LOCUS       sequence-325735-       12443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 12443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcna1a-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5025..5049
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6565..6589)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6592..7166,7167..7169,7170..7178)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6759..6776)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6986..7005
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7185..7205
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7251..7253,7254..7256,7257..7970)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7251..7253,7254..7256,7257..7967)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7296..7317)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7557..7576)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7904..7925
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7977..7996)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7978..7996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8001..8135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8025..8044)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8079..8098
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8574..8761,8762..8773)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8807..8823)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8807..8823)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8860..8880)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8860..8878)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8899..8915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8912..8934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8923..8939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8947..8953,8954..8971,8972..8977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8992..9013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9130..9147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9301..9889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9381..9400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10060..10851,10852..10920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10683..10702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10921..11025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11188..11209)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(11558..11569,11570..11732)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     12105..12121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12141..12161
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtaggtt gctgtttaga gccccggctg gatcagttgg cagttctgtg tggagtttgc
       61 atgttctccc tgttcaagta ggtttcctcc cggtgctctg gtttcccctc acaagtccaa
      121 agacatgatg tataggtgaa tggggtaagc taaattgacc atagtgagtg tgtgtgtgtg
      181 tgtgaatgag agtgtatagg tgtttcccag tgttaggttg caactggaag gacatctgct
      241 gtgtaaaaca tatgcaggat aagttggcgg ttcattcccc tatggtgacc cctggttaat
      301 taaaggacta agttgaaaat gaatgaatga atgaatgatt agagataaac cgataccact
      361 tttttacaaa ccgatatgag tacaagtata atagtttctg ttactcgaca atactgagta
      421 ccgattctga tacttcttgg atttggtttc aatgtaatta atttgaaaga aggttttatt
      481 tcttgcctgt cgcaaggatg aacacaaaaa tctttaccag aaggatattt taagctaaga
      541 atatacgcac gttgttgaca accctgtatc gattaccgat taaacgtgga catttaaagg
      601 ataagttatg cttgcgccat cacaacttta cacattacat gatcaaagat catattgaat
      661 attcggttta ttcgtcagaa gcctatagat ttgttaaaag tgtattttat aaacggatgg
      721 tttataaaga ctaagctatt aatatatcag acattcatta atacatcaag acatcgatgt
      781 caggtaggac aacaaatcac atccatgtaa tgaaatcttg ttgagtggta ttggtgcgtg
      841 gtttcagcat ttcatgtgca atacgagtaa gaatatttta accaagtatc gacccaaaac
      901 ctgatactgg tatcggtgca tcccttatta ttattattat caaaaacaca cttgttttat
      961 ttatgtgggt gccaaacaac aaactatgaa atgcagcatg acacgcccaa aaagtgttcc
     1021 tttgagaaca acttcataaa tatgtttctt atcattatgc atgaatggtg tactgggaga
     1081 gctttggtat cagaattgag ataagagact ttggcattct aaaggacaat gacattatca
     1141 cactgacagt caacaacact ttattgctct ggaaatctta tgtcatgggg caaagctgag
     1201 gttcattagg acagtacctt atttagagat gaatgggaag gctgtagtgg gctgctatca
     1261 gggaagggtg gtggtcctcg ggggaactat ggactcacaa aagcacatct gctgtaacaa
     1321 gatcccctgt gcaatctaca aaagttacaa agagaagcat ggttggttaa aaaatagaat
     1381 tgactggata tgctgaatgc attgtttgtt gtgtgttaca tcacattaac cacagattgg
     1441 tgctaaatat ttgcttatct tataaaaacc tgtgtgttta taaagggggg tgtggatgga
     1501 tgcttggcat tctttgaaaa gatgttgggg gatggaagga ggagacctta ttgatgagta
     1561 tccagtttgc agataacaag tttttagaca ttcatactat gatgctgttt atgtctgttt
     1621 tttctcattt tttctataca caaacagcta taaaacattc acttttatat ctgatgctgt
     1681 atattaaatt tcgttgtatt tattctgttt gctctgttgg gtcatagcta gggccagaca
     1741 gaatctgctg acattttttg ctttttctgc agagaatttt gtaaacaatc tgtgatttat
     1801 gcgagatttt ttcgggagta tcgtaactaa aaacttacta tatgaaataa aaaaaaataa
     1861 taccttttta acttttaata aatatataca atgcaagtcc acctagatcc aattatttgg
     1921 tcgacaaagc aagtctctca cataatatat cttctaaaag acagaaaaca ttactttcag
     1981 accatattgt aatcaaatga acactttcat attagtcaat aatattacgg aaatgtattt
     2041 aaaaactgaa taaacatata tttacacctt tgtctttatt tctagagtgc ttttacaatg
     2101 tagattgtgt caaagcagct taacttagag gattagtata gtaaattgaa actgtgtcag
     2161 tccatttttt aagagtttaa gttcagttca gtttagttca gtgtgagtta atttttactg
     2221 ctgagagtcc aaacactaaa gagcaaatcc aaatacacaa gtcaataaat aaactcaatg
     2281 atgggctaaa gatctgggga aatctgcaga tttctgtgcg cacagattct gtgtgggcct
     2341 agtcattacc tatactcaac gtttactctg agaaacttcc atattttaga ggtaaaatgc
     2401 tcagaattgt aaaaagtctt gtcaaaatac ttggaatttg ccaaaaacaa aataaaagtc
     2461 acatcacctc attgtccttg gtatcagcaa gcacttttaa cacaatcagc tgtttttgtt
     2521 tataactccc actgaaggcc atgtgcatta ctcccaagtt acacagtaaa aagtcacatg
     2581 tagtttatca tgatcactgc tgctcaagca cagctttatc tgttattcag tgtggcaata
     2641 tctgaaatgt gtgcaaaata ttatttattg tagctgatca ccaacacttt cagtgttttt
     2701 tttttctact tttaatgaga attatgttct taggatttat ccttataaac ctctttaaaa
     2761 agaaatgtta tgtctgcata gacacgacat gaaattatga acagaaaatt taactattta
     2821 agatcaagac ttgacatata acatttctaa tgaacattaa acctgaggta ggcccacttg
     2881 caaaaatgta actgctatca ctcactttat acatcattgt gtaacttgta cacacaaaaa
     2941 tagtgaagca atgtggcatg gaatgcaggt cccattcatt caaagttatt aaagcacttt
     3001 cttaaagtgc attactactg agctaagacc agctagttgc tcatacaaca tactacaagt
     3061 agtgtcatcc taatttggat aaaaaaaatg tccattttat acatggaaaa gtactcatta
     3121 gatcccccat tgtttgtatt acggtatttc gtgaacacaa gaggtaaatg acctacagag
     3181 ctgctgttgt gttagattga aaacacaaca caggatcatg gaggcatcca tcattatgac
     3241 gcattctgcc acttgtaact ctgcacagac tttttgaaaa gtttaattga gtcttggcgt
     3301 tgtcgcagaa aaatacgaaa aattctgtgt taaaatttgc aaataaaaat tccagtttta
     3361 taggaaatcg ccatggcctt gttctttaac cagttaagct tccccttctt tcactctcaa
     3421 gttgcaagaa gcaagtgtag caatgtgcac gcgacagccg ggtgtgtgac gctggaccaa
     3481 tcagagcaca gagctccgaa agtttacctt ttatggctag agccgggtat gtgccgtcat
     3541 ataaaagagc gcgcccagct tttcagcctc actttgagct cctccacacg cagctagtgc
     3601 ggaatatcat ctgcttgtaa cccattctct taagtcgaca accccccaaa cccaaggtga
     3661 gttggtttta cagtttttaa gcgtttaatt atagtttcta tctattaatg ttaagtaaaa
     3721 tgtgtaaatg gataaatatg atttgatgaa gtctttagac agttcataac ggtctctttg
     3781 tagttatcta ctaggtttga tttataagtg gtggttcttt tgataatcta tgttacattt
     3841 aataaatatt ggctgtatct taactttttt gtagcgttag tggatgtggc aggtgagaat
     3901 gtcggtaatt taacgtgacc agtcgtaggc acgacattga acattgaata ggccggtgtg
     3961 aaatgggtgt taagtcttaa tataaagttg tgttctggct aatctctctt taacagccat
     4021 aaatgtgatt tttaactaca gttttgaggg tgctggtgag catgttgcac acttgatgga
     4081 tagccggcat gggaagttct ttgtgcaggc agtgctgcag cagggtgtga cctactttag
     4141 ctagccggct aaccagctct catctgatgt aacgtaaacc ccatcaatct aacatcggct
     4201 cattcgtgct ttataagatc gaagatcttg ttattagtaa cgggtattgg catttagttt
     4261 taacttggtt aattttcttt ttgttatgtg ttctatttaa atgtagcctg cagccccctc
     4321 tgtctttaat gaagctgaag attaacgtta acgttagtga agtgttgtat ccgctatgaa
     4381 agatttgaat tttagaggtg tggtccactt taataacggc tgcggagttc tctggctgca
     4441 cttcagccgc tgtgactgtc gaagcagagc ccatcagttt ttcaggatct cggtgatttt
     4501 tgaggctaga ctatgaactg aaccgactga gagtgaccgt ccagaaatgc tttagtgtga
     4561 agtctccgct cacgaggagt ccgtggactg actgcagatc tgtgacgcag taacacccgg
     4621 cggcagacac ccgttgaaat gcggttgtgt aattgatcgc aggcgagggt ggaagaggat
     4681 gtgaaacttc atttgtgtaa aatttaggga gtggtcctgg tgtgatgaat gtcgaaatct
     4741 gttccttttt actgagccct acgactctgg ctgagtgcca caccgccggc agccgcaaag
     4801 cggctcaaac cattgccttt tatggtaata atgagagaat gcagagggac ttcctttgtc
     4861 tggcatatct gaggcgcgcg ctgtcactag cgcccaccag cggtcagact gtagaatgca
     4921 gagcaaaaca ggaagttgac tccagatggt cacatgcttg ctgaaacgcc tctccctggc
     4981 agcagtgcaa ttctaacagt ttccctttct tttacagttc agcctcaagt ttgtacaaaa
     5041 aagcaggctc cgcggccgcc cccttcacca tgacagttgt ggctggggac aacatggatg
     5101 agacctctgc cgtgccgggg catccgcagg acacataccc ccctgaccat gacgatcatg
     5161 aatgctgtga acgggtggtc atcaatatag cgggcctgcg attcgagacg cagcttaaaa
     5221 ctctcgccca gttcccagag actctcttag gtaacccaaa gaagcggatg cgttatttcg
     5281 atcccttgag aaacgagtac ttctttgaca gaaatcgtcc gagctttgat gccatcctgt
     5341 actattatca gtccggcgga cgactgagaa ggccggcgaa tgtcccgttg gatatgttct
     5401 cagaagaaat taagttctat gaacttgggg tggaggcaat ggagaagttt cgtgaggatg
     5461 agggctttat ccgtgaggag gagcgacctt taccagagaa agagtttcag cgacaaatct
     5521 ggctcctgtt tgagcatcct gagagctcag ggccagccag aggaattgct attgtgtctg
     5581 ttatggtcat tttaatttcc atcgtcattt tctgtttgga gactttacct gaactgaaag
     5641 aggaccctga ggggcgtata aagattatag gcaacaccac atactactac aaacctagca
     5701 ttctggccga tccattcttc atcattgaga ccctctgcat tatctggttt tcattcgaat
     5761 tgatagtgcg cttctttgct tgcccaagca aagcggcctt ctttaagaac atgatgaaca
     5821 ccattgacat tgtggctatt atcccttact tcatcacgct gggcacagag ttagcagagg
     5881 aacaagacgg aaaagaggga atgggcgagc aggcaacatc actggccatc ctcagggtga
     5941 tccgtctggt cagggtgttc agaatcttca agctgtccag gcactccaaa ggccttcaga
     6001 ttttagggca gaccctcaaa gccagtatgc gagagctcgg attgcttatt ttcttcctct
     6061 tcattggcgt catattgttc tccagtgctg tgtattttgc agaagcagag gaaaaggagt
     6121 cctacttcag cagcatccca gatgcattct ggtgggcagt tgtgtcaatg actaccgtag
     6181 gctacgggga catggtccca gtgaccatcg ggggcaagat tgtgggttcc ctgtgcgcca
     6241 tcgctggtgt gctgaccatt gccctccccg tgccagtcat tgtgtccaac ttcaactatt
     6301 tctatcatag agaaaccgaa ggagaggagc aggcccaact cctgaacgtg agcaacccga
     6361 acattgcctc cgattcaaac tctagtcgcc gaagctcctc aaccgtcagc aagtctgagt
     6421 acatggagat agatggagac ataaacaaca gcatcgacaa tttcagagag gccaacctca
     6481 gaactggcaa ctgcactatc cccaatcaga actgtgtgaa caaaagtaag ctgctaaccg
     6541 atgtctagaa gggtgggcgc gccgacccag ctttcttgta caaagtggcc gcccctctcc
     6601 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     6661 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     6721 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     6781 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     6841 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     6901 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     6961 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     7021 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
     7081 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     7141 tttcctttga aaaacacgat gataatatgg ccacaaccat ggctccaaag aagaagcgta
     7201 aggtaaatcg atcgaattca aggcctctcg agcctctaga aggtggcgga atggtgagca
     7261 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7321 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7381 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7441 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7501 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7561 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7621 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7681 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7741 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7801 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7861 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7921 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ctgcagccct
     7981 atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa
     8041 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     8101 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     8161 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     8221 gtggtatggc tgattatgat cctctagatc agatctgcga agatacggcc acgggtgctc
     8281 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     8341 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     8401 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
     8461 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
     8521 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
     8581 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     8641 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     8701 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     8761 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
     8821 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     8881 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag gcattttggt
     9961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11041 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    11101 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    11161 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    11221 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc acgctggcta
    11281 agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat
    11341 gacggctact acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc
    11401 aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca
    11461 gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg
    11521 ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa
    11581 aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa
    11641 gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt
    11701 gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt
    11761 ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt
    11821 tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt
    11881 tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc
    11941 tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg
    12001 atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca
    12061 gagactccct ggtgtctgaa acacaggcca gatgggccct cgagcaggaa acagctatga
    12121 ccatgattac gccaagctat caactttgta tagaaaagtt gaaaattcca gtttgaagaa
    12181 acttttcaag actcaccttt tcactgttgg tcttaattta ggttgattat ttttgtcaat
    12241 ttaataatta cattgtattg ttcatatttt attgtcttat tgtggtgttt ttacagcttt
    12301 tttattgttc tgctttgtta ctgtttgcct tgtatgcatt gtagcgcttt gggtctgaga
    12361 aaagcgcact ataaataaac tgtattatta ttattaggga tggacatcac ggtggcgcga
    12421 tgggtggcac aattacctca cag
//
