LOCUS       sequence-32576-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN24-R4R3
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     766..788
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 actgcgtgca tattttacct ggaaataaat tattttatga tgaaagttaa atacaataag
       61 atacaacggg aaacgtaaga aaaattcaat aaaatcaatt gtacacgtag gttttcaagc
      121 aaatttaaga atacaatttt acaaagtcct aaatgtcatt tttcctgctc aaacttttga
      181 ctgaaatgca taaaaattcg ctgataaaaa caagcagaat acgccaaaat agttgcaaat
      241 tataaaattt acagaattaa tacaaaaaat tcgatatttt actcaaaaaa atggaaaaaa
      301 agaagaaaaa taaattaaac tttcgttgag ctggcctaaa atctaatagc aagcgcgctc
      361 cattgccaaa cggtgtaaac gcggagcctc gtttgcgtgt agtttttatc gtaaatcgac
      421 acaacttctt tcaaaataaa ttatttctta tgagtaattt tctgcattct attgttgtgt
      481 ttcaattaat cttattttta atttgatttt attcaaaatt atataatttt attaagatgc
      541 tgggttcttc tctccgcatt ttctcccctc gagggggggc ccggcatatg cggtgtgaaa
      601 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
      661 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      721 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      781 gtaaaacgac gg
//
