LOCUS       sequence-325743-       12310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 12310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(91..111)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(119..135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    151..285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(724..911,912..923)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(957..973)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(957..973)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1010..1030)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1010..1028)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1049..1065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1062..1084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1073..1089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1097..1103,1104..1121,1122..1127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1142..1163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1280..1297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1451..2039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1531..1550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2210..3001,3002..3070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2833..2852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3071..3175)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3338..3359)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(3708..3719,3720..3882)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     4255..4271
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4291..4311
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    9618..9642
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10768..10792)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(10795..11369,11370..11372,11373..11381)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10962..10979)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11189..11208
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11388..11408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(11454..11456,11457..11459,11460..12173)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11454..11456,11457..11459,11460..12170)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11499..11520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11760..11779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12107..12128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(12180..12199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12181..12199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    join(12204..12310,1..28)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
       61 ggttcagggg gaggtgtggg aggttttttc caactttatt atacatagtt gataattcac
      121 tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata cattgatgag
      181 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      241 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      301 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      361 ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga agatacggcc
      421 acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat
      481 cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt
      541 caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga
      601 acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat
      661 ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga
      721 atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa
      781 aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt
      841 gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat
      901 tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc
      961 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     1021 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1081 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1141 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1201 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1261 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1861 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2101 gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2161 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2341 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2521 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3061 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3181 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3241 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3301 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3361 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3421 acgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat
     3481 tggccacaat gacggctact acatggtgcc attccttcct ctttatagga atggagacta
     3541 cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg
     3601 cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat
     3661 atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa
     3721 agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt
     3781 aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa
     3841 aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt
     3901 gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt
     3961 tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc
     4021 tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta
     4081 gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca
     4141 caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga
     4201 aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccct cgagcaggaa
     4261 acagctatga ccatgattac gccaagctat caactttgta tagaaaagtt gaaaattcca
     4321 gtttgaagaa acttttcaag actcaccttt tcactgttgg tcttaattta ggttgattat
     4381 ttttgtcaat ttaataatta cattgtattg ttcatatttt attgtcttat tgtggtgttt
     4441 ttacagcttt tttattgttc tgctttgtta ctgtttgcct tgtatgcatt gtagcgcttt
     4501 gggtctgaga aaagcgcact ataaataaac tgtattatta ttattaggga tggacatcac
     4561 ggtggcgcga tgggtggcac aattacctca cagcaagtag gttgctgttt agagccccgg
     4621 ctggatcagt tggcagttct gtgtggagtt tgcatgttct ccctgttcaa gtaggtttcc
     4681 tcccggtgct ctggtttccc ctcacaagtc caaagacatg atgtataggt gaatggggta
     4741 agctaaattg accatagtga gtgtgtgtgt gtgtgtgaat gagagtgtat aggtgtttcc
     4801 cagtgttagg ttgcaactgg aaggacatct gctgtgtaaa acatatgcag gataagttgg
     4861 cggttcattc ccctatggtg acccctggtt aattaaagga ctaagttgaa aatgaatgaa
     4921 tgaatgaatg attagagata aaccgatacc acttttttac aaaccgatat gagtacaagt
     4981 ataatagttt ctgttactcg acaatactga gtaccgattc tgatacttct tggatttggt
     5041 ttcaatgtaa ttaatttgaa agaaggtttt atttcttgcc tgtcgcaagg atgaacacaa
     5101 aaatctttac cagaaggata ttttaagcta agaatatacg cacgttgttg acaaccctgt
     5161 atcgattacc gattaaacgt ggacatttaa aggataagtt atgcttgcgc catcacaact
     5221 ttacacatta catgatcaaa gatcatattg aatattcggt ttattcgtca gaagcctata
     5281 gatttgttaa aagtgtattt tataaacgga tggtttataa agactaagct attaatatat
     5341 cagacattca ttaatacatc aagacatcga tgtcaggtag gacaacaaat cacatccatg
     5401 taatgaaatc ttgttgagtg gtattggtgc gtggtttcag catttcatgt gcaatacgag
     5461 taagaatatt ttaaccaagt atcgacccaa aacctgatac tggtatcggt gcatccctta
     5521 ttattattat tatcaaaaac acacttgttt tatttatgtg ggtgccaaac aacaaactat
     5581 gaaatgcagc atgacacgcc caaaaagtgt tcctttgaga acaacttcat aaatatgttt
     5641 cttatcatta tgcatgaatg gtgtactggg agagctttgg tatcagaatt gagataagag
     5701 actttggcat tctaaaggac aatgacatta tcacactgac agtcaacaac actttattgc
     5761 tctggaaatc ttatgtcatg gggcaaagct gaggttcatt aggacagtac cttatttaga
     5821 gatgaatggg aaggctgtag tgggctgcta tcagggaagg gtggtggtcc tcgggggaac
     5881 tatggactca caaaagcaca tctgctgtaa caagatcccc tgtgcaatct acaaaagtta
     5941 caaagagaag catggttggt taaaaaatag aattgactgg atatgctgaa tgcattgttt
     6001 gttgtgtgtt acatcacatt aaccacagat tggtgctaaa tatttgctta tcttataaaa
     6061 acctgtgtgt ttataaaggg gggtgtggat ggatgcttgg cattctttga aaagatgttg
     6121 ggggatggaa ggaggagacc ttattgatga gtatccagtt tgcagataac aagtttttag
     6181 acattcatac tatgatgctg tttatgtctg ttttttctca ttttttctat acacaaacag
     6241 ctataaaaca ttcactttta tatctgatgc tgtatattaa atttcgttgt atttattctg
     6301 tttgctctgt tgggtcatag ctagggccag acagaatctg ctgacatttt ttgctttttc
     6361 tgcagagaat tttgtaaaca atctgtgatt tatgcgagat tttttcggga gtatcgtaac
     6421 taaaaactta ctatatgaaa taaaaaaaaa taataccttt ttaactttta ataaatatat
     6481 acaatgcaag tccacctaga tccaattatt tggtcgacaa agcaagtctc tcacataata
     6541 tatcttctaa aagacagaaa acattacttt cagaccatat tgtaatcaaa tgaacacttt
     6601 catattagtc aataatatta cggaaatgta tttaaaaact gaataaacat atatttacac
     6661 ctttgtcttt atttctagag tgcttttaca atgtagattg tgtcaaagca gcttaactta
     6721 gaggattagt atagtaaatt gaaactgtgt cagtccattt tttaagagtt taagttcagt
     6781 tcagtttagt tcagtgtgag ttaattttta ctgctgagag tccaaacact aaagagcaaa
     6841 tccaaataca caagtcaata aataaactca atgatgggct aaagatctgg ggaaatctgc
     6901 agatttctgt gcgcacagat tctgtgtggg cctagtcatt acctatactc aacgtttact
     6961 ctgagaaact tccatatttt agaggtaaaa tgctcagaat tgtaaaaagt cttgtcaaaa
     7021 tacttggaat ttgccaaaaa caaaataaaa gtcacatcac ctcattgtcc ttggtatcag
     7081 caagcacttt taacacaatc agctgttttt gtttataact cccactgaag gccatgtgca
     7141 ttactcccaa gttacacagt aaaaagtcac atgtagttta tcatgatcac tgctgctcaa
     7201 gcacagcttt atctgttatt cagtgtggca atatctgaaa tgtgtgcaaa atattattta
     7261 ttgtagctga tcaccaacac tttcagtgtt ttttttttct acttttaatg agaattatgt
     7321 tcttaggatt tatccttata aacctcttta aaaagaaatg ttatgtctgc atagacacga
     7381 catgaaatta tgaacagaaa atttaactat ttaagatcaa gacttgacat ataacatttc
     7441 taatgaacat taaacctgag gtaggcccac ttgcaaaaat gtaactgcta tcactcactt
     7501 tatacatcat tgtgtaactt gtacacacaa aaatagtgaa gcaatgtggc atggaatgca
     7561 ggtcccattc attcaaagtt attaaagcac tttcttaaag tgcattacta ctgagctaag
     7621 accagctagt tgctcataca acatactaca agtagtgtca tcctaatttg gataaaaaaa
     7681 atgtccattt tatacatgga aaagtactca ttagatcccc cattgtttgt attacggtat
     7741 ttcgtgaaca caagaggtaa atgacctaca gagctgctgt tgtgttagat tgaaaacaca
     7801 acacaggatc atggaggcat ccatcattat gacgcattct gccacttgta actctgcaca
     7861 gactttttga aaagtttaat tgagtcttgg cgttgtcgca gaaaaatacg aaaaattctg
     7921 tgttaaaatt tgcaaataaa aattccagtt ttataggaaa tcgccatggc cttgttcttt
     7981 aaccagttaa gcttcccctt ctttcactct caagttgcaa gaagcaagtg tagcaatgtg
     8041 cacgcgacag ccgggtgtgt gacgctggac caatcagagc acagagctcc gaaagtttac
     8101 cttttatggc tagagccggg tatgtgccgt catataaaag agcgcgccca gcttttcagc
     8161 ctcactttga gctcctccac acgcagctag tgcggaatat catctgcttg taacccattc
     8221 tcttaagtcg acaacccccc aaacccaagg tgagttggtt ttacagtttt taagcgttta
     8281 attatagttt ctatctatta atgttaagta aaatgtgtaa atggataaat atgatttgat
     8341 gaagtcttta gacagttcat aacggtctct ttgtagttat ctactaggtt tgatttataa
     8401 gtggtggttc ttttgataat ctatgttaca tttaataaat attggctgta tcttaacttt
     8461 tttgtagcgt tagtggatgt ggcaggtgag aatgtcggta atttaacgtg accagtcgta
     8521 ggcacgacat tgaacattga ataggccggt gtgaaatggg tgttaagtct taatataaag
     8581 ttgtgttctg gctaatctct ctttaacagc cataaatgtg atttttaact acagttttga
     8641 gggtgctggt gagcatgttg cacacttgat ggatagccgg catgggaagt tctttgtgca
     8701 ggcagtgctg cagcagggtg tgacctactt tagctagccg gctaaccagc tctcatctga
     8761 tgtaacgtaa accccatcaa tctaacatcg gctcattcgt gctttataag atcgaagatc
     8821 ttgttattag taacgggtat tggcatttag ttttaacttg gttaattttc tttttgttat
     8881 gtgttctatt taaatgtagc ctgcagcccc ctctgtcttt aatgaagctg aagattaacg
     8941 ttaacgttag tgaagtgttg tatccgctat gaaagatttg aattttagag gtgtggtcca
     9001 ctttaataac ggctgcggag ttctctggct gcacttcagc cgctgtgact gtcgaagcag
     9061 agcccatcag tttttcagga tctcggtgat ttttgaggct agactatgaa ctgaaccgac
     9121 tgagagtgac cgtccagaaa tgctttagtg tgaagtctcc gctcacgagg agtccgtgga
     9181 ctgactgcag atctgtgacg cagtaacacc cggcggcaga cacccgttga aatgcggttg
     9241 tgtaattgat cgcaggcgag ggtggaagag gatgtgaaac ttcatttgtg taaaatttag
     9301 ggagtggtcc tggtgtgatg aatgtcgaaa tctgttcctt tttactgagc cctacgactc
     9361 tggctgagtg ccacaccgcc ggcagccgca aagcggctca aaccattgcc ttttatggta
     9421 ataatgagag aatgcagagg gacttccttt gtctggcata tctgaggcgc gcgctgtcac
     9481 tagcgcccac cagcggtcag actgtagaat gcagagcaaa acaggaagtt gactccagat
     9541 ggtcacatgc ttgctgaaac gcctctccct ggcagcagtg caattctaac agtttccctt
     9601 tcttttacag ttcagcctca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
     9661 ccatgcctac caccatgaca aacaccaccg ctgaccaaaa ggcctcctgc cctttgatgg
     9721 tcaaacctca gcgtcgccgc ctggtcagca aagacggtcg gagccagaca agaggaaaca
     9781 caagaggagg ctccagagag acgtgtttca gtgctctgcg ggacttgtgg ggcacatggc
     9841 tggcgctacg gtggcggtgg gtggttttgg cattttgtgg ctcattcctg cttcactggc
     9901 tgctgtttgc tgtattatgg tacctgctgg ctcgggtcaa tggagacctg gatgtcctgg
     9961 atcatgattc tcctcctccc ggtcatgtgc tgtgtgttaa acatgtgaat gggttcacag
    10021 ccgctttttc attcgccctg gagacgcaac tcactattgg gtatggcacc atgtacccaa
    10081 atgcagactg tcccactgcc atcgcgctgc ttgccctgca gatgctgctg ggactcatgt
    10141 tggaggcctt tatcactggt gcatttgtag ccaagttctc tcggcctcag aagcgatgtg
    10201 acgggatcct attcagccct caggctgtgg tgtgtgagca gaaaggccag cgctgtctaa
    10261 tgtttcgagt gtgtaacctc cagccacagc cgctagtgga cgtatccgtg agtgctgtcc
    10321 tctacgagga gcgagacgat cacgaactcc accaaactgc tctggagttc agcatcgaca
    10381 acctgggctc cagatcttgc cctcttttcc tctcccctct gaccttcttt caccctttaa
    10441 atccatccac tcctttcatc aacaacccaa gcagccaaac acactttgag cttgtagtgt
    10501 ttctcactgc gactcaggaa agcacagggt ctggttacca caagaggacg tcttacctgc
    10561 ctgacgagat ccaatatggc tactgcttct ccaaggtgac gtctgtacac cagaacaaaa
    10621 ccccaaacat gcgctatttt gacacggtgc cctgtcccct cacactagcc aacacacaca
    10681 ccactgatac accggataaa gagcatgtgg tggttcagct gaatggagaa ggcagcgacc
    10741 gcgtggagta gaagggtggg cgcgccgacc cagctttctt gtacaaagtg gccgcccctc
    10801 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
    10861 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
    10921 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
    10981 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
    11041 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
    11101 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
    11161 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
    11221 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
    11281 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
    11341 ggttttcctt tgaaaaacac gatgataata tggccacaac catggctcca aagaagaagc
    11401 gtaaggtaaa tcgatcgaat tcaaggcctc tcgagcctct agaaggtggc ggaatggtga
    11461 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
    11521 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
    11581 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
    11641 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
    11701 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
    11761 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
    11821 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
    11881 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
    11941 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
    12001 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
    12061 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
    12121 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taactgcagc
    12181 cctatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttggac
    12241 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
    12301 ctttatttgt
//
