LOCUS       sequence-325801-        5727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct-expressing iCre AAV Virus. Alias: AiP1010 - 
            pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
FEATURES             Location/Qualifiers
     source          1..5727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      149..604
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(236..255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     446..467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(621..640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     740..762
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(800..818)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        886..990
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(991..1059,1060..1851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1209..1228)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2022..2610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2511..2530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2672..2801
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      3439..3448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3451..3471
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4525..5113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4578..4598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4996..5007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5145..5621
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5344..5363)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   join(5661..5727,1..74)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(5661..5727,1..63)
                     /label=AAV2 ITR
ORIGIN
        1 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
       61 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      121 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
      181 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
      241 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      301 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      361 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      421 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      481 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
      541 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
      601 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      661 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      721 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      781 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      841 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      901 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      961 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1021 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1081 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1141 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1201 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1261 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1321 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1381 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1441 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1501 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1561 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1621 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1681 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1741 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1801 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1861 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1921 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2161 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2401 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2641 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc
     2701 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     2761 gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtataag
     2821 ccttgggggc aatcaaacta ttacattgag tccttggatt tgctacaaat tacattttaa
     2881 atgcaatcat tttataaaag cttcaacact cacacttgga agcgttaccc tgttgaatat
     2941 cactgactca ctaacttgca ttgccatgct aacttgcttt cagagagatc tcagaacaca
     3001 tcatcttctg ctatttcaat acatgcacat taatttccta tcaacgtgtg ctgatcagga
     3061 actctgtaat ctggcaccgg tgtttatttt tattcctgtc tattcctgtt ggctcacgaa
     3121 aagattgttt gagcaagtgt tttatggtga gttgtatcat atgtacattg atttaatctg
     3181 cccacattca gttctacaag cggagccaaa aaaatagaga caagcataat tttcattcaa
     3241 catgagcccc tcaatgcaag ccaagtacct catctggtgc tcagctaaag caacagcaat
     3301 ctgttccacc ctggagacac aactggccac agaaaactta gtgaaaagag gcaatgctat
     3361 gcacaggaca aatgagctcg ggctgggcat aaaagtcagg gcagagccat ctattgctta
     3421 catttgcttc tgggatccgc caccatggtg cccaagaaga agaggaaagt ctccaacctg
     3481 ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga tgaagtcagg
     3541 aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac ctggaagatg
     3601 ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc
     3661 cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag aggcctggct
     3721 gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag atctggcctg
     3781 cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat
     3841 gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga ctttgaccaa
     3901 gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct ggccttcctg
     3961 ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag agtgaaggac
     4021 atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa gaccctggtg
     4081 tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt ggagagatgg
     4141 atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg ggtcagaaag
     4201 aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct ggaagggatc
     4261 tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca gagatacctg
     4321 gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag ggctggtgtg
     4381 tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt gatgaactac
     4441 atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga tggggactaa
     4501 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4561 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4621 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4681 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4741 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4801 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4861 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4921 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc
     4981 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5041 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5101 gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg
     5161 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     5221 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     5281 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     5341 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     5401 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     5461 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     5521 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     5581 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     5641 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     5701 cgctcgctcg ctcactgagg ccgggcg
//
