LOCUS       sequence-325648-        5309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for Cre expression in forebrain GABAergic interneurons. 
            For use in combination with Cre-dependent transgenic mouse lines or 
            co-infection with Cre dependent viral vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA...
FEATURES             Location/Qualifiers
     source          1..5309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2136..2156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2343..2454
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2572..2712
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2572..2701
                     /label=AAV2 ITR
     rep_origin      2787..3242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2874..2893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3084..3105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3259..3278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3378..3400
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3438..3456)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3524..3628
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3629..3697,3698..4489)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3847..3866)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4660..5248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5149..5168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataggt accgagctct atgcactcac agtggtttgg
      181 catgcatctg gtgaattttt tttaacgaaa aattagtgtt ggtttcgatg tatggtagca
      241 ttctccctaa cgtaatttga ataattcagc aaagccccac taccagctgt acttctgcag
      301 cctcttccat tcttttcagc attataattt tggttaattt tcaattttag gtcctacgtc
      361 tctgcaattt gtgtatgaat aacagaataa tttccctctt ttgtttcgcc tttcctgttc
      421 ctgaatctaa ataaagatgg ctttttagta ttaaaagtgg aagaaaatta caggtaatta
      481 tctttgacgg taaaaacgct gtaatcagcg ggctacatga aaaattactc taattatggc
      541 tgcatttaag agaatggaaa aaaaccttct tgtggataaa aaccttaaat tgtccccaat
      601 gtctgcttca aattggatgg cactgcagct ggaggctttg ttcagaattg atcctgggga
      661 gctacgaacc caaagtttca cagtagggag ctcgggctgg gcataaaagt cagggcagag
      721 ccatctattg cttacatttg cttctgggat ccagatcttt cgaagctagc accatggtgc
      781 ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca ccaaaacctg cctgccctcc
      841 ctgtggatgc cacctctgat gaagtcagga agaacctgat ggacatgttc agggacaggc
      901 aggccttctc tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc tgggctgcct
      961 ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg agggactacc
     1021 tcctgtacct gcaagccaga ggcctggctg tgaagaccat ccaacagcac ctgggccagc
     1081 tcaacatgct gcacaggaga tctggcctgc ctcgcccttc tgactccaat gctgtgtccc
     1141 tggtgatgag gagaatcaga aaggagaatg tggatgctgg ggagagagcc aagcaggccc
     1201 tggcctttga acgcactgac tttgaccaag tcagatccct gatggagaac tctgacagat
     1261 gccaggacat caggaacctg gccttcctgg gcattgccta caacaccctg ctgcgcattg
     1321 ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac cgatggtggg agaatgctga
     1381 tccacattgg caggaccaag accctggtgt ccacagctgg tgtggagaag gccctgtccc
     1441 tgggggttac caagctggtg gagagatgga tctctgtgtc tggtgtggct gatgacccca
     1501 acaactacct gttctgccgg gtcagaaaga atggtgtggc tgccccttct gccacctccc
     1561 aactgtccac ccgggccctg gaagggatct ttgaggccac ccaccgcctg atctatggtg
     1621 ccaaggatga ctctgggcag agatacctgg cctggtctgg ccactctgcc agagtgggtg
     1681 ctgccaggga catggccagg gctggtgtgt ccatccctga aatcatgcag gctggtggct
     1741 ggaccaatgt gaacattgtg atgaactaca tcagaaacct ggactctgag actggggcca
     1801 tggtgaggct gctcgaggat ggggactaat gaggcgcgcc gcggccttaa agagaccggt
     1861 tcactgtgac agtaaaagag accggttcac tgtgagaatg aaagagaccg gttcactgtg
     1921 atcggaaaag agaccggttc actgtgagcg gccttgaaac ccagcagaca atgtagctca
     1981 gtagaaaccc agcagacaat gtagctgaat ggaaacccag cagacaatgt agcttcggag
     2041 aaacccagca gacaatgtag ctgtcgacga attcgatatc ataatcaacc tctggattac
     2101 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2161 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2221 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     2281 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggctcg agagatcttc
     2341 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2401 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2461 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2521 ttgggaagac aatagcaggc atgagatctc acgtgcggac cgagcggccg caggaacccc
     2581 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     2641 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     2701 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2761 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     2821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     2881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3001 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3061 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     3121 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     3181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3241 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3301 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3361 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3421 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3481 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3541 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3601 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3661 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3721 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3781 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3841 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3901 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3961 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4021 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4081 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4141 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4201 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4261 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4321 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4381 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4441 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4501 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4561 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4621 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4681 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4741 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4801 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4861 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4921 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4981 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5041 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5101 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5161 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5221 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5281 ggccttttgc tggccttttg ctcacatgt
//
