LOCUS       sequence-325804-        5752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct-expressing iCre AAV Virus. Alias: AiP1046 - 
            pAAV-AiE2013m-minBG-iCre-WPRE-HGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP1046-pAAV-mscRE13-minBGpromoter-iCre-WPRE-h...
FEATURES             Location/Qualifiers
     source          1..5752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      793..802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             805..825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1879..2467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1932..1952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2350..2361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2499..2975
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2698..2717)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3015..3155
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3015..3144
                     /label=AAV2 ITR
     rep_origin      3230..3685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3317..3336)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3527..3548
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3702..3721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3821..3843
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3881..3899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3967..4071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4072..4140,4141..4932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4290..4309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5103..5691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5592..5611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtccca taggcagttt gtggctgagt gctggtccag
      181 gggtgaggag gtgggctatg ttactgaggg tctctgggtg ttaggaaaac agggcccagg
      241 agtctggctg ctcgtatgct ggcccaggct cttgtttttc ttgagctgac ttgctggaga
      301 agtgagctaa gtcagaaaca aaatgccaca ttgcacgccc actgaagtct gggctcaagg
      361 gaaagaagag agattgccag agcgttagct gttcccaatc cactcctgga ccttaagctg
      421 tcttgaacag agttgccaat cagcttggta gggactggcc tttgaggagg ggagggggtg
      481 taggcagggg agggggagag aagggagcag tctgcgctcc atcttaatta cctcatcaga
      541 aacagctccc ttcccgcaaa gctctggtgt cttctacaag agggtgagtc tttggcttta
      601 catgtgaact tgtgccattt gcctgcgtat ataaacatga agggtcgtct gggttcagag
      661 ctgaaatctt tcacttgtga cttagctggg aaattcttgg caagatcaga atgcagtggt
      721 aaggtctgag ctcgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg
      781 cttctgggat ccgccaccat ggtgcccaag aagaagagga aagtctccaa cctgctgact
      841 gtgcaccaaa acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac
      901 ctgatggaca tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg
      961 tctgtgtgca gatcctgggc tgcctggtgc aagctgaaca acaggaaatg gttccctgct
     1021 gaacctgagg atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag
     1081 accatccaac agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc
     1141 ccttctgact ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat
     1201 gctggggaga gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga
     1261 tccctgatgg agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt
     1321 gcctacaaca ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc
     1381 cgcaccgatg gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca
     1441 gctggtgtgg agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct
     1501 gtgtctggtg tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt
     1561 gtggctgccc cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag
     1621 gccacccacc gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg
     1681 tctggccact ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc
     1741 cctgaaatca tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga
     1801 aacctggact ctgagactgg ggccatggtg aggctgctcg aggatgggga ctaagaattc
     1861 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     1921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     1981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2281 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     2341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2401 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2461 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     2521 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     2581 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     2641 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     2701 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     2761 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     2821 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     2881 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     2941 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     3001 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3061 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3121 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     3181 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     3241 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3301 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3361 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3421 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     3481 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3541 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     3601 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3661 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     3721 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3781 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3841 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     3901 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
