LOCUS       sequence-32570-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN21-R4R3
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     616..638
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(676..694)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatga gctagccggc catacaagta
       61 atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt
      121 ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa
      181 ttcaatccaa atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc
      241 tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt
      301 ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac
      361 acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa
      421 atatatagag gaggaaggaa aactagttct agagcggccg ccaccgcggt ggagctccca
      481 tatggtgcac tctcagtgca atctgctctg atgccgcata gttaagccag ccccgacacc
      541 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      601 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      661 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      721 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaa
//
