LOCUS       sequence-325785-       11696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish 
            loss-of-function kcnj13 mutant, T131A and G133R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-T131A-EGFP
FEATURES             Location/Qualifiers
     source          1..11696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5279..5303
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6429..6453)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6452..6461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6458..6460,6461..6463,6464..7174)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6458..6460,6461..6463,6464..7174)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6503..6524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6764..6783)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7111..7132
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7227..7246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7228..7246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7251..7385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7448..7468)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7476..7492)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7508..7642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8081..8268,8269..8280)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8314..8330)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8314..8330)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8367..8387)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8367..8385)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8406..8422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8419..8441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8430..8446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8454..8460,8461..8478,8479..8484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8499..8520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8637..8654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8808..9396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8888..8907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9567..10358,10359..10427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10190..10209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10428..10532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10695..10716)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(11065..11076,11077..11239)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     11612..11628
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11648..11668
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gactcacctt ttcactgttg gtcttaattt aggttgatta tttttgtcaa tttaataatt
       61 acattgtatt gttcatattt tattgtctta ttgtggtgtt tttacagctt ttttattgtt
      121 ctgctttgtt actgtttgcc ttgtatgcat tgtagcgctt tgggtctgag aaaagcgcac
      181 tataaataaa ctgtattatt attattaggg atggacatca cggtggcgcg atgggtggca
      241 caattacctc acagcaagta ggttgctgtt tagagccccg gctggatcag ttggcagttc
      301 tgtgtggagt ttgcatgttc tccctgttca agtaggtttc ctcccggtgc tctggtttcc
      361 cctcacaagt ccaaagacat gatgtatagg tgaatggggt aagctaaatt gaccatagtg
      421 agtgtgtgtg tgtgtgtgaa tgagagtgta taggtgtttc ccagtgttag gttgcaactg
      481 gaaggacatc tgctgtgtaa aacatatgca ggataagttg gcggttcatt cccctatggt
      541 gacccctggt taattaaagg actaagttga aaatgaatga atgaatgaat gattagagat
      601 aaaccgatac cactttttta caaaccgata tgagtacaag tataatagtt tctgttactc
      661 gacaatactg agtaccgatt ctgatacttc ttggatttgg tttcaatgta attaatttga
      721 aagaaggttt tatttcttgc ctgtcgcaag gatgaacaca aaaatcttta ccagaaggat
      781 attttaagct aagaatatac gcacgttgtt gacaaccctg tatcgattac cgattaaacg
      841 tggacattta aaggataagt tatgcttgcg ccatcacaac tttacacatt acatgatcaa
      901 agatcatatt gaatattcgg tttattcgtc agaagcctat agatttgtta aaagtgtatt
      961 ttataaacgg atggtttata aagactaagc tattaatata tcagacattc attaatacat
     1021 caagacatcg atgtcaggta ggacaacaaa tcacatccat gtaatgaaat cttgttgagt
     1081 ggtattggtg cgtggtttca gcatttcatg tgcaatacga gtaagaatat tttaaccaag
     1141 tatcgaccca aaacctgata ctggtatcgg tgcatccctt attattatta ttatcaaaaa
     1201 cacacttgtt ttatttatgt gggtgccaaa caacaaacta tgaaatgcag catgacacgc
     1261 ccaaaaagtg ttcctttgag aacaacttca taaatatgtt tcttatcatt atgcatgaat
     1321 ggtgtactgg gagagctttg gtatcagaat tgagataaga gactttggca ttctaaagga
     1381 caatgacatt atcacactga cagtcaacaa cactttattg ctctggaaat cttatgtcat
     1441 ggggcaaagc tgaggttcat taggacagta ccttatttag agatgaatgg gaaggctgta
     1501 gtgggctgct atcagggaag ggtggtggtc ctcgggggaa ctatggactc acaaaagcac
     1561 atctgctgta acaagatccc ctgtgcaatc tacaaaagtt acaaagagaa gcatggttgg
     1621 ttaaaaaata gaattgactg gatatgctga atgcattgtt tgttgtgtgt tacatcacat
     1681 taaccacaga ttggtgctaa atatttgctt atcttataaa aacctgtgtg tttataaagg
     1741 ggggtgtgga tggatgcttg gcattctttg aaaagatgtt gggggatgga aggaggagac
     1801 cttattgatg agtatccagt ttgcagataa caagttttta gacattcata ctatgatgct
     1861 gtttatgtct gttttttctc attttttcta tacacaaaca gctataaaac attcactttt
     1921 atatctgatg ctgtatatta aatttcgttg tatttattct gtttgctctg ttgggtcata
     1981 gctagggcca gacagaatct gctgacattt tttgcttttt ctgcagagaa ttttgtaaac
     2041 aatctgtgat ttatgcgaga ttttttcggg agtatcgtaa ctaaaaactt actatatgaa
     2101 ataaaaaaaa ataatacctt tttaactttt aataaatata tacaatgcaa gtccacctag
     2161 atccaattat ttggtcgaca aagcaagtct ctcacataat atatcttcta aaagacagaa
     2221 aacattactt tcagaccata ttgtaatcaa atgaacactt tcatattagt caataatatt
     2281 acggaaatgt atttaaaaac tgaataaaca tatatttaca cctttgtctt tatttctaga
     2341 gtgcttttac aatgtagatt gtgtcaaagc agcttaactt agaggattag tatagtaaat
     2401 tgaaactgtg tcagtccatt ttttaagagt ttaagttcag ttcagtttag ttcagtgtga
     2461 gttaattttt actgctgaga gtccaaacac taaagagcaa atccaaatac acaagtcaat
     2521 aaataaactc aatgatgggc taaagatctg gggaaatctg cagatttctg tgcgcacaga
     2581 ttctgtgtgg gcctagtcat tacctatact caacgtttac tctgagaaac ttccatattt
     2641 tagaggtaaa atgctcagaa ttgtaaaaag tcttgtcaaa atacttggaa tttgccaaaa
     2701 acaaaataaa agtcacatca cctcattgtc cttggtatca gcaagcactt ttaacacaat
     2761 cagctgtttt tgtttataac tcccactgaa ggccatgtgc attactccca agttacacag
     2821 taaaaagtca catgtagttt atcatgatca ctgctgctca agcacagctt tatctgttat
     2881 tcagtgtggc aatatctgaa atgtgtgcaa aatattattt attgtagctg atcaccaaca
     2941 ctttcagtgt tttttttttc tacttttaat gagaattatg ttcttaggat ttatccttat
     3001 aaacctcttt aaaaagaaat gttatgtctg catagacacg acatgaaatt atgaacagaa
     3061 aatttaacta tttaagatca agacttgaca tataacattt ctaatgaaca ttaaacctga
     3121 ggtaggccca cttgcaaaaa tgtaactgct atcactcact ttatacatca ttgtgtaact
     3181 tgtacacaca aaaatagtga agcaatgtgg catggaatgc aggtcccatt cattcaaagt
     3241 tattaaagca ctttcttaaa gtgcattact actgagctaa gaccagctag ttgctcatac
     3301 aacatactac aagtagtgtc atcctaattt ggataaaaaa aatgtccatt ttatacatgg
     3361 aaaagtactc attagatccc ccattgtttg tattacggta tttcgtgaac acaagaggta
     3421 aatgacctac agagctgctg ttgtgttaga ttgaaaacac aacacaggat catggaggca
     3481 tccatcatta tgacgcattc tgccacttgt aactctgcac agactttttg aaaagtttaa
     3541 ttgagtcttg gcgttgtcgc agaaaaatac gaaaaattct gtgttaaaat ttgcaaataa
     3601 aaattccagt tttataggaa atcgccatgg ccttgttctt taaccagtta agcttcccct
     3661 tctttcactc tcaagttgca agaagcaagt gtagcaatgt gcacgcgaca gccgggtgtg
     3721 tgacgctgga ccaatcagag cacagagctc cgaaagttta ccttttatgg ctagagccgg
     3781 gtatgtgccg tcatataaaa gagcgcgccc agcttttcag cctcactttg agctcctcca
     3841 cacgcagcta gtgcggaata tcatctgctt gtaacccatt ctcttaagtc gacaaccccc
     3901 caaacccaag gtgagttggt tttacagttt ttaagcgttt aattatagtt tctatctatt
     3961 aatgttaagt aaaatgtgta aatggataaa tatgatttga tgaagtcttt agacagttca
     4021 taacggtctc tttgtagtta tctactaggt ttgatttata agtggtggtt cttttgataa
     4081 tctatgttac atttaataaa tattggctgt atcttaactt ttttgtagcg ttagtggatg
     4141 tggcaggtga gaatgtcggt aatttaacgt gaccagtcgt aggcacgaca ttgaacattg
     4201 aataggccgg tgtgaaatgg gtgttaagtc ttaatataaa gttgtgttct ggctaatctc
     4261 tctttaacag ccataaatgt gatttttaac tacagttttg agggtgctgg tgagcatgtt
     4321 gcacacttga tggatagccg gcatgggaag ttctttgtgc aggcagtgct gcagcagggt
     4381 gtgacctact ttagctagcc ggctaaccag ctctcatctg atgtaacgta aaccccatca
     4441 atctaacatc ggctcattcg tgctttataa gatcgaagat cttgttatta gtaacgggta
     4501 ttggcattta gttttaactt ggttaatttt ctttttgtta tgtgttctat ttaaatgtag
     4561 cctgcagccc cctctgtctt taatgaagct gaagattaac gttaacgtta gtgaagtgtt
     4621 gtatccgcta tgaaagattt gaattttaga ggtgtggtcc actttaataa cggctgcgga
     4681 gttctctggc tgcacttcag ccgctgtgac tgtcgaagca gagcccatca gtttttcagg
     4741 atctcggtga tttttgaggc tagactatga actgaaccga ctgagagtga ccgtccagaa
     4801 atgctttagt gtgaagtctc cgctcacgag gagtccgtgg actgactgca gatctgtgac
     4861 gcagtaacac ccggcggcag acacccgttg aaatgcggtt gtgtaattga tcgcaggcga
     4921 gggtggaaga ggatgtgaaa cttcatttgt gtaaaattta gggagtggtc ctggtgtgat
     4981 gaatgtcgaa atctgttcct ttttactgag ccctacgact ctggctgagt gccacaccgc
     5041 cggcagccgc aaagcggctc aaaccattgc cttttatggt aataatgaga gaatgcagag
     5101 ggacttcctt tgtctggcat atctgaggcg cgcgctgtca ctagcgccca ccagcggtca
     5161 gactgtagaa tgcagagcaa aacaggaagt tgactccaga tggtcacatg cttgctgaaa
     5221 cgcctctccc tggcagcagt gcaattctaa cagtttccct ttcttttaca gttcagcctc
     5281 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatgccta ccaccatgac
     5341 aaacaccacc gctgaccaaa aggcctcctg ccctttgatg gtcaaacctc agcgtcgccg
     5401 cctggtcagc aaagacggtc ggagccagac aagaggaaac acaagaggag gctccagaga
     5461 gacgtgtttc agtgctctgc gggacttgtg gggcacatgg ctggcgctac ggtggcggtg
     5521 ggtggttttg gcattttgtg gctcattcct gcttcactgg ctgctgtttg ctgtattatg
     5581 gtacctgctg gctcgggtca atggagacct ggatgtcctg gatcatgatt ctcctcctcc
     5641 cggtcatgtg ctgtgtgtta aacatgtgaa tgggttcaca gccgcttttt cattcgccct
     5701 ggagacgcaa ctcgctattc ggtatggcac aatgtaccca aatgcagact gtcccactgc
     5761 catcgcgctg cttgccctgc agatgctgct gggactcatg ctggaggcct ttatcactgg
     5821 tgcatttgta gccaagttct ctcggcctca gaagcgatgt gacgggatcc tattcagccc
     5881 tcaggctgtg gtgtgtgagc agaaaggcca gcgctgtcta atgtttcgag tgtgtaacct
     5941 ccagccacag ccgctagtgg acgtatccgt gagtgctgtc ctctacgagg agcgagacga
     6001 tcacgaactc caccaaactg ctctggagtt cagcatcgac aacctgggct ccagatcttg
     6061 ccctcttttc ctctcccctc tgaccttctt tcacccttta aatccatcca ctcctttcat
     6121 caacaaccca agcagccaaa cacactttga gcttgtagtg tttctcactg cgactcagga
     6181 aagcacaggg tctggttacc acaagaggac gtcttacctg cctgacgaga tccaatatgg
     6241 ctactgcttc tccaaggtga cgtctgtaca ccagaacaaa accccaaaca tgcgctattt
     6301 tgacacggtg ccctgtcccc tcacactagc caacacacac accactgata caccggataa
     6361 agagcatgtg gtggttcagc tgaatggaga aggcagcgac cgcgtggaga agaagggtgg
     6421 gcgcgccgac ccagctttct tgtacaaagt ggccaccatg gtgagcaagg gcgaggagct
     6481 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     6541 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6601 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     6661 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6721 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6781 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6841 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6901 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6961 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     7021 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     7081 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     7141 cgggatcact ctcggcatgg acgagctgta caagggcggt ggaagatctg ggaattcaag
     7201 gcctctcgag cctctagatt ctgcagccct atagtgagtc gtattacgta gatccagaca
     7261 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7321 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     7381 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     7441 ttttttccaa ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat
     7501 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     7561 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     7621 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     7681 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     7741 ttatgatcct ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc
     7801 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
     7861 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
     7921 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata
     7981 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg
     8041 caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa
     8101 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa
     8161 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc
     8221 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg
     8281 gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca
     8341 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     8401 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8461 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8581 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8641 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8701 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9241 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca
     9481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
    10561 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    10621 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    10681 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    10741 tcaaagggcg aaaaaccgtc tatcagggcg atggcccacg ctggctaaga actcatcagc
    10801 ctccccggtc catctaccca cgtaccaatg caccaattgg ccacaatgac ggctactaca
    10861 tggtgccatt ccttcctctt tataggaatg gagactacct cctgtccaac aatgctcttg
    10921 gatacgagta cgcctacctg ttggacccag gtcattgcac aacaccagaa atgccctctg
    10981 atctgcaaaa gacgtgaata tctgttcaga cacccatatc cactctgttc cacacaggtc
    11041 agaggtttgt ccaggagttc ttgacagagg tgtaaaaagt actcaaaaat tttactcaag
    11101 tgaaagtaca agtacttagg gaaaatttta ctcaattaaa agtaaaagta tctggctaga
    11161 atcttacttg agtaaaagta aaaaagtact ccattaaaat tgtacttgag tattaaggaa
    11221 gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt taagctttta atctcaaaaa
    11281 acattaaatg aaatgcatac aaggttttat cctgctttag aactgtttgt atttaattat
    11341 caaactataa gacagacaat ctaatgccag tacacgctac tcaaagttgt aaaacctcag
    11401 atttaacttc agtagaagct gattctcaaa attgttagtg tcaagcctag ctcttttggg
    11461 gctgaaaagc aatcctgcag tgctgaaaag cctctcacag gcagccgatg cgggaagagg
    11521 tgtattagtc ttgatagaga ggctgcaaat agcaggaaac gtgagcagag actccctggt
    11581 gtctgaaaca caggccagat gggccctcga gcaggaaaca gctatgacca tgattacgcc
    11641 aagctatcaa ctttgtatag aaaagttgaa aattccagtt tgaagaaact tttcaa
//
