LOCUS       sequence-32580-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN27-Pmyo-2::GFP::unc-54_3'UTR
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..58)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaggagacta tacaatgaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggcct
       61 aactaactaa catcggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc
      121 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
      181 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
      241 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
      301 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
      361 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
      421 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
      481 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
      541 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
      601 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
      661 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
      721 ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt
      781 aaatgacaga atacattccc aatataccaa acataactgt ttaaaattaa acatttttc
//
