtg accagtcgta
    10501 ggcacgacat tgaacattga ataggccggt gtgaaatggg tgttaagtct taatataaag
    10561 ttgtgttctg gctaatctct ctttaacagc cataaatgtg atttttaact acagttttga
    10621 gggtgctggt gagcatgttg cacacttgat ggatagccgg catgggaagt tctttgtgca
    10681 ggcagtgctg cagcagggtg tgacctactt tagctagccg gctaaccagc tctcatctga
    10741 tgtaacgtaa accccatcaa tctaacatcg gctcattcgt gctttataag atcgaagatc
    10801 ttgttattag taacgggtat tggcatttag ttttaacttg gttaattttc tttttgttat
    10861 gtgttctatt taaatgtagc ctgcagcccc ctctgtcttt aatgaagctg aagattaacg
    10921 ttaacgttag tgaagtgttg tatccgctat gaaagatttg aattttagag gtgtggtcca
    10981 ctttaataac ggctgcggag ttctctggct gcacttcagc cgctgtgact gtcgaagcag
    11041 agcccatcag tttttcagga tctcggtgat ttttgaggct agactatgaa ctgaaccgac
    11101 tgagagtgac cgtccagaaa tgctttagtg tgaagtctcc gctcacgagg agtccgtgga
    11161 ctgactgcag atctgtgacg cagtaacacc cggcggcaga cacccgttga aatgcggttg
    11221 tgtaattgat cgcaggcgag ggtggaagag gatgtgaaac ttcatttgtg taaaatttag
    11281 ggagtggtcc tggtgtgatg aatgtcgaaa tctgttcctt tttactgagc cctacgactc
    11341 tggctgagtg ccacaccgcc ggcagccgca aagcggctca aaccattgcc ttttatggta
    11401 ataatgagag aatgcagagg gacttccttt gtctggcat
//
