LOCUS       sequence-325713-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optimized EKAREV FRET biosensor sensor for ERK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ponsioen B, Post JB, Buissant des Amorie JR, Laskaris D, van Ineveld
            RL, Kersten S, Bertotti A, Sassi F, Sipieter F, Cappe B, Mertens S, 
            Verlaan-Klink I, Boj SF, Vries RGJ, Rehmann H, Vandenabeele P, 
            Riquet FB, Trusolino L, Bos JL, Snippert HJG
  TITLE     Quantifying single-cell ERK dynamics in colorectal cancer organoids 
            reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.
  JOURNAL   Nat Cell Biol. 2021 Apr;23(4):377-390. doi: 
            10.1038/s41556-021-00654-5. Epub 2021 Apr 1.
  PUBMED    33795873
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-021-00654-5"; journalName: "Nat Cell Biol"; date: 
            "2021-04"; volume: "23"; issue: "4"; pages: "377-390"
COMMENT     Sequence Label: pLV x EF1a_EKAREN5-ires-blast
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    44..596
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(188..205)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     415..434
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      617..626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             623..1021
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 catttgttca gtggcccggg aattccgccc ctctcccccc ctaacgttac tggccgaagc
       61 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
      121 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      181 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      241 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      301 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      361 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      421 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      481 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
      541 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatgga
      601 tatggccaca acggtcgcca ccatggccaa gcctttgtct caagaagaat ccaccctcat
      661 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
      721 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
      781 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
      841 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
      901 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
      961 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     1021 a
//
