LOCUS       sequence-325771-       12349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj1b-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5257..5281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6446..6470)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6473..7047,7048..7050,7051..7059)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6640..6657)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6867..6886
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7066..7086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7132..7134,7135..7137,7138..7851)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7132..7134,7135..7137,7138..7848)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7177..7198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7438..7457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7785..7806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7858..7877)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7859..7877)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7882..8016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8079..8099)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8107..8123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8139..8273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8712..8899,8900..8911)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8945..8961)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8945..8961)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8998..9018)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8998..9016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9037..9053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9050..9072)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9061..9077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9085..9091,9092..9109,9110..9115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9130..9151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9268..9285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9439..10027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9519..9538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10198..10989,10990..11058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10821..10840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11059..11163)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11326..11347)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(11696..11707,11708..11870)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     12243..12259
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12279..12299
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 cttaatttag gttgattatt tttgtcaatt taataattac attgtattgt tcatatttta
       61 ttgtcttatt gtggtgtttt tacagctttt ttattgttct gctttgttac tgtttgcctt
      121 gtatgcattg tagcgctttg ggtctgagaa aagcgcacta taaataaact gtattattat
      181 tattagggat ggacatcacg gtggcgcgat gggtggcaca attacctcac agcaagtagg
      241 ttgctgttta gagccccggc tggatcagtt ggcagttctg tgtggagttt gcatgttctc
      301 cctgttcaag taggtttcct cccggtgctc tggtttcccc tcacaagtcc aaagacatga
      361 tgtataggtg aatggggtaa gctaaattga ccatagtgag tgtgtgtgtg tgtgtgaatg
      421 agagtgtata ggtgtttccc agtgttaggt tgcaactgga aggacatctg ctgtgtaaaa
      481 catatgcagg ataagttggc ggttcattcc cctatggtga cccctggtta attaaaggac
      541 taagttgaaa atgaatgaat gaatgaatga ttagagataa accgatacca cttttttaca
      601 aaccgatatg agtacaagta taatagtttc tgttactcga caatactgag taccgattct
      661 gatacttctt ggatttggtt tcaatgtaat taatttgaaa gaaggtttta tttcttgcct
      721 gtcgcaagga tgaacacaaa aatctttacc agaaggatat tttaagctaa gaatatacgc
      781 acgttgttga caaccctgta tcgattaccg attaaacgtg gacatttaaa ggataagtta
      841 tgcttgcgcc atcacaactt tacacattac atgatcaaag atcatattga atattcggtt
      901 tattcgtcag aagcctatag atttgttaaa agtgtatttt ataaacggat ggtttataaa
      961 gactaagcta ttaatatatc agacattcat taatacatca agacatcgat gtcaggtagg
     1021 acaacaaatc acatccatgt aatgaaatct tgttgagtgg tattggtgcg tggtttcagc
     1081 atttcatgtg caatacgagt aagaatattt taaccaagta tcgacccaaa acctgatact
     1141 ggtatcggtg catcccttat tattattatt atcaaaaaca cacttgtttt atttatgtgg
     1201 gtgccaaaca acaaactatg aaatgcagca tgacacgccc aaaaagtgtt cctttgagaa
     1261 caacttcata aatatgtttc ttatcattat gcatgaatgg tgtactggga gagctttggt
     1321 atcagaattg agataagaga ctttggcatt ctaaaggaca atgacattat cacactgaca
     1381 gtcaacaaca ctttattgct ctggaaatct tatgtcatgg ggcaaagctg aggttcatta
     1441 ggacagtacc ttatttagag atgaatggga aggctgtagt gggctgctat cagggaaggg
     1501 tggtggtcct cgggggaact atggactcac aaaagcacat ctgctgtaac aagatcccct
     1561 gtgcaatcta caaaagttac aaagagaagc atggttggtt aaaaaataga attgactgga
     1621 tatgctgaat gcattgtttg ttgtgtgtta catcacatta accacagatt ggtgctaaat
     1681 atttgcttat cttataaaaa cctgtgtgtt tataaagggg ggtgtggatg gatgcttggc
     1741 attctttgaa aagatgttgg gggatggaag gaggagacct tattgatgag tatccagttt
     1801 gcagataaca agtttttaga cattcatact atgatgctgt ttatgtctgt tttttctcat
     1861 tttttctata cacaaacagc tataaaacat tcacttttat atctgatgct gtatattaaa
     1921 tttcgttgta tttattctgt ttgctctgtt gggtcatagc tagggccaga cagaatctgc
     1981 tgacattttt tgctttttct gcagagaatt ttgtaaacaa tctgtgattt atgcgagatt
     2041 ttttcgggag tatcgtaact aaaaacttac tatatgaaat aaaaaaaaat aatacctttt
     2101 taacttttaa taaatatata caatgcaagt ccacctagat ccaattattt ggtcgacaaa
     2161 gcaagtctct cacataatat atcttctaaa agacagaaaa cattactttc agaccatatt
     2221 gtaatcaaat gaacactttc atattagtca ataatattac ggaaatgtat ttaaaaactg
     2281 aataaacata tatttacacc tttgtcttta tttctagagt gcttttacaa tgtagattgt
     2341 gtcaaagcag cttaacttag aggattagta tagtaaattg aaactgtgtc agtccatttt
     2401 ttaagagttt aagttcagtt cagtttagtt cagtgtgagt taatttttac tgctgagagt
     2461 ccaaacacta aagagcaaat ccaaatacac aagtcaataa ataaactcaa tgatgggcta
     2521 aagatctggg gaaatctgca gatttctgtg cgcacagatt ctgtgtgggc ctagtcatta
     2581 cctatactca acgtttactc tgagaaactt ccatatttta gaggtaaaat gctcagaatt
     2641 gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag tcacatcacc
     2701 tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg tttataactc
     2761 ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca tgtagtttat
     2821 catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa tatctgaaat
     2881 gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt tttttttcta
     2941 cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa aaagaaatgt
     3001 tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt taagatcaag
     3061 acttgacata taacatttct aatgaacatt aaacctgagg taggcccact tgcaaaaatg
     3121 taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa aatagtgaag
     3181 caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact ttcttaaagt
     3241 gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa gtagtgtcat
     3301 cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat tagatccccc
     3361 attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag agctgctgtt
     3421 gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg acgcattctg
     3481 ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc gttgtcgcag
     3541 aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt tataggaaat
     3601 cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc aagttgcaag
     3661 aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc aatcagagca
     3721 cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc atataaaaga
     3781 gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt gcggaatatc
     3841 atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt gagttggttt
     3901 tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa aatgtgtaaa
     3961 tggataaata tgatttgatg aagtctttag acagttcata acggtctctt tgtagttatc
     4021 tactaggttt gatttataag tggtggttct tttgataatc tatgttacat ttaataaata
     4081 ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga atgtcggtaa
     4141 tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg tgaaatgggt
     4201 gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc ataaatgtga
     4261 tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg gatagccggc
     4321 atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt agctagccgg
     4381 ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg ctcattcgtg
     4441 ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt tttaacttgg
     4501 ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc tctgtcttta
     4561 atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg aaagatttga
     4621 attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg cacttcagcc
     4681 gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt tttgaggcta
     4741 gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt gaagtctccg
     4801 ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc ggcggcagac
     4861 acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg atgtgaaact
     4921 tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat ctgttccttt
     4981 ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa agcggctcaa
     5041 accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg tctggcatat
     5101 ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg cagagcaaaa
     5161 caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg gcagcagtgc
     5221 aattctaaca gtttcccttt cttttacagt tcagcctcaa gtttgtacaa aaaagcaggc
     5281 tccgcggccg cccccttcac catgttccag ttcatccgga aacagcttca caatcacctg
     5341 gtggagcgca aaatcaggag aactcgcctc gtcaccaaag acggccactg caacatcaaa
     5401 ttcggcacca ccgaatatca aagccacttc gctttcctta aggacttctg gactacattc
     5461 gtcgaaatcc actggtgttt cgtaatcctt ttatttgccg cagccttcac cggcagctgg
     5521 ttcgttttcg gcctgctctg gtattcgcta gcgaaaagca acggagacct gtctgagtct
     5581 cagcagacga aatgcatcga gaacattaac agcctcacga ctgctttctt gtattcttta
     5641 gaaacccaaa ccactattgg atatggcggt cgggctctta cggggcactg tcctggcacc
     5701 gtcgctctat taataatcca atccctcatg ggcgccatca tcaactgctt catgtgtgga
     5761 ctcatcctgg ccaagatctc gctcccgaag agaagagcaa agactgtagc gttcagcgaa
     5821 acagccgtca tccgtctctg gaaggggaat ttatgcctgc agatacgagt tgccaatcta
     5881 cggaaaaccc tactgattgg aagccacatt tacggaaagc ttctgcggac tactatcact
     5941 cccgaaggtg aaactatcat tctggatcaa gtaaatgttg acttttcggt ggacgccggg
     6001 aaggataact tgttttttgt gtgtccgctt actatttatc atgtgatcga caaatccagt
     6061 ccgttcgcgg acatggcagc ggataacctt cagctccagg actttgagtt ggtggttttt
     6121 cttgacggca cggctgaatc tacaagcttg tcctgccaag tgcgaacttc ttaccttccc
     6181 agagagatca aatggggtta cgtgtttctc cccgtcatct ctcgcaccaa aactggaaaa
     6241 taccgtatcg atttatccaa tttttccaaa acgcagcaag cagatactcc acattgtgct
     6301 tgctgttttc acaacaaaca taacaagggg ctcattctcc accggcatgg aagcgaaggc
     6361 atcgataacc tgggatttga cgttattgac cttcaggact cggctgatgt aactgagaag
     6421 ggcgattaaa agggtgggcg cgccgaccca gctttcttgt acaaagtggc cgcccctctc
     6481 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6541 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6601 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6661 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6721 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6781 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6841 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6901 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     6961 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     7021 ttttcctttg aaaaacacga tgataatatg gccacaacca tggctccaaa gaagaagcgt
     7081 aaggtaaatc gatcgaattc aaggcctctc gagcctctag aaggtggcgg aatggtgagc
     7141 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     7201 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     7261 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     7321 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     7381 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     7441 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     7501 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     7561 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     7621 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     7681 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     7741 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     7801 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta actgcagccc
     7861 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
     7921 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     7981 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     8041 atgtttcagg ttcaggggga ggtgtgggag gttttttcca actttattat acatagttga
     8101 taattcactg gccgtcgttt tacggtacca tcgatgatga tccagacatg ataagataca
     8161 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8221 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     8281 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     8341 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag atctgcgaag
     8401 atacggccac gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc
     8461 tgatgaatca catacttcct ccattttctt ccactgattg actgttataa tttccctaat
     8521 ttccaggtca aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg
     8581 accaatgaac atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata
     8641 aataacattt aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg
     8701 aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa
     8761 ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat
     8821 ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca
     8881 agtaaaatta ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc
     8941 agcagatcca ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct
     9001 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9061 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9121 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9181 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9841 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10081 acgttaaggc attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10141 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10201 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10261 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10321 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10381 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10441 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10501 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10561 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10621 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10681 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10741 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10801 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10861 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10921 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10981 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11041 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11161 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
    11221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
    11281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
    11341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
    11401 gatggcccac gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat
    11461 gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct ttataggaat
    11521 ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct gttggaccca
    11581 ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag
    11641 acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
    11701 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
    11761 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
    11821 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
    11881 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
    11941 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
    12001 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
    12061 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
    12121 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
    12181 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccctcg
    12241 agcaggaaac agctatgacc atgattacgc caagctatca actttgtata gaaaagttga
    12301 aaattccagt ttgaagaaac ttttcaagac tcaccttttc actgttggt
//
