LOCUS       sequence-325797-        1479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from
            a bicistronic mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Perveen S, Khalili Yazdi A, Devkota K, Li F, Ghiabi P, Hajian T, 
            Loppnau P, Bolotokova A, Vedadi M
  TITLE     A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 
            nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19.
  JOURNAL   SLAS Discov. 2021 Jan 10:2472555220985040. doi: 
            10.1177/2472555220985040.
  PUBMED    33423577
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "SLAS 
            Discov. 2021 Jan 10:2472555220985040. doi: 
            10.1177/2472555220985040."
COMMENT     Sequence Label: pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
FEATURES             Location/Qualifiers
     source          1..1479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     974..993
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        974..992
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    993..1017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agctcccagg cttggcaacc aggagttgcc atgcccaact tgtacaaaat gcaacgcatg
       61 cttttggaaa agtgtgacct gcagaattac ggtgacagcg cgactctgcc caaggggatc
      121 atgatgaatg tcgcaaagta tactcagttg tgccagtacc ttaatacgtt gacgctggcc
      181 gtaccgtaca acatgcgcgt aattcacttt ggtgcgggca gcgacaaggg agtcgcgcct
      241 ggcacggctg ttttacgcca atggttaccg actggtacat tgctggttga cagtgacttg
      301 aatgatttcg taagcgatgc cgactcgacg cttatcggcg actgcgccac ggttcataca
      361 gccaacaaat gggacctgat tatttccgat atgtatgacc caaaaaccaa gaatgtgaca
      421 aaggagaacg attcaaagga aggcttcttc acctatatct gtggttttat ccagcagaaa
      481 ttagctcttg gcggttctgt agcaattaag atcacggaac attcctggaa cgcggatctt
      541 tacaagctta tgggccactt cgcgtggtgg accgccttcg tgacgaacgt gaatgcaagt
      601 tcctctgagg cattcttgat cggttgcaat tatcttggaa aacctcgtga gcaaattgat
      661 ggctacgtga tgcacgccaa ctatatcttc tggcgcaaca caaatcccat tcagttatcg
      721 tcctattcct tattcgatat gtctaagttc ccgttgaagt tacgcggcac tgccgtaatg
      781 tcgttgaagg aagggcaaat taatgatatg attttgtcgc ttttatccaa ggggcgttta
      841 atcatccgtg agaataatcg tgtggttatc tcgtctgacg tactggttaa caattgatga
      901 cgaagcttgc ggccgcataa tgcttaagtc gaacagaaag taatcgtatt gtacacggcc
      961 gcataatcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca
     1021 tcttagtata ttagttaagt ataagaagga gatatacata tggccggtaa tgcaaccgaa
     1081 gtgcccgcta acagcactgt tctttccttc tgcgccttcg cggtggatgc ggctaaagcc
     1141 tataaagact atcttgcttc tgggggtcaa cctattacta attgtgtcaa aatgctttgc
     1201 acccacacag ggactggaca ggctattacc gtgacccctg aggctaatat ggatcaggaa
     1261 tcctttggag gcgcgtcctg ttgcctgtac tgccgttgtc atattgacca tcccaatcct
     1321 aaagggtttt gcgacttaaa aggaaagtat gttcaaattc caacgacttg cgcaaatgac
     1381 cccgtgggat tcacattgaa aaacactgta tgcactgtct gcgggatgtg gaaaggatac
     1441 gggtgttcat gcgatcaatt acgcgagcct atgcttcag
//
