LOCUS       sequence-32565-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pQCXIR (w498-1)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(152..160,161..163,164..738)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(325..344)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     554..571
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc
       61 cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga
      121 actcggtgat gacgttctcg gaggaggcca tggttgtggc catattatca tcgtgttttt
      181 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa
      241 acacatgtaa agcatgtgta ccgaggcccc agatcagatc ccatacaatg gggtaccttc
      301 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca
      361 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca
      421 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg
      481 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca
      541 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag
      601 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat
      661 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg
      721 gggggggagg gagangggcg gatccgttaa ttaagcgtac gaggcctacc ggtgcggccg
      781 cggatcaatt cctgcaggtc cgttccaatg caccgttccc ggccgcggag gctggatcgg
      841 tcccggtgtc ttctatggag gtcaaaa
//
