LOCUS       sequence-32573-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN22-R4R3
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..263)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(271..288)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(271..287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(280..302)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcaacttta ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg
      301 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      361 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      421 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      481 atgccgggcc ctgtgtgcgg ttttttctat gatgtttcaa aaaaatttat cagaagtaaa
      541 aaacataaga agtgtacaca gaacattgtg tagattttgg gatagaatta ttattaatgg
      601 ggggagggag cacaattttt ttcgactttt tattacgaat taattctatt tttgaatcta
      661 cacaatttca ttgttagagg tgacttaaaa gaagctaaaa aacaaagaaa ttaagagaag
      721 ttttttgata ataacaaaaa tagggggtgg gagcacaggg agaaagagca tgtagggatg
      781 ttgaagagta attggactta gaagtcagag gcacgggcgc gagatggcga tctgatgaca
//
