LOCUS       sequence-325739-       12664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12664)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 12664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnk9-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5257..5281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6761..6785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6788..7362,7363..7365,7366..7374)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6955..6972)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7182..7201
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7381..7401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7447..7449,7450..7452,7453..8166)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7447..7449,7450..7452,7453..8163)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7492..7513)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7753..7772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8100..8121
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8173..8192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8174..8192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8197..8331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8394..8414)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8422..8438)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8454..8588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9027..9214,9215..9226)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9260..9276)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9260..9276)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9313..9333)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9313..9331)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9352..9368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9365..9387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9376..9392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9400..9406,9407..9424,9425..9430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9445..9466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9583..9600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9754..10342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9834..9853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10513..11304,11305..11373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11136..11155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11374..11478)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11641..11662)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(12011..12022,12023..12185)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     12558..12574
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12594..12614
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 cttaatttag gttgattatt tttgtcaatt taataattac attgtattgt tcatatttta
       61 ttgtcttatt gtggtgtttt tacagctttt ttattgttct gctttgttac tgtttgcctt
      121 gtatgcattg tagcgctttg ggtctgagaa aagcgcacta taaataaact gtattattat
      181 tattagggat ggacatcacg gtggcgcgat gggtggcaca attacctcac agcaagtagg
      241 ttgctgttta gagccccggc tggatcagtt ggcagttctg tgtggagttt gcatgttctc
      301 cctgttcaag taggtttcct cccggtgctc tggtttcccc tcacaagtcc aaagacatga
      361 tgtataggtg aatggggtaa gctaaattga ccatagtgag tgtgtgtgtg tgtgtgaatg
      421 agagtgtata ggtgtttccc agtgttaggt tgcaactgga aggacatctg ctgtgtaaaa
      481 catatgcagg ataagttggc ggttcattcc cctatggtga cccctggtta attaaaggac
      541 taagttgaaa atgaatgaat gaatgaatga ttagagataa accgatacca cttttttaca
      601 aaccgatatg agtacaagta taatagtttc tgttactcga caatactgag taccgattct
      661 gatacttctt ggatttggtt tcaatgtaat taatttgaaa gaaggtttta tttcttgcct
      721 gtcgcaagga tgaacacaaa aatctttacc agaaggatat tttaagctaa gaatatacgc
      781 acgttgttga caaccctgta tcgattaccg attaaacgtg gacatttaaa ggataagtta
      841 tgcttgcgcc atcacaactt tacacattac atgatcaaag atcatattga atattcggtt
      901 tattcgtcag aagcctatag atttgttaaa agtgtatttt ataaacggat ggtttataaa
      961 gactaagcta ttaatatatc agacattcat taatacatca agacatcgat gtcaggtagg
     1021 acaacaaatc acatccatgt aatgaaatct tgttgagtgg tattggtgcg tggtttcagc
     1081 atttcatgtg caatacgagt aagaatattt taaccaagta tcgacccaaa acctgatact
     1141 ggtatcggtg catcccttat tattattatt atcaaaaaca cacttgtttt atttatgtgg
     1201 gtgccaaaca acaaactatg aaatgcagca tgacacgccc aaaaagtgtt cctttgagaa
     1261 caacttcata aatatgtttc ttatcattat gcatgaatgg tgtactggga gagctttggt
     1321 atcagaattg agataagaga ctttggcatt ctaaaggaca atgacattat cacactgaca
     1381 gtcaacaaca ctttattgct ctggaaatct tatgtcatgg ggcaaagctg aggttcatta
     1441 ggacagtacc ttatttagag atgaatggga aggctgtagt gggctgctat cagggaaggg
     1501 tggtggtcct cgggggaact atggactcac aaaagcacat ctgctgtaac aagatcccct
     1561 gtgcaatcta caaaagttac aaagagaagc atggttggtt aaaaaataga attgactgga
     1621 tatgctgaat gcattgtttg ttgtgtgtta catcacatta accacagatt ggtgctaaat
     1681 atttgcttat cttataaaaa cctgtgtgtt tataaagggg ggtgtggatg gatgcttggc
     1741 attctttgaa aagatgttgg gggatggaag gaggagacct tattgatgag tatccagttt
     1801 gcagataaca agtttttaga cattcatact atgatgctgt ttatgtctgt tttttctcat
     1861 tttttctata cacaaacagc tataaaacat tcacttttat atctgatgct gtatattaaa
     1921 tttcgttgta tttattctgt ttgctctgtt gggtcatagc tagggccaga cagaatctgc
     1981 tgacattttt tgctttttct gcagagaatt ttgtaaacaa tctgtgattt atgcgagatt
     2041 ttttcgggag tatcgtaact aaaaacttac tatatgaaat aaaaaaaaat aatacctttt
     2101 taacttttaa taaatatata caatgcaagt ccacctagat ccaattattt ggtcgacaaa
     2161 gcaagtctct cacataatat atcttctaaa agacagaaaa cattactttc agaccatatt
     2221 gtaatcaaat gaacactttc atattagtca ataatattac ggaaatgtat ttaaaaactg
     2281 aataaacata tatttacacc tttgtcttta tttctagagt gcttttacaa tgtagattgt
     2341 gtcaaagcag cttaacttag aggattagta tagtaaattg aaactgtgtc agtccatttt
     2401 ttaagagttt aagttcagtt cagtttagtt cagtgtgagt taatttttac tgctgagagt
     2461 ccaaacacta aagagcaaat ccaaatacac aagtcaataa ataaactcaa tgatgggcta
     2521 aagatctggg gaaatctgca gatttctgtg cgcacagatt ctgtgtgggc ctagtcatta
     2581 cctatactca acgtttactc tgagaaactt ccatatttta gaggtaaaat gctcagaatt
     2641 gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag tcacatcacc
     2701 tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg tttataactc
     2761 ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca tgtagtttat
     2821 catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa tatctgaaat
     2881 gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt tttttttcta
     2941 cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa aaagaaatgt
     3001 tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt taagatcaag
     3061 acttgacata taacatttct aatgaacatt aaacctgagg taggcccact tgcaaaaatg
     3121 taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa aatagtgaag
     3181 caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact ttcttaaagt
     3241 gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa gtagtgtcat
     3301 cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat tagatccccc
     3361 attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag agctgctgtt
     3421 gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg acgcattctg
     3481 ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc gttgtcgcag
     3541 aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt tataggaaat
     3601 cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc aagttgcaag
     3661 aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc aatcagagca
     3721 cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc atataaaaga
     3781 gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt gcggaatatc
     3841 atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt gagttggttt
     3901 tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa aatgtgtaaa
     3961 tggataaata tgatttgatg aagtctttag acagttcata acggtctctt tgtagttatc
     4021 tactaggttt gatttataag tggtggttct tttgataatc tatgttacat ttaataaata
     4081 ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga atgtcggtaa
     4141 tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg tgaaatgggt
     4201 gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc ataaatgtga
     4261 tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg gatagccggc
     4321 atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt agctagccgg
     4381 ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg ctcattcgtg
     4441 ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt tttaacttgg
     4501 ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc tctgtcttta
     4561 atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg aaagatttga
     4621 attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg cacttcagcc
     4681 gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt tttgaggcta
     4741 gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt gaagtctccg
     4801 ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc ggcggcagac
     4861 acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg atgtgaaact
     4921 tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat ctgttccttt
     4981 ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa agcggctcaa
     5041 accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg tctggcatat
     5101 ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg cagagcaaaa
     5161 caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg gcagcagtgc
     5221 aattctaaca gtttcccttt cttttacagt tcagcctcaa gtttgtacaa aaaagcaggc
     5281 ttcaccatgg cactgcgcgc cgggaggact ttgctgtggt tcgggcaggt tttgcgcaga
     5341 gtttcgcggc tgcagggctt gtggtcccgg cgctccagca gtctgctgtg tctctccaac
     5401 agccaggagc acgaccaagg gacctgttac agccgcaacc ggtgtccgaa gacgcatcgg
     5461 cagcaacact gcccctatcc gccctttcca gactgctgct gcgccttccc gggggataag
     5521 aggggacatg aacccctcag ccgccggttc ctggtggcca tgaagaggca gaacgtgcgg
     5581 acgctgtccc tgatcgtctg cacgttcacc tatctgctgg tcggggccgc ggtgtttgac
     5641 gcgctcgagt cggacttcga gatgcgggag aaggagcagc tggaggcgga ggagaagcgc
     5701 ctccagggca agtacaacat cagtgaggat gactataaaa agctgcagac catcatcatg
     5761 gaggccgagc cgcacagagc aggggtccag tggaaatttg caggctcgtt ttactttgcc
     5821 atcactgtca taaccactat aggttatggt cacgcagctc caggcacaga tgctgggaag
     5881 gccttctgca tgttttacgc cgtgctgggg atcccgctca cgctggtgat gttccagagc
     5941 ctcggcgagc ggatgaacac cttcgtcaag tacttgctaa agcgcatcaa gaagtgctgc
     6001 gggatgcgca tcacagaggt gtccatggag aacatggtaa cagtgggctt cttctcttgc
     6061 atgggcacgc tctgcatcgg cgctgccgca ttttcccagt acgaggactg gagcttcttc
     6121 cagtcctatt actattgttt cataacactc accactattg gcttcgggga cttcgtggcc
     6181 ctccagaaga acaaagccct gcagaaaaag cctctttacg tggcgttcag cttcatgtac
     6241 atcctagtgg ggctgacggt catcggggcc ttcctcaatc tggtggtgct acgcttcttg
     6301 accatgaaca gcgaggacga gcgacgggat gctgaggaga gggcgtccct ggcggggaac
     6361 cgcagtagca tgatcatcca catccaagaa gacacgcttc agaggagtcg gcggaggcga
     6421 gaacagcagc agaaccgcta ccgtcccgaa gtcaccgatc tccagtcggt ttgctcctgc
     6481 atgcactacc actcgcatga gttcgggagc tcggtcggcc tcgggttggg attgggagga
     6541 ggtggcggag gtgcgtttcc gcagcagaac tcattcggct cgcagttgag cccgcatccg
     6601 taccatcact accactcaac ggtgtcctac cgcatcgagg agatctcacc gagcaccttg
     6661 aaaaacagct tccttccttc cccgatcagc tccatctctc ctgggctgca cagcttcgcg
     6721 gaaaacctcc ggcttatgag acgacgcaag tccatctgag acccagcttt cttgtacaaa
     6781 gtggccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6841 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6901 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6961 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     7021 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     7081 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     7141 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     7201 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     7261 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     7321 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggct
     7381 ccaaagaaga agcgtaaggt aaatcgatcg aattcaaggc ctctcgagcc tctagaaggt
     7441 ggcggaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     7501 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     7561 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     7621 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     7681 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     7741 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     7801 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     7861 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     7921 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     7981 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     8041 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     8101 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     8161 aagtaactgc agccctatag tgagtcgtat tacgtagatc cagacatgat aagatacatt
     8221 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     8281 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     8341 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttccaacttt
     8401 attatacata gttgataatt cactggccgt cgttttacgg taccatcgat gatgatccag
     8461 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     8521 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     8581 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     8641 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     8701 atcagatctg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt
     8761 gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt
     8821 tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga
     8881 cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt gaaaacgatt
     8941 ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat tcaattggtt
     9001 tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac tttttgactg
     9061 taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa
     9121 agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact
     9181 taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct
     9241 accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc ggtggagctc
     9301 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     9361 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9421 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9481 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9541 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10381 gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10621 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11341 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11401 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11461 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
    11521 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
    11581 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
    11641 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    11701 accgtctatc agggcgatgg cccacgctgg ctaagaactc atcagcctcc ccggtccatc
    11761 tacccacgta ccaatgcacc aattggccac aatgacggct actacatggt gccattcctt
    11821 cctctttata ggaatggaga ctacctcctg tccaacaatg ctcttggata cgagtacgcc
    11881 tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg
    11941 tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag gtttgtccag
    12001 gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta
    12061 cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct tacttgagta
    12121 aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc
    12181 aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat taaatgaaat
    12241 gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa ctataagaca
    12301 gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt aacttcagta
    12361 gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg aaaagcaatc
    12421 ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta ttagtcttga
    12481 tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct gaaacacagg
    12541 ccagatgggc cctcgagcag gaaacagcta tgaccatgat tacgccaagc tatcaacttt
    12601 gtatagaaaa gttgaaaatt ccagtttgaa gaaacttttc aagactcacc ttttcactgt
    12661 tggt
//
