LOCUS       sequence-32581-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN27-Pmyo-2::GFP::unc-54_3'UTR
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(42..66)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaagtcttct cctttactca tggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa
       61 acttgggtcg aattcgccct ttttctgtgt ctgacgatcg agggttaaaa tgaaaagtgg
      121 tggagagaag tcaaccgctc ggtgggatgc gcgagcctgg agataacttt gattttcggc
      181 gagcccttct ttttccctgg gtgggtggat tgggaggcgg ggatatccaa ttgtaattct
      241 aggtagctga aaagtgtctg cacactgttc tctaaccatc gacgcaggaa aagtctcttt
      301 ctcaagtgca aatagcaaaa atcttctttc ttccccccca tcttccgagc aaggcgcccc
      361 cacggatagt tgctcgctgg cggtagggag cgaagagagc agagaacctc aacgggatag
      421 tggggatacg agaaaagaaa cggcatttta ggttaactaa agatagtgaa gaagccggct
      481 caatgcagag aggcagacat ccgacggcaa acaacgagag aattttatcc agaacaatga
      541 gtacagtgtg tgcgcgcaaa gtgctcttcc tgccattttg ctactcttat ccacacaacc
      601 actttagcga ttaccaacct atcttgagac ttatctttat tttctcttct tttgccgacg
      661 aaacgggaga gaggggcaaa aaatgaagaa tcgggggcac tgattaacac acttactttt
      721 ttttaggctg aagcacacat tgctgttcgg ggtgtaaagt tcagcagaat tcaaagcaaa
      781 agaccacccg catcggtagt caatattgat ctggaagatt attaaaaatt gtcagagtga
      841 gccatatgca tatgaattt
//
